Sequencing and expression analysis of hepcidin mRNA in donkey (Equus asinus) liver

Filho, José Paes de Oliveira; Marques, J. A.; Cunha, Paulo Henrique Jorge da; Medeiros, Gildenor Xavier; Riet-Correa, Franklin; Machado, Vânia Maria Vasconcelos; Borges, Alexandre Secorun

doi:10.1590/s0100-736x2012001000019

Cited by 2 publications

(2 citation statements)

References 22 publications

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“…Hep 25 contains eight cysteine residues that form four disulfide bridges important for maintaining correct peptide folding and stabilizing the β-hairpin structure. Hep 25 is highly conserved in vertebrate species, particularly the eight cysteine residues and the N-terminal region containing the ATCUN motif [16,17]. Importantly, the ATCUN motif (H2N-X-X-H, where X is variable) is capable of specifically binding Cu(II) and Ni(II) metals.…”

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confidence: 99%

Human Antimicrobial Peptide Hepcidin 25-Induced Apoptosis in Candida albicans

Chen

Lan

2020

Microorganisms

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Hepcidin 25 (hep 25) is a cysteine-rich 25-amino acid antimicrobial peptide containing the amino-terminal Cu(II)/Ni(II)-binding (ATCUN) motif. Upon metal binding, the ATCUN motif is known to be involved in the generation of reactive oxygen species (ROS), especially hydrogen peroxide and hydroxyl radicals, which act against different bacterial species. However, the antifungal activity and its correlation to the Cu(II)-ATCUN complex of Hep 25 are still poorly understood. Here, we found that ROS accumulation plays an important role in the fungicidal activity of hep 25 against Candida albicans. In addition, Annexin V-FITC staining and TUNEL assay results provide clues about the apoptosis induced by hep 25. Moreover, hep 25 also increases the generation of ROS, possibly because of copper binding to the ATCUN motif, which is relevant to its activity against C. albicans. Finally, the C. albicans killing action of hep 25 is an energy- and temperature-dependent process that does not involve targeting the membrane. Taken together, our results provide new insights into the mechanisms of hep 25 against C. albicans cells and the potential use of hep 25 and its derivatives as novel antifungal agents.

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confidence: 99%

Human Antimicrobial Peptide Hepcidin 25-Induced Apoptosis in Candida albicans

Chen

Lan

2020

Microorganisms

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“…A research on donkey identified that the high expression of hepcidin in the liver was found to be similar to the reference gene expression. The study also stated that the mature hepcidin sequence from the donkey exhibited 100% sequence homology to the mature hepcidin sequence from the horse [ 111 ].…”

Section: Equine Ampsmentioning

confidence: 99%

Antimicrobial Peptides in Farm Animals: An Updated Review on Its Diversity, Function, Modes of Action and Therapeutic Prospects

Kumar

Ali

Singh

et al. 2020

Veterinary Sciences

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Antimicrobial peptides (AMPs) are the arsenals of the innate host defense system, exhibiting evolutionarily conserved characteristics that are present in practically all forms of life. Recent years have witnessed the emergence of antibiotic-resistant bacteria compounded with a slow discovery rate for new antibiotics that have necessitated scientific efforts to search for alternatives to antibiotics. Research on the identification of AMPs has generated very encouraging evidence that they curb infectious pathologies and are also useful as novel biologics to function as immunotherapeutic agents. Being innate, they exhibit the least cytotoxicity to the host and exerts a wide spectrum of biological activity including low resistance among microbes and increased wound healing actions. Notably, in veterinary science, the constant practice of massive doses of antibiotics with inappropriate withdrawal programs led to a high risk of livestock-associated antimicrobial resistance. Therefore, the world faces tremendous pressure for designing and devising strategies to mitigate the use of antibiotics in animals and keep it safe for posterity. In this review, we illustrate the diversity of farm animal-specific AMPs, and their biochemical foundations, mode of action, and prospective application in clinics. Subsequently, we present the data for their systematic classification under the major and minor groups, antipathogenic action, and allied bioactivities in the host. Finally, we address the limitations of their clinical implementation and envision areas for further advancement.

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Sequencing and expression analysis of hepcidin mRNA in donkey (Equus asinus) liver

Cited by 2 publications

References 22 publications

Human Antimicrobial Peptide Hepcidin 25-Induced Apoptosis in Candida albicans

Human Antimicrobial Peptide Hepcidin 25-Induced Apoptosis in Candida albicans

Antimicrobial Peptides in Farm Animals: An Updated Review on Its Diversity, Function, Modes of Action and Therapeutic Prospects

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