Treponema denticola in microflora of bovine periodontitis

Borsanelli, Ana Carolina; Júnior, Élerson Gaetti-Jardim; Döbereiner, Jürgen; Dutra, Iveraldo S.

doi:10.1590/s0100-736x2015000300005

Cited by 15 publications

(19 citation statements)

References 20 publications

(13 reference statements)

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“…Prevotella nigrescens is associated with the development of necrotizing gingivitis and periodontitis in humans (Loesche et al 1985, Stingu et al 2013 and was also identified in healthy sites and periodontal pockets of bovines (Borsanelli et al 2015a). In the present study, the frequency of P. nigrescens was significant, being detected in greater number in treated animals.…”

Section: Discussionsupporting

confidence: 52%

“…Thereby, it can be evidenced that bovine oral microbiota is rich and diversified, composed of 395 genera or higher taxa and that microorganisms considered as potential periodontal pathogens are associated with the occurrence of periodontitis in these animals. These include several species of Porphyromonas, Prevotella, and Treponema, as well as Fusobacterium nucleatum, Fusobacterium necrophorum, and Actinomyces naeslundii (Dutra et al 2000, Borsanelli et al 2015a, 2015b, Borsanelli 2017.…”

Section: Introductionmentioning

confidence: 99%

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Efficacy of virginiamycin for the control of periodontal disease in calves

et al. 2019

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Periodontal diseases are multifactorial infectious processes caused by complexes of microorganisms, with damage to health, production, and animal welfare. The aim of the present study was to evaluate the efficacy of virginiamycin in the prevention and control of two early forms of periodontal disease: gingivitis and necrotizing gingivitis. Ten weaned calves, aged four to six months, were permanently kept in a single lot under the same rotational grazing regime in a newly reformed area of Panicum maximum. Five of the calves were orally administered 340mg of virginiamycin (Virginiamycin Group) daily for a period of 18 weeks, while the remaining five calves (Control Group) remained under the same food management but did not receive virginiamycin. During this period, animals underwent 18 weekly evaluations regarding periodontal health, with monitoring and recording of clinical parameters of the eight deciduous incisor teeth on the labial and lingual faces. At approximately two-week intervals, nine collections of subgingival sulcus material from five sites of the four right incisor teeth of each animal were performed and subjected to microbiological evaluation using polymerase chain reaction with primers of 25 microorganisms considered potentially pathogenic. After 1440 periodontal clinical evaluations of incisor teeth of the 10 calves, a total of 395 episodes of gingivitis were recorded, of which 267 occurred in the Control Group and 128 in the Virginiamycin Group. Similarly, 89 episodes of necrotizing gingivitis were recorded; 58 in the Control Group and 31 in the Virginiamycin Group. Comparison of between-group means found significant differences for teeth with gingivitis and necrotizing gingivitis (t test; p<0.05). The total number of teeth with gingivitis (p<0.01) and necrotizing gingivitis (p<0.01) in Control Group was significantly higher than that of gingivitis (p<0.01) and necrotizing gingivitis (p<0.05) in the Virginiamycin Group. There was a positive correlation between total occurrence of gingivitis and necrotizing gingivitis in the Virginiamycin Group by Pearson’s test. Virginiamycin had a protective effect on treated animals compared with the Control Group (OR = 0.36: CI (95%) = 0.27-0.43). In the Control Group, Actinomyces israelli (4.74%), domain Archaea (1.58%), Eikenella corrodens (1.05%), Fusobacterium nucleatum (27.37%), class Mollicutes (5.26%); Porphyromonas endodontalis(5.26%); Porphyromonas gulae(0.53%), Prevotella buccae (6.32%), Prevotella loescheii (3.68%), Prevotella nigrescens (8.42%), Prevotella oralis (1.58%), Tannerella forsythia (0.53%), and Treponema denticola (4.21%) were detected at healthy sites, and gingivitis or necrotizing gingivitis samples. In the Virginiamycin Group, A. israelli (3.41%), domain Archaea (0.98%), F. nucleatum (9.27%), class Mollicutes(4.39%), P. endodontalis (4.39%), P. gulae (0.49%), P. buccae (8.29%), P. loescheii (6.83%), P. nigrescens (15.61%), P. oralis (1.46%), Selenomonas sputigena (0.49%), T. forsythia (0.49%), and T. denticola (2.44%) were detected. In conclusion, virginiamycin administered at a dosage of 340mg/animal/day significantly reduced the occurrence of gingivitis and necrotizing gingivitis in cattle maintained on reformed pastures, and was revealed to have action against periodontal bacterial microbiota considered to be potentially pathogenic.

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Section: Discussionsupporting

confidence: 52%

Section: Introductionmentioning

confidence: 99%

Efficacy of virginiamycin for the control of periodontal disease in calves

et al. 2019

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“…Healthy sites show few or none spirochetes and sites with gingival inflammation but without attachment loss, exhibit low to moderate indexes, whereas deep periodontal pockets harbor a large number of these bacteria (Socransky & Haffajee 2010). T. denticola is considered one of the major periodontopathogens in humans (Mayanagi et al 2004, Foschi et al 2005, Feng & Weinberg 2006, Gaetti-Jardim et al 2010, Benrachadi et al 2012, and it has also been identified in periodontal lesions in bovines, sheep and goats (Borsanelli et al 2015a, 2016, Agostinho 2017, Borsanelli 2017, Campello 2017.…”

Section: Discussionmentioning

confidence: 99%

“…In conventional microbiological analysis of lesions in bovine periodontitis using traditional methods of anaerobic culture, characterization and identification, it is possible to observe that approximately 80% of the microbiota of calves associated with periodontitis was composed of black pigmented colonizing bacteria when cultured in blood agar supplemented with hemin and vitamin K (Botteon et al 1993, Dutra et al 1999. With the use of molecular techniques such as polymerase chain reaction (PCR), which presents high sensitivity and specificity, several species of the genera Porphyromonas, Prevotella and Treponema (Borsanelli et al 2015a(Borsanelli et al , 2015b were identified in bovine periodontitis, considered as potential pathogens in humans, dogs, cats, sheep, and goats.…”

Section: Introductionmentioning

confidence: 99%

Subgingival bacterial microbiota associated with ovine periodontitis

et al. 2019

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Periodontitis is an inflammatory response in a susceptible host caused by complex microbiota, predominantly composed of Gram-negative anaerobic bacteria. Aiming to characterize the subgingival bacterial microbiota associated with ovine periodontitis, polymerase chain reaction (PCR) was performed in subgingival periodontal pocket samples of 14 sheep with severe periodontitis and in subgingival sulcus biofilm of 14 periodontally healthy sheep in search mainly of Gram-negative and Gram-positive microorganisms considered important periodontopathogens. The most prevalent bacteria in the sheep with periodontal lesions were Tannerella forsythia (78.6%), Treponema denticola (78.6%), Fusobacterium nucleatum (64.3%), and Porphyromonas gingivalis (50%), whereas in the healthy sheep, F. nucleatum (42.8%) was the most often detected bacterium. Statistically significant differences were observed for Campylobacter rectus, Enterococcus faecium, Prevotella nigrescens, T. forsythia, and T. denticola (p<0.05) in the sheep with periodontitis in the comparison between groups. Aggregatibacter actinomycetemcomitans, Enterococcus faecalis, and Porphyromonas gulae were not detected in any of the samples analyzed. In conclusion, C. rectus, E. faecium, P. nigrescens, T. forsythia, and T. denticola were associated with severe lesions caused by ovine periodontitis, and F. nucleatum was the most prevalent microorganism in the subgengival sulcus biofilm of healthy sheep.

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“…In general, verification of the presence of Treponema species in oral microbiota linked to periodontitis is restricted when classic culture media and isolation methods are employed. These microorganisms are most often found in periodontal pockets of diseased cattle rather than in healthy animals (Borsanelli and others 2015). Determination of the presence of T amylovorum , T denticola , T maltophilum , T medium and T pectinovorum in periodontitis lesions of sheep represents an original contribution to studies on the aetiopathogenesis of sheep periodontitis.…”

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confidence: 99%