Produção e purificação de imunoglobulinas Y policlonais anti-Leptospira spp.

Tavares, Tatiane Cristina Fernandes; Soares, Pollyanna Mafra; Neves, João H. F. F.; Soares, Michel S.; Júnior, Álvaro Ferreira; Souza, Dayane Lorena Naves de; Ávila, Veridiana Melo Rodrigues; Lima-Ribeiro, Anna Monteiro Correia

doi:10.1590/s0100-736x2013000900008

Cited by 3 publications

(2 citation statements)

References 27 publications

(21 reference statements)

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“…This study demonstrated that hens immunized with strain B19 produced IgY antibodies reactive to this antigen, which were detectable by indirect ELISA and by official tests for the diagnosis of bovine brucellosis, BAPA and 2-ME, representing a good choice for the production the polyclonal antibodies, like showed in study with the production of high titers of specific polyclonal IgY antibodies to Leptospira [24].…”

Section: Discussionmentioning

confidence: 54%

Production and Evaluation of Immunoglobulin Y Anti-Brucella abortus (Vaccinal Strain B19)

Soares

Lourencetti

et al. 2018

Acta Scientiae. Vet.

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Background: The immunoglobulin Y is a principal antibody current in the blood of hens, which are transferred from the maternal blood serum to the egg yolk. The extraction of IgY from the egg yolk apply animal welfare when compared to the extraction of IgG, reducing the number of animals and prevent a bleeding of hens through the extraction of the IgY from eggs, besides that IgY presenting high specificity for antigenic binding. The objective of this study was to produce specific polyclonal IgY antibodies anti-Brucella abortus by immunizing hens with B19 vaccine and evaluate their reactivity through Buffered Acidified Plate Antigen (BAPA), 2-Mercaptoethanol (2-ME) and indirect ELISA diagnostic tests.Materials, Methods & Results: Four 25-week-old White Leghorn hens were immunized, two of them comprising the control group (Group 1) with phosphate-buffered saline (PBS) with adjuvant, and the others two immunized with B19 vaccine (Brucella abortus vaccine strain B19), representing the Group 2. The immunizations occurred six times with a 15-day interval between each. Blood samples were taken biweekly (seven times); and daily, the eggs were collected for 13 weeks, the first collection of blood and eggs, performed one week before the first immunization of each group. The IgY was purified from egg yolk, using the method of dilution in acid water and precipitation with ammonium sulfate for delipidation. BAPA, 2-ME and ELISA tests performed to verify the specificity of IgY confirmed the reactivity of polyclonal antibodies specific to the antigen used both in blood serum samples and in the purified egg yolks. The hens from the control group did not present reactivity in the diagnostic tests used, which was already expected, since no antigen was used in any of their immunizations. Hens immunized with the Brucella abortus B19 vaccine produced detectable reactive antibodies in the three tests used on blood serum and IgY samples extracted from the egg yolk. In Group 2 (vaccine B19), blood serum samples started to react one week after the first inoculation, and the IgY samples extracted from the egg yolk were reagent two weeks after serum IgY appear reactivity, showing the transfer of specific antibodies to the egg yolk, was late.Discussion: Although the transfer of serum Igy to egg yolk was late when compared to others authors which found that the transovarian passage of immunoglobulin Y occurred in approximately three to six days after IgY being detected in blood serum, the results of this study showed the occurrence of the transfer of blood serum IgY anti-Brucella abortus to egg yolk of hens immunized with B19 vaccine, the same found by others researches found the same results with others antigens. Thus, it can be concluded that immunoglobulins Y produced in this study can be used as specific antibodies in diagnostic tests for the detection of the Brucella abortus antigen, in addition, this process guarantees the welfare of the animal, since it avoids bleeding and it is possible to obtain high concentrations of antibodies directly from the hen egg, which is a great advantage, because IgY can be easily isolated from the egg yolk by the precipitation technique discarding the need of invasive and painful procedures that involve bloody interventions to obtain the serum antibodies like occur in mammals for extraction of IgG.

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Section: Discussionmentioning

confidence: 54%

Production and Evaluation of Immunoglobulin Y Anti-Brucella abortus (Vaccinal Strain B19)

Soares

Lourencetti

et al. 2018

Acta Scientiae. Vet.

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“…Anticorpos específicos, policlonais ou monoclonais, para diagnóstico, pesquisa e terapias tem sido obtido especialmente de mamíferos tais como coelhos e ratos (Tavares et al, 2013). Batista et al (2019) os anticorpos são ferramentas biotecnológicas de alto valor, representando o segmento de maior crescimento no mercado de produtos biológicos alcançando atualmente.…”

Section: Resultsunclassified

Pseudomonas aeruginosa: caracterização antigênica e produção anticorpos

Ribeiro¹

2020

J. Biotechnol. Biodivers.

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A Pseudomonas aeruginosa destaca-se ao longo dos anos entre os agentes infecciosos frequentemente isolado em ambientes hospitalares. Atualmente as infecções hospitalares causadas por P. aeruginosa representam um desafio para a terapêutica antimicrobiana. Uma alternativa frente aos micro-organismo multirresistentes tem sido a busca de vacinas. O presente trabalho visa verificar através de revisão de literatura o que há de concreto e as perspectivas concernentes a caracterização de antígenos de P. aeruginosa e, por conseguinte a produção e aplicabilidade de anticorpo. Fez-se um levantamento bibliográfico em periódicos das línguas portuguesa e inglesa entre os anos 1980 a 2019 sendo analisados e inseridos no trabalho 24 obras. No âmbito vacinal, os recursos disponíveis se baseiam em antígenos oriundo de toxinas de fatores de virulência conhecidos de espécies de P. aeruginosa, tais como; exotoxina-A, proteases e elastases, e componentes do LPS. Notou-se uma escassez na literatura quanto a temática. Pesquisas devem ser conduzidas na caracterização de antígenos e por conseguinte produção de anticorpos.

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