NaOCl effect on biofilm produced by Staphylococcus aureus isolated from the milking environment and mastitis infected cows

Abstract

Biofilms constitute a physical barrier, protecting the encased bacteria from detergents and sanitizers. The objective of this work was to analyze the effectiveness of sodium hypochlorite (NaOCl) against strains of Staphylococcus aureus isolated from raw milk of cows with subclinical mastitis and Staphylococcus aureus isolated from the milking environment (blowers and milk conducting tubes). The results revealed that, in the presence of NaOCl (150ppm), the number of adhered cells of the twelve S. aureus strains was significantly reduced. When the same strains were evaluated in biofilm condition, different results were obtained. It was found that, after a contact period of five minutes with NaOCl (150ppm), four strains (two strains from milk , one from the blowers and one from a conductive rubber) were still able to grow. Although with the increasing contact time between the bacteria and the NaOCl (150ppm), no growth was detected for any of the strains. Concerning the efficiency of NaOCl on total biofilm biomass formation by each S. aureus strain, a decrease was observed when these strains were in contact with 150 ppm NaOCl for a total period of 10 minutes. This study highlights the importance of a correct sanitation protocol of all the milk processing units which can indeed significantly reduce the presence of microorganisms, leading to a decrease of cow´s mastitis and milk contamination.

Climatic and regional antibiotic resistance patterns of Staphylococcus aureus in South African dairy herds

“…Challenging weather conditions and climatic differences may cause bacteria to form protective mechanisms such as biofilm which might cause an increase in the prevalence of antibiotic resistance (Da Silva Meira et al 2012; Melo et al 2014). Many normal microbiota bacteria produce a capsular polysaccharide matrix (glycocalyx) to form a biofilm.…”

Anti-biofilm activity of hydromethanolic plant extracts against Staphylococcus aureus isolates from bovine mastitis

“…More specifically, the production of slime and biofilms represents a serious problem, namely in the dairy chain, since bacteria incorporated in its own matrix, can survive the usual sanitation and cleaning process [2]. In this sense, and taking into account that the presence of biofilms in the dairy industry must be avoided due to the risk of milk contamination, food poisoning and even occurrence/recurrence of BM [17], the discovery of effective antimicrobial agents for control dairy industry pathogens is of paramount importance, namely those targeting microbial biofilms.…”

Avaliação Da Qualidade Do Leite Cru Durante Acompanhamento Técnico Nas Propriedades Rurais Do Município De Perobal-Pr

“…As boas práticas para a obtenção de uma ordenha higiênica devem ser estabelecidas, com o intuito de prevenir novos casos de mastite nos rebanhos. Melo et al (2014) isolaram linhagens de S. aureus do ambiente de ordenha (borrachas de ordenhadeiras e mangueiras condutoras de leite). Ao testarem o efeito do hipoclorito de sódio contra essas linhagens, constataram que o contato superficial dos materiais com o hipoclorito de sódio a 150 ppm por dez minutos impediu o desenvolvimento da população bacteriana e produção de biofilme.…”

“…Todavia, a mastite tem um importante papel na qualidade microbiológica do leite, pois o processo infeccioso e inflamatório pode modificar a composição do leite, aumentando a contagem de células somáticas e até mesmo causar a morte de vaca, levando a grandes perdas econômicas (MELO et al, 2014).…”