The number and profile of reactive NADH-d and NADPH-d neurons of myenteric plexus of six-month-old rats are different in the cecum portions

Abstract

Whole-mount preparations were prepared and submitted to NADH-diaphorase and NADPH-diaphorase histochemistry techniques. The myenteric plexus arrangement and the number of neurons were comparatively evaluated among the different portions of the cecum. The neurons from the apical and basal regions were distributed in classes at intervals of 100μm 2 , the means of the corresponding intervals being compared. The ganglia, in both techniques, were often connected by fine bundles, which became thicker in the mesenteric region and in the region next to the cecal ampulla. entre as diferentes porções das regiões do ceco. Os neurônios das regiões apical e basal foram distribuídos em classes com intervalos de 100μm 2 , sendo comparadas às médias da mensuração dos pares. Os gânglios, em ambas as técnicas, apresentavam-se, em geral, conectados por feixes delicados, tornando-se mais espessos na porção mesentérica e naquela próxima à ampola cecal. O número de neurônios NADH-d positivos foi maior do que o de NADPH-d em todas as porções, de ambas as regiões. O número de neurônios reativos a NADH-d e NADPH-d foi significativamente diferente entre as diferentes porções do ceco, com exceção das comparações entre as porções basal antimesentérica e basal intermediária, para os primeiros; e entre a basal intermediária e porção próxima à ampola cecal, e comparando-se a apical mesentérica e porção próxima à ampola cecal, para os neurônios NADPH-d positivos. A área do perfil dos neurônios NADH-d e NADPH-d reativos foi maior na região apical do que na basal. Pela primeira vez, o número de neurônios do plexo mioentérico é reportado em porções pré-estabelecidas do ceco de ratos. Nossos resultados reiteram a importância da indicação precisa da porção estudada em pesquisas envolvendo este segmento intestinal.

Effects of ascorbic acid supplementation in ileum myenteric neurons of streptozotocin-induced diabetic rats

“…In higher vertebrates, the neuron organization in the myenteric plexus forming ganglia interconnected by nerve fibers between the muscle layer strata of the gastro-intestinal segments is well described in the literature (Gabella 1979, 1990, Molinari et al 1994, Sant'Ana et al 1997, Furness 2000, Mari et al 2007, Silva et al 2008) and is not modified by diabetes (Zanoni et al 1997, 2003, Silverio et al 2008. Similarly, the plexus organization remained unchanged among the groups studied (C, CS, DS, and D), not changing either with the diabetes onset and or by the AA-supplementation.…”

“…We can justify the controversial results for the NADPH-dp neurons in Group D, C, CS and DS when compared to other reports: the diabetes maintenance period as well as the neurochemical phenotype of the stained neurons was not similar. Enhanced changes in the neuronal density can be better observed when a generalized neuronal population is stained regardless of their neurochemical phenotype, as occurs, for example, with the use techniques such as the cuprolinic blue (Phillips et al 2004), myosin V (Zanoni et al 2003), NADH-diaphorase (Sant'Ana et al 2001, Young et al 1993, Clebis et al 2004, , Zanoni et al 2007, Silva et al 2008, methylene blue (Hernandes et al 2000), among others, which stain the whole neuronal population, including the nitrergic neurons. When analyzing specific populations, such as NADPH-dp neurons, more specific results were obtained.…”

“…In higher vertebrates, the myenteric plexus is located between the longitudinal and circular strata of the muscle layer of the gastrointestinal segments forming a neuron network gathered predominantly in ganglia interconnected by bundles of nerve fibers (Gabella 1979, 1990, Molinari et al 1994, Sant'Ana et al 1997, Furness 2000, Mari et al 2007, Silva et al 2008.…”

“…Changes in neurons of the myenteric plexus have been described in different conditions and experimental models which have tried to elucidate the mechanisms that lead to functional impairment of the digestive tube (Fregonesi et al 2001, Furlan et al 2002, Zanoni et al 2003, Fontes et al 2004, Alves et al 2006, Araújo et al 2006, Silverio et al 2008, Silva et al 2008.…”