Progesterone and Follicle Stimulating Hormone interact and promote goat preantral follicles survival and development in vitro

Abstract

We investigated the effects of progesterone and follicle stimulating hormone (FSH) on survival and growth of caprine preantral follicles. Pieces of ovarian tissue were cultured for 1 or 7 days in minimum essential medium (MEM) alone or containing progesterone (1, 2.5, 5, 10 or 20ng/mL), FSH (50ng/mL) or the interaction between progesterone and FSH. Fresh (non-cultured control) and cultured ovarian tissues were processed for histological and ultrastructural studies. After 7 days the addition of FSH to all progesterone concentrations maintained the percentage of normal follicles similar to fresh control. At day 7 of culture, a higher percentage of developing follicles was observed only in 2.5ng/ml of progesterone associated with FSH or 10ng/ml of progesterone alone when compared with control. From day 1 to day 7 of culture, a signiϐicant increase in the percentage of developing follicles was observed in MEM and 2.5ng/ml of progesterone + FSH. In addition, after 7 days, in all treatments, there was a signiϐicant increase in follicular diameter when compared with control, except for MEM alone and in 5ng/ml of progesterone + FSH or 10ng/ml of progesterone alone. Ultrastructural studies conϐirmed follicular integrity after 7 days of culture in 2.5ng/ml of progesterone with FSH. In conclusion, this study demonstrated that the interaction between progesterone and FSH maintains ultrastructural integrity, stimulates primordial follicles activation and further growth of cultured caprine preantral follicles. tos de tecido ovariano foram cultivados por 1 ou 7 dias em Meio Essencial Mínimo (MEM) sozinho ou contendo progesterona (1, 2.5, 5, 10 ou 20ng/mL), FSH (50ng/mL) ou a combinação entre esses dois hormônios. O tecido fresco (controle não-cultivado) e o cultivado foram processados para análise histológica e ultra-estrutural. Após 7 dias a adição de FSH a todas as concentrações de progesterone manteve o percentual de folículos normais similar ao controle fresco. No dia 7 de cultivo, um alto percentual de folículos em desenvolvimento foi observado somente no tratamento com 2,5ng/ml de progesterona associada ao FSH ou com 10ng/ml de progesterona sozinha, em relação ao controle fresco. Do dia 1 para o dia 7 de cultivo, um aumento signiϐicativo no percentual de folículos em desenvolvimento foi observado no MEM sozinho e adicionado de 2,5ng/ml de progesterona + FSH. Além disso, após 7 dias, Pesq. Vet. Bras. 32(4):361-367, abril 2012 362Isabel B. Lima-Verde et al.em todos os tratamentos, houve um aumento signiϐicativo no diâmetro folicular em relação ao controle, exceto nos tratamentos com MEM sozinho, 5ng/ml de progesterona + FSH ou 10ng/ml de progesterona sozinha. A análise ultra--estrutural conϐirmou a integridade follicular após 7 dias de cultivo no tratamento com 2,5ng/ml de progesterona + FSH. Em conclusão, este estudo demonstrou que a interação entre progesterona e FSH mantém a integridade ultra--estrutural, estimula a ativação de folículos primordiais e o posterior crescimento de folículos pré-antrais caprinos cultivados ...

Granulosa cell apoptosis by impairing antioxidant defense system and cellular integrity in caprine antral follicles post malathion exposure

“…Particularly, granulosa cells of an ovarian follicle have been documented to play a major role in deciding the fate of follicles by providing factors that helps in growth and maintenance of follicles or by itself undergoing apoptosis, a type of programmed cell death, that results in atresia (Matsuda et al, ). Although a number of factors contribute to apoptosis in granulosa cells resulting in unusual loss of healthy follicles directly affecting fertility of an organism (Araujo et al, ; Lima‐Verde et al, ), the role of organophosphate pesticides is important and matter of concern. Malathion too has been reported to decrease the number of healthy follicles with a significant rise in atretic follicles percentage in wister albino rats at low doses concentration (Koc et al, ).…”

Malathion-Induced Granulosa Cell Apoptosis in Caprine Antral Follicles: An Ultrastructural and Flow Cytometric Analysis

“…Follicular atresia is mediated by apoptosis of granulosa cells, a fundamental biological process of programmed cell death (Tilley et al, 1991; Yu et al, 2004), characterized by condensed cytoplasm, hazy cytosol, and pyknotic nuclei (Sharma & Bhardwaj, 2009; Escobar et al, 2013). Various factors have been reported that control follicle loss via apoptosis of granulosa cells (Yu et al, 2004; Araujo et al, 2010; Lima-Verde et al, 2012) but the role of OPs as an inducer of apoptosis is important and of much concern. OPs mediate apoptosis in granulosa cells by distinct mechanisms such as methyl parathion, which inhibits compensatory ovarian hypertrophy and increases the number of atretic follicles (Asmathbanu & Kaliwal, 1997).…”