Lipocalina associada à gelatinase de neutrófilos (NGAL) e calprotectina no tecido laminar de equinos após obstrução jejunal, tratados ou não com hidrocortisona

Laskoski, Luciane Maria; Valadão, C. A. A.; Vasconcelos, Rosemeri de Oliveira; Faleiros, Rafael Resende; Mendes, Heloisa Maria Falcão; Ferrucci, Danilo Lopes; Silva, Juliete A.F.; Machado, Dagmar D.R.S.

doi:10.1590/s0100-736x2012000900001

Cited by 3 publications

(2 citation statements)

References 58 publications

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“…The NGAL protein is comprised of 178 amino acids, and it occurs in a monomeric and a homodimeric form with molecular weights of 25 kDa and 46 kDa, respectively . Using mass spectometry, NGAL was identified in hoof lamellar tissues of laminitic horses . Bundgaard et al identified NGAL in necrotic cecums, inflamed salivary glands, and wounds of horses.…”

Section: Introductionmentioning

confidence: 99%

Validation of an ELISA for detection of neutrophil gelatinase‐associated lipocalin (NGAL) in equine serum

Jacobsen

Berg

Tvermose

et al. 2018

Veterinary Clinical Pathol

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Background: Neutrophil gelatinase-associated lipocalin (NGAL) has been shown to be a useful marker of kidney injury in people and dogs, but has not been described in horses. Objectives:The aim of the study was to validate a commercially available porcinespecific ELISA to measure serum concentrations of equine NGAL.Methods: Intra-and interassay imprecisions were evaluated by multiple measurements on equine serum pools. Assay inaccuracy was determined by the linearity under dilution. Overlapping performance was assessed by measuring NGAL concentrations in horses with normal and elevated serum creatinine levels.Results: Intra-and interassay imprecision (coefficient of variation) ranged from 5.35% to 28.39%. The ELISA showed no signs of inaccuracy. Overlapping performance was acceptable, as the assay was able to detect the expected differences of NGAL levels in horses with normal and elevated serum creatinine concentrations. Conclusions:Equine serum NGAL concentrations could be quantified reliably using the porcine-specific ELISA.| 603 monitoring tool in patients with kidney injury. 10 Diagnostic use of NGAL has not been described in horses.The aim of this study was to validate a commercially available, porcine-specific ELISA for measurements of NGAL in equine serum by investigating (a) assay characteristics (imprecision, inaccuracy, and detection limit [DL]), and (b) the ability of the assay to detect the expected differences in NGAL levels between the two selected equine populations, one with elevated and one with normal serum creatinine concentrations. | MATERIALS AND METHODS | The NGAL assayThe assay, a commercially available ELISA (porcine NGAL ELISA kit, cat. no. KIT 044; Bioporto, Hellerup, Denmark) developed for measuring NGAL concentrations in porcine urine, plasma, serum, tissue extracts, and culture media was used for heterologous NGAL determination in equine serum samples.The analyses were performed using a Multiskan EX plate reader (Thermo Electron Corporation, Hvidovre, Denmark) supported with the Ascent software, Version 2.6 for the iEMS Reader MF according to the manufacturer's instructions. Absorbance was read at 450/ 620 nm. All samples were run in duplicate. The calibration curve was made using the calibrator supplied with the kit and fitted with a four-parameter logistic curve using the freely available software at www.myassays.com. When optical density values were outside the calibration curve, samples were further diluted and reanalyzed.

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Section: Introductionmentioning

confidence: 99%

Validation of an ELISA for detection of neutrophil gelatinase‐associated lipocalin (NGAL) in equine serum

Jacobsen

Berg

Tvermose

et al. 2018

Veterinary Clinical Pathol

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“…Increased NGAL concentrations are seen in human patients with suspicion of AKI (14,24). In horses, NGAL was identified in postmortem specimens of laminitic hoof lamellar tissue, necrotic cecum, inflamed salivary glands and wounds (3,10). So far, there are only two reports on serum NGAL concentration in healthy horses and horses with renal problems (9,27).…”

Section: Discussionmentioning

confidence: 99%

Evaluation of serum and urine neutrophil gelatinase-associated lipocalin and cystatin C as biomarkers of acute kidney injury in horses

Siwińska

Żak

Pasławska

2021

Journal of Veterinary Research

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Introduction Diagnosis of acute kidney injury (AKI) in horses is difficult at the subclinical stage, due to nonspecific clinical signs. The aim of this study was to evaluate the concentrations of selected serum and urinary biomarkers in healthy horses, horses at risk of AKI, and those with clinical AKI. Material and Methods Thirty healthy horses, 30 horses at risk of AKI and 11 horses with clinical AKI and azotaemia were included in the study. Serum and urinary neutrophil gelatinase-associated lipocalin (NGAL) and cystatin C were measured using commercially available enzyme immunoassay tests. Results The median and (in parentheses) first and third quartile concentrations of selected biomarkers in healthy horses, horses at risk of AKI and horses with AKI were respectively as follows: serum cystatin C – 0.25 (0.19–0.37), 0.23 (0.15–0.37) and 0.61 (0.37–1.13) mg/L; serum NGAL – 50.5 (38.8–58.8), 51.1 (40.4–66.9) and 98.1 (59.4–128.2) ng/mL; urinary NGAL – 20.7 (17.9–24.5), 32.3 (32.7–55.8) and 36.6 (26.8–89.9) ng/mL; and urinary cystatin C – 0.1 (0.07–0.13), 0.13 (0.1–0.2) and 0.34 (0.22–0.37) mg/L. There were significant differences in the concentration of all biomarkers between the healthy and AKI-affected horses. Conclusion Horses with AKI all had biomarker concentrations higher than the healthy horses. None of the biomarkers made azotaemia recognisable in all affected horses. The obtained results indicate the need to create a serum and urinary biomarker panel to detect AKI.

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Systemic leukopenia, evaluation of laminar leukocyte infiltration and laminar lesions in horses with naturally occurring colic syndrome

Laskoski

Locatelli-Dittrich

Valadão

et al. 2015

Research in Veterinary Science

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Lipocalina associada à gelatinase de neutrófilos (NGAL) e calprotectina no tecido laminar de equinos após obstrução jejunal, tratados ou não com hidrocortisona

Cited by 3 publications

References 58 publications

Validation of an ELISA for detection of neutrophil gelatinase‐associated lipocalin (NGAL) in equine serum

Validation of an ELISA for detection of neutrophil gelatinase‐associated lipocalin (NGAL) in equine serum

Evaluation of serum and urine neutrophil gelatinase-associated lipocalin and cystatin C as biomarkers of acute kidney injury in horses

Systemic leukopenia, evaluation of laminar leukocyte infiltration and laminar lesions in horses with naturally occurring colic syndrome

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