Caracterização molecular de DNA de Delta papillomavirus bovino (BPV1, 2 e 13) em sarcoides equinos

Abstract

RESUMO.-[Caracterização molecular de DNA de Deltapapillomavirus bovino (BPV1, 2 e 13) em sarcoides equinos.] Sarcoides são tumores fibroblásticos, considerados os tumores de pele mais comuns em pele de equinos e que raramente apresentam regressão espontânea. Papilomavírus bovino (BPV) tipos 1 e 2 são relacionados com a patogenia do sarcoide e, provavelmente, o BPV tipo 13 (BPV13), recentemente descrito, também pode estar associado com a formação dessa lesão. Neste estudo, 20 amostras de lesões cutâneas, sendo 12 constituídas por tecidos frescos e 8 amostras de tecido fixado em formalina e embebido em parafina, provenientes de 15 cavalos foram utilizadas para a identificação do DNA de BPV. A análise histopatológica (HE) confirmou todas as lesões como sarcoide. Para a amplificação do DNA de papilomavírus (PV) foram realizadas três reações de PCR. Como triagem, os primers IFNR2/IDNT2 br Sarcoids are fibroblastic lesions, which are considered as the most common skin tumors of horses; spontaneous regression rarely occurs. The bovine papillomavirus (BPV) types 1 and 2 may be involved in the pathogenesis of sarcoids, and probably the recently described BPV type (BPV13) might be associated with the pathogenesis of this lesion. This study characterized the DNA of BPVs in sarcoids from 15 horses from Brazil by analyzing 20 cutaneous lesions (12 recently collected; 8 from formalin-fixed paraffin-embedded (FFPE) tissues). Histopathology confirmed the proliferative lesions as sarcoids. Three PCRs were performed to amplify papillomavirus (PV) DNA. For screening, the primers IFNR2/ IDNT2 were used to amplify a fragment of the PV L1 ORF. The second primer set was complementary to a common sequence of the E5L2 genomic region of BPV1, 2, and 13. The third primer pair (FAP59/FAP64) targeted a fragment of the PVs L1 ORF. The screening and E5L2 PCRs yielded amplicons in all samples evaluated. The FAP amplicons identified BPV1, 2, and 13 only from fresh tissue samples. The phylogenetic analyses of E5L2 resulted in the identification of BPV1, 2, and 13 in 14 (70%), 2 (10%), and 4 (20%) sarcoids, respectively. Two horses demonstrated multiple lesions: the sarcoids of one of these contained only BPV1 DNA and those of the other contained three types of bovine Deltapapillomavirus (BPV1, 2, and 13). This study confirmed the presence of BPV1, 2, and 13 DNA in equine sarcoids. Moreover, these findings represent the first description of three types of BPV diagnosed in the same horse, as well as the first confirmation of BPV1 and 2 in horses from Brazil.INDEX TERMS: Papillomavirus, Bovine Deltapapillomavirus, BPV1, 2, and 13, DNA, equine sarcoids, horse, skin tumor.

Papilomavirus bovino tipo 2 en un sarcoide equino en Costa Rica

“…El hallazgo de BPV-2 en un sarcoide equino en Costa Rica concuerda con las investigaciones de USA y Brasil, representando además el primer reporte en Costa Rica y Centroamérica (8). La presencia del virus en los sarcoides de los equinos puede explicar los resultados obtenidos por Vindas et al (9), quienes determinaron recidivas en equinos que no recibieron vacuna autóloga en comparación con otros dos animales a los cuales si se les aplicó la autovacuna (9).…”

“…Tanto en Europa como en Australia se ha reportado la presencia del papilomavirus bovino genotipo 1 (BPV-1). En el continente americano en Estados Unidos y Canadá se ha identificado el BPV-2 y en Brasil el BPV-1, BPV-2 y BPV-13 (6,7,8). También en Brasil se reportó la coexistencia de los tres genotipos en un mismo animal de raza equina (8).…”

Detection and Quantification of the E6 Oncogene in Bovine Papillomavirus Types 2 and 13 From Urinary Bladder Lesions of Cattle

“…These primer pairs were able to amplify approximately 120 bp of the E6 ORF (Table 1). Two positive controls (17), one of each BPV type, were amplified using the designed primers. Two conventional PCRs were performed to test these specific E6 primers, and the cycling conditions consisted of an initial denaturing step at 94 • C for 5 min followed by 35 cycles at 94 • C for 1 min, 60 • C for 30 s and 72 • C for 1 min, followed by a final extension at 72 • C for 7 min.…”

“…BPVs are small oncogenic DNA viruses classified as members of the Papillomaviridae family within five different genera: Deltapapillomavirus groups BPVs types 1, 2, 13, and 14; BPVs types 3,4,6,9,10,11,12,15,17,20,23,24,26, and 28 in Xipapillomavirus; BPVs 5, 8, and 25 belong to the Epsilonpapillomavirus genus; BPVs 16, 18, and 22 are classified in Dyokappapapillomavirus; and BPV7, which is the only member of the Dyoxipapillomavirus genus. BPVs 19, 21, and 27 remain unclassified (4).…”

The Detection and Association of Canine Papillomavirus with Benign and Malignant Skin Lesions in Dogs

“…Papillomavirus (PV) can infect and propagate in the cutaneous and mucosal epithelial cells of a wide variety of animal species with a high species specificity [1][2][3]. Although three bovine papillomaviruses (BPV 1, BPV 2, and BPV 13) have been demonstrated to cross-infect the cutaneous fibroblastic cells in equines [4,5], the majority of PVs only infect the epithelium and cause associated lesions [3,6]. To date, more than 50 genera, at least 318 types of PVs, affecting over 54 different animal species have been identified [3,7,8].…”