Molecular epidemiology and extended-spectrum β-lactamases production of Klebsiella pneumoniae isolated from three dairy herds

Abstract

Pesq. Vet. Bras. 33 (7) The objectives of this study were to isolate Klebsiella pneumoniae from different sources in three dairy cattle herds, to use the pulsed-field gel electrophoresis (PFGE) to measure genotypic similarities between isolates within a dairy herd, to verify the production of extended-spectrum β-lactamases (ESBLs) by the double-disk synergy test (DDST), and to use the PCR to detect the main ESBLs subgroups genes. Three dairy farms were selected based on previous mastitis outbreaks caused by K. pneumoniae. Milk samples were collected from lactating cows and from the bulk tank. Swabs were performed in different locations, including milking parlors, waiting room, soil, animal's hind limbs and rectum. K. pneumoniae was isolated from 27 cases of intramammary infections (IMI) and from 41 swabs. For farm A isolates from IMI and bulk tank were considered of the same PGFE subtype. One isolate from a bulk tank, three from IMI cases and four from environmental samples were positive in the DDST test. All eight DDST positive isolates harbored the bla shv gene, one harbored the bla tem gene, and three harbored the bla ctx-m gene, including the bulk tank isolate. Our study confirms that ESBL producing bacteria is present in different locations in dairy farms, and may be responsible for IMI. The detection of ESBLs on dairy herds could be a major concern for both public and animal health.INDEX TERMS: Dairy cattle herds, extended-spectrum β-lactamases (ESBLs), Klebsiella pneumoniae, pulsed-field gel electrophoresis (PFGE). lidades em três propriedades leiteiras, utilizar a eletroforese em campo pulsátil para averiguar similaridades genotípicas entre os isolados de uma mesma propriedade, verificar a produção de beta-lactamases de espectro estendido (ESBLs) pela prova da disco-difusão dupla associada (DDST) e utilizar a PCR para detecção dos principais subgrupos genéticos de ESBLs. Três propriedades leiteiras foram selecionadas baseando-se em surtos prévios de mastites causadas por K. pneumoniae.

Presencia de Enterobacteriaceae productoras de betalactamasa de espectro extendido (ESBL) en leche de tanque a granel de granjas lecheras bovinas en Antioquia, Colombia

“…The frequency of ESBL-producing Enterobacteriaceae reported in this study (3.3%) agrees with other studies conducted in bulk-tank milk samples in Switzerland, Brazil, India, the Czech Republic, Indonesia, and Germany, which reported prevalences ranging between 0 and 9% (Geser et al, 2012;Nóbrega et al, 2013;Rasheed et al, 2014;Skočková et al, 2015;Sudarwanto et al, 2015;Odenthal et al, 2016).…”

MOLECULAR CHARACTERIZATION AND IMMUNOPROTECTIVE ACTIVITY OF CAPSULAR POLYSACCHARIDE OF <I>KLEBSIELLA PNEUMONIAE</I> ISOLATED FROM FARM ANIMALS AT TAIF GOVERNORATE

“…Moreover there was great difference Science Publications AJID between the prevalence of Klebsiella isolated from various apparently healthy animal origins, as it was higher in sheep, followed by cow, goats and lastly camels with percentages of 8.5, 5.5, 4.1 and 3.9%, respectively (Table 3). These findings support the statement by Diego et al (2013) who reported that, the natural flora of the intestine includes a large population of coliform bacteria including Klebsiella pneumoniae. Faeces are an important source of these organisms in the environment.…”

“…The most common response of the cell to antibiotics is to cease growing (bacteriostasis), but classes of compounds such as β-lactams, continued growth is permitted, with inhibition of the target in the organism leading indirectly to cell death. There has been growing concern about antimicrobial resistance for some years now (Diego et al, 2013). The antibiogram of pathogens could be variable from place to place and from case to another.…”

Prevalence and Antibiogram of Generic Extended-Spectrum β-Lactam-Resistant Enterobacteria in Healthy Pigs

“…The 67.4% Klebsiella isolation prevalence suggested that the genus may have the capacity of acquiring genes encoding for ESBLs more than Salmonella and E. coli with isolation prevalence of 23.9% and 8.7%, respectively. Among ESBL-resistant enterobacteria, Klebsiella acquired the greatest variety of genes encoding for ESBLs production and it produced the greatest variety of ESBLs (Paterson et al, 2003;Nobrega et al, 2013). Studies conducted elsewhere in Africa also reported isolation of ESBL-resistant Klebsiella more frequently than any other genus in Enterobacteriaceae (Blomberg et al, 2005;Gangoue-Pieboji et al, 2005).…”