Celularidade do leite e Unidades Formadoras de Colônias nas mastites causadas por Staphylococcus coagulase positiva e coagulase negativa

Abstract

A mastite é a principal afecção do gado destinado à produção leiteira, que impacta significativamente a cadeia produtiva do leite, com reflexos ainda para a saúde pública. Estudou-se aspectos relacionados à etiologia, celularidade e de contagem bacteriana em 10 propriedades leiteiras, localizadas no Estado Mastitis is the main affection of cattle intended for dairy production, which significantly impacts the milk production chain, with consequences yet to public health. It was studied aspects related to etiology, cellularity and bacterial count in 10 dairy farms, in the State of São Paulo. There were examined 1148 milking cows, totaling 4584 mammary glands. Cases in which there was isolation coagulase positive staphylococci (CPS) and coagulase-negative staphylococci (CNS) were considered. The results showed microbiota with various different pathogens and CNS (128 cases) and CPS (45), Staphylococcus aureus (90), Streptococcus agalactiae (70), Streptococcus dysgalactiae (69), Streptococcus uberis (29), Corynebacterium spp . (230), Klebsiella pneumoniae (28), Klebsiella oxytoca (2), Escherichia coli (15), Enterobacter spp. (3). The somatic cell count (SCC) related to CPS and CNS showed no differences among the evaluated properties, although with significant differences when evaluating the SCC between both groups of staphylococci, as evidenced by comparing discreet CNS and exuberant CPS (P <0.01), discreet CPS and exuberant CPS (P <0.001) and moderate SCN and exuberant SCP (P <0.01). The evaluation of SCC was related to the intensity of infection, considering how slight growth isolation of up to nine colonies, moderate ten to 29 colonies and lush, with 30 or more colonies, revealed for both groups of staphylococci that the higher the number of colony forming units (CFU), SCC is higher being larger in cases of CPS. The results indicate that the importance of both CPS and CNS when considering the high occurrence of the evaluated properties and the fact that it raises the SCC, which compromises the quality of milk. We conclude that when there is a larger number of CFU, is concomitantly higher SCC/ml of milk, in the case of CPS and CNS, which shows a direct relationship of the intensity of the infectious process with the response of milk cellularity, as well as the relevance of these in the etiology of mastitis and negatives aspects to both the production and the quality of milk produced in properties. INDEX TERMS: Milk quality, staphylococci, cellularity, colony forming units.

Evaluation of biofilm formation by bacterial strains isolated from milking equipment and milk samples from cows with mastitis

“…Staphylococcus aureus strains were selected because of their contagious nature and recognition as being the major etiological agent in several cases of bovine mastitis. We also evaluated the presence of E. coli strains commonly found in the environment, the presence of which represents an indicator of environmental contamination (LANGONI et al, 2015). Considering increased recognition of the role of coagulase-negative staphylococci in biofilm production and pathogenicity, S. epidermidis was also chosen as it represents the most important and frequently reported bacteria in the literature (OLIVEIRA et al, 2007).…”

Estratégia de saúde familiar no Brasil: análise microbiológica na sala de vacinação

“…Para tal, foi preciso compreender que as células microbianas, tanto bactérias quanto fungos, crescem formando colónias por meio de agrupamentos (Sales et al, 2014), as quais foram contadas pelo aspecto morfológico, não sendo possível fazer a identificação das espécies microbianas, presentes como contaminantes nos locais de recolha na sala de vacina. Desta forma, foi realizada a contagem aritmética total do número de colónias que cresceram em AN e Asb, e os resultados obtidos foram expressos em unidades formadoras de colónias (UFC) para bactérias (AN) ou para fungos (Asb; Langoni, Guimarães, Costa, Joaquim, & Menozzi, 2015).…”

Comparison phenotypic and genotypic identification of Staphylococcus species isolated from bovine mastitis

“…One of the main agents of bovine contagious mastitis is Staphylococcus aureus (Capurro et al 2010). Nowadays, the intramammary infections caused by other species of the genus Staphylococcus become a serious problem for dairy herds (Ergün et al 2009, Pyorala & Taponen 2009, Unal & Yildirim 2010, Kunz et al 2011, Langoni et al 2015.…”