Desenvolvimento do sistema tegumentar em bovinos com idades gestacionais estimadas de 20 a 140 dias

Junior, Horácio Luis Tommasi; Favaron, Phelipe Oliveira; Rodrigues, Rosangela; Guimarães, Juliana Plácido; Miglino, María Angélica

doi:10.1590/s0100-736x2014000700015

Cited by 2 publications

(2 citation statements)

References 28 publications

(21 reference statements)

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“…Weight, diameter of choroid ovoid, and total length in the cranio-caudal direction were measured from the embryos; for the fetuses, weight, length between the base of the nape and the base of the tail, and the length between the withers and the distal far end of thoracic limb were measured to estimate age, using a methodology adapted from Barr et al [ 19 ] and Tommasi Junior et al [ 20 ]. Central nervous system, lung, liver, spleen, bladder, kidney, and reproductive system samples were collected; urine, peritoneal liquid, and abomasal content were obtained through direct puncture using sterile 1 mL syringes.…”

Section: Methodsmentioning

confidence: 99%

The Role of Transplacental Infection in Leptospira spp. Epidemiology in Cattle in Caatinga Biome, Brazil

da Costa Barnabé,

Soares,

Barros

et al. 2024

Microorganisms

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Leptospirosis is an infectious disease that affects domestic animals, wild animals, and humans. It represents a public health problem and has an important economic impact on livestock. This study aims to investigate the importance of genital and transplacental infection in the epidemiology of leptospirosis in cows maintained in Caatinga biome conditions, Northeastern Brazil, as well as reporting organs colonized by Leptospira spp. in embryos and fetuses. Blood, urinary tract (urine, bladder, and kidney), and reproductive tract (vaginal fluid, uterus, uterine tube, ovary, and placenta) samples were collected from 15 slaughtered pregnant cows. Two embryos and 13 fetuses were sampled. Central nervous system and choroid ovoid samples were collected from embryos. Blood, central nervous system, lung, peritoneal liquid, abomasal content, liver, spleen, urine, bladder, kidney, and reproductive system samples were collected from fetuses. Diagnostic methods included the microscopic agglutination test (MAT) using a collection of 24 serovars belonging to 17 different pathogenic serogroups of five species as antigens, as well as polymerase chain reaction (PCR). Anti-Leptospira spp. antibodies were found in 9 cows (60%), while 13 cows (86.67%) had at least one organ or urine with leptospiral DNA. No fetus was seroreactive. Among the embryos and fetuses, 13 (86.67%) presented leptospiral DNA, proving a high frequency of transplacental infection (100%). For cows, the most frequent biological materials regarding Leptospira spp. DNA detection were placenta (13 out of 15 samples; 86.7%), uterus (10 out of 15 samples; 66.7%), and vaginal fluid (5 out of 15 samples; 33.3%), while, for fetuses/embryos, the most frequent PCR-positive samples were choroid ovoid (1/2; 50%), spleen (6/13; 46.2%), kidney (5/13; 38.5%), and central nervous system (5/15; 33.3%). Sequenced samples based on the LipL32 gene presented 99% similarity with L. borgpetersenii. The results indicate that transplacental infection is an efficient way of spreading Leptospira spp. in cows maintained in Caatinga biome conditions. Therefore, prevention and control strategies must include actions that interrupt transmission through this alternative route.

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Section: Methodsmentioning

confidence: 99%

The Role of Transplacental Infection in Leptospira spp. Epidemiology in Cattle in Caatinga Biome, Brazil

da Costa Barnabé,

Soares,

Barros

et al. 2024

Microorganisms

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“…Навіть фібробласти з різних шарів дерми відрізняються за своїми біосинтетичними, функціональними та культуральними властивостями, експресією генів (Sorrell & Caplan, 2004;Janson et al, 2012;Rippa et al, 2019). При чому, ці відмінності закладаються ще у пренатальному онтогенезі та, зокрема, проявляються в їхньому асинхронному рості (Smith et al, 1982;Tommasi Junior et al, 2014).…”

Section: рис 2 динаміка приросту товщини папілярного шару дерми шкіри...unclassified

Comparative histomorphometry of postnatal skin development of heifers of 4 breeds of base and initial genotypes

Kyrylovskyy

2022

SMLNUVMB

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The total thickness of the skin of the heifers of the four groups increased from 3 to 18 months of postnatal ontogenesis, depending on the breed, by 16–27 %. In the heifers of the base and original genotype breeds, different periods of completion of the growth of the skin thickness were established. In all four groups of heifers, there were fundamental differences in the dynamics of the growth of the thickness of the outer integument. The thickness of the epidermal layer of the skin reached maximum values in the early postnatal ontogeny of heifers. The thickness of the layers of the dermis of the heifers of the four groups increased with age, depending on the breed, by 33–43 % – papillary and 9–24 % – reticular. In the postnatal ontogenesis of the heifers of the four groups, the noticeable increase (more than 50 %) of the papillary layer of the dermis occurred in the early age period of 3–6 months, and the dynamics of changes in the thickness of the reticular layer depended on the breed of heifers. Structural transformations of its internal organization accompanied the postnatal development of the skin of the heifers of the four groups – the ratio of skin layers changed within 5–8 %, depending on the breed. In the dynamics of postnatal ontogenesis of heifers of the base and original genotype breeds, reliable differences in the thickness of the epidermis and the reticular (together with the subcutaneous base) skin layer was manifested, as well as the parameters of the growth ratio of the papillary/reticular layers of the dermis. The dependence of the parameters of the thickness of the epidermal barrier of the skin of heifers of the four groups on the period of the year and the seasonal differences in the growth of the papillary and reticular (together with the subcutaneous base) layers of the skin of heifers of the base and original genotype breeds indicate the probable corrective effect of environmental factors on the morphology of the skin of heifers and the difference in the manifestations of adaptation.

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Desenvolvimento do sistema tegumentar em bovinos com idades gestacionais estimadas de 20 a 140 dias

Cited by 2 publications

References 28 publications

The Role of Transplacental Infection in Leptospira spp. Epidemiology in Cattle in Caatinga Biome, Brazil

The Role of Transplacental Infection in Leptospira spp. Epidemiology in Cattle in Caatinga Biome, Brazil

Comparative histomorphometry of postnatal skin development of heifers of 4 breeds of base and initial genotypes

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