The use of enzyme-linked immunosorbent assay and immunoblotting for the detection of Campylobacter fetus immunoglobulins in the cervico-vaginal mucus of female cattle

Pellegrin, A. O.; Miranda, Karina Leite; Figueiredo, Josely F.; Barbosa, Edel Figueiredo; Lage, Andrey Pereira

doi:10.1590/s0100-736x2011000300011

Cited by 3 publications

(4 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although, the diagnosis of BGC in herds is preferentially done in bulls, with agent identification, serological tests such as agglutination and ELISA have been used for the detection of antibodies against C. fetus subsp. venerealis in vaginal mucus samples (PELLEGRIN et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

Selection and characterization of peptides mimetic to Campylobacter fetus subsp. venerealis using phage display

et al. 2021

View full text Add to dashboard Cite

Bovine genital campylobacteriosis (BGC) is a venereal and subclinical disease that affects the fertility of cattle herds, and it is caused by Campylobacter fetus subsp. venerealis . This study selected peptides mimetic to the BGC-causing agent from a phage library. Phage display is a technique that applies bacteriophage libraries that reveal peptides fused to the viral capsid in biological selections against target proteins. Biopannings were performed for biological selection in the phage library using rabbit hyperimmune serum and C. fetus subsp. venerealis protein extract. Five selected heptapeptides were considered mimetic to Cfv-NCTC 10354 based on the results of bioinformatics analysis and assays with hyperimmune serum and cervicovaginal mucus obtained from heifers. ALASLPL and LSYLFPP were the most reactive peptides and considered promising as possible mimetic immunogens for C. fetus subsp. venerealis.

show abstract

Section: Discussionmentioning

confidence: 99%

Selection and characterization of peptides mimetic to Campylobacter fetus subsp. venerealis using phage display

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Previous studies to improve BGC diagnosis have used ELISA to detect IgA in samples such as cervical mucus, preputial washings, and contents of aborted fetuses (DEVENISH et al, 2005;HEWSON et al, 1985;MSHELIA et al, 2010;PELLEGRIN et al, 2011;HUM & QUINN, 1994). These IgA antibodies were chosen because they persist for longer and their concentration remains constant in the genital tract for many months (HUM & QUINN, 1994).…”

Section: Molecular Identification Of C Fetusmentioning

confidence: 99%

“…The practice of discarding bulls bearing C. fetus subsp. venerealis, according to PELLEGRIN et al (2002), as well as the implementation of a limited breding season (60 to 90 days), discarding of nonpregnant females at the end of the breeding season, and sexual rest for 3 to 4 cycles for recovery of females, are also measures recommended for BGC control.…”

Section: Prevention and Controlmentioning

confidence: 99%

Bovine genital campylobacteriosis: main features and perspectives for diagnosis and control

Balzan

Ziech²,

Gressler

et al. 2020

Cienc. Rural

View full text Add to dashboard Cite

Bovine genital campylobacteriosis (BGC) is a venereal disease caused by Campylobacter fetus subsp. venerealis. In countries with large cattle herds, such as Brazil, where the use of natural breeding as a reproductive strategy is a common practice, BGC is considered an important cause of reproductive failure and economic losses. In these cases, the bull is the asymptomatic carrier of the bacterium and the infected females can have infertility and even abortions. The techniques for the diagnosis of C. fetus are isolation in culture medium and identification by biochemical tests, immunofluorescence, immunoenzymatic assays and molecular techniques. Disease control is based on vaccination with bacterins. This review described the epidemiology, etiology, pathogenesis, and advances in the diagnosis and control of BGC.

show abstract

“…Economic losses due to sexually transmitted BGC are important in regions using reproduction by natural breeding (14), such as Brazil, where it is widespread (2) and highly prevalent (16). Due to the fastidiousness and restriction of the microorganism to the genital tract (5), which elicits mainly a mucosal immune response (17), routine diagnosis of BGC is based on a direct fluorescent-antibody test (7) or molecular techniques (6,14), which could lack specificity (14,20). Vaccination is the main BGC control strategy (4), but it shows low efficacy in bulls (8).…”

mentioning

confidence: 99%

Complete Genome Sequence of Type Strain Campylobacter fetus subsp. venerealis NCTC 10354T

Stynen¹,

Lage²,

Moore³

et al. 2011

Journal of Bacteriology

View full text Add to dashboard Cite

The use of enzyme-linked immunosorbent assay and immunoblotting for the detection of Campylobacter fetus immunoglobulins in the cervico-vaginal mucus of female cattle

Cited by 3 publications

References 33 publications

Selection and characterization of peptides mimetic to Campylobacter fetus subsp. venerealis using phage display

Selection and characterization of peptides mimetic to Campylobacter fetus subsp. venerealis using phage display

Bovine genital campylobacteriosis: main features and perspectives for diagnosis and control

Complete Genome Sequence of Type Strain Campylobacter fetus subsp. venerealis NCTC 10354T

Contact Info

Product

Resources

About