Identificação e diferenciação de herpesvírus bovino tipos 1 e 5 isolados de amostras clínicas no Centro-Sul do Brasil, Argentina e Uruguai (1987-2006)

Silva, Mariana Sá e; Brum, Mário Celso Sperotto; Weiblen, Rudi; Flores, Eduardo Furtado

doi:10.1590/s0100-736x2007001000003

Cited by 30 publications

(42 citation statements)

References 35 publications

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“…BoHV-5 and -1 are widespread among South American cattle and cause important losses to the livestock industry (Silva et al 2007b, Suarez-Heinlein et al 1993. Although BoHV-5 infection has been described in other continents, it seems to be more prevalent in South American countries like Argentina and Brazil (Silva et al 2007b).…”

Section: Introductionmentioning

confidence: 99%

Immunogenicity of an inactivated bovine herpesvirus type 5 strain defective in thymidine kinase and glycoprotein E

et al. 2010

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RESUMO.-[Imunogenicidade de uma cepa inativada do herpesvírus bovino tipo 5 defectiva na timidina quinase e glicoproteína E.] A imunogenicidade de vacina experimental inativada, produzida com uma cepa do herpesvírus bovino tipo 5 defectiva nos genes da timidina quinase e glicoproteína E (BoHV-5 gE/TKΔ) foi avaliada em bovinos e o resultado foi comparado com a resposta induzida pela cepa parental do The immunogenicity of an inactivated, experimental vaccine based on a bovine herpesvirus type 5 strain defective in thymidine kinase and glycoprotein E (BoHV-5 gE/ TKΔ) was evaluated in cattle and the results were compared with a vaccine containing the parental BoHV-5 strain (SV507/99). To formulate the vaccines, each virus (wildtype SV507/99 and BoHV-5 gE/TKΔ) was multiplied in cell culture and inactivated with binary ethyleneimine (BEI). Each vaccine dose contained approximately of 10 7.5 TCID 50 of inactivated virus mixed with an oil-based adjuvant (46:54). Forty calves, 6 to 9-monthsold, were allocated into two groups of 20 animals each and vaccinated twice (days 0 and 22pv) by the subcutaneous route with either vaccine. Serum samples collected at day 0 and at different intervals after vaccination were tested for virus neutralizing (VN) antibodies against the parental virus and against heterologous BoHV-5 and BoHV-1 isolates. The VN assays demonstrated seroconversion to the respective homologous viruses in all vaccinated animals after the second vaccine dose (mean titers of 17.5 for the wildtype vaccine; 24.1 for the recombinant virus). All animals remained reagents up to day 116 pv, yet showing a gradual reduction in VN titers. Animals from both vaccine groups reacted in similar VN titers to different BoHV-1 and BoHV-5 isolates, yet the magnitude of serological response of both groups was higher against BoHV-5 field isolates. Calves vaccinated with the recombinant virus did not develop antibodies to gE as verified by negative results in a gE-specific ELISA, what would allow serological differentiation from naturally infected animals. Taken together, these results indicate that inactivated antigens of BoHV-5 gE/TK recombinant virus induced an adequate serological response against BoHV-5 and BoHV-1 and thus can be used as an alternative, differential vaccine candidate. inativados emulsionado em adjuvante oleoso (46:54). Quarenta bezerros de raças cruzadas, com idade entre seis a nove meses, foram alocados em dois grupos de 20 animais cada e vacinados duas vezes (dia 0 e 22 pv) pela via subcutânea com uma das vacinas. Amostras de soro foram coletadas no dia 0 e a vários intervalos após vacinação para a pesquisa de anticorpos neutralizantes frente ao vírus homólogo ou frente a isolados de BoHV-5 e BoHV-1. Os testes de soroneutralização (SN) demonstraram que todos os animais soroconverteram após a segunda dose da vacina (títulos médios de 17,5 para o grupo SV507/99; 24,1 para o grupo BoHV-5 gE/TKΔ). Todos os animais mantiveram níveis de anticorpos neutralizantes até o dia 116 pv, no entanto foi observada uma redução ...

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Section: Introductionmentioning

confidence: 99%

Immunogenicity of an inactivated bovine herpesvirus type 5 strain defective in thymidine kinase and glycoprotein E

et al. 2010

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“…Our collaborators first reported the construction of a gE-deleted recombinant BoHV-1 intended to be used as a vaccine strain (Franco et al 2002). As BoHV-1 and BoHV-5 co-circulate in Brazilian cattle (Silva et al 2007), a commercial vaccine for use in the country would require the inclusion of both viruses. To this end, we constructed a double deletion BoHV-5 gE-and tk-mutant (BoHV-5gEΔTKΔ, Brum et al 2010a).…”

Section: Discussionmentioning

confidence: 99%

“…Cells were grown on minimal essential medium (MEM), containing ampicillin (1.6mg/L), streptomycin (0.4mg/L), amphotericin (2mg/L), supplemented with 10% fetal bovine serum (Cultilab, Brazil). The virus used for gE cloning was the BoHV-1.2 isolate SV56/90 (Weiblen et al 1992, Silva et al 2007). The viruses used in the characterization of the recombinant proteins were Brazilian BoHV-1 (SV-265 and 265gE-) and BoHV-5 (SV507/99) strains described elsewhere (Franco et al 2002, Silva et al 2007.…”

Section: Cells Virus and Bacterial Strainsmentioning

confidence: 99%

“…The virus used for gE cloning was the BoHV-1.2 isolate SV56/90 (Weiblen et al 1992, Silva et al 2007). The viruses used in the characterization of the recombinant proteins were Brazilian BoHV-1 (SV-265 and 265gE-) and BoHV-5 (SV507/99) strains described elsewhere (Franco et al 2002, Silva et al 2007. Escherichia coli strain BL21 (DE3) was used for initial cloning, sequencing and maintenance of DNA fragment.…”

Section: Cells Virus and Bacterial Strainsmentioning

confidence: 99%

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Prokaryotic expression of a truncated form of bovine herpesvirus 1 glycoprotein E (gE) and its use in an ELISA for gE antibodies

et al. 2013

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Pesq. Vet. Bras. 33(1): 41-46, janeiro 2013 41 RESUMO.-[Expressão procariota de uma forma truncada da glicoproteína E (gE) do herpesvírus bovino tipo 1 e uso em ELISA para anticorpos contra a gE.] Este trabalho relata a expressão de uma forma truncada da glicoproteína E (gE) do herpesvírus bovino tipo 1 (BoHV-1) para uso em imunodiagnóstico. Um fragmento de 651 pares de bases (pb) correspondente ao terço amino-terminal (217 aminoácidos) da gE do BoHV-1 -que compartilha uma alta identidade com a gE do BoHV-5 -foi clonada como proteína de fusão com cauda 6x de histidina em um vetor de expres- This article describes the expression of a truncated form of bovine herpesvirus 1 (BoHV-1) glycoprotein E (gE) for use as immunodiagnostic reagent. A 651 nucleotide fragment corresponding to the amino-terminal third (217 amino acids) of BoHV-1 gE -that shares a high identity with the homologous BoHV-5 counterpart -was cloned as a 6×His-tag fusion protein in an Escherichia coli expression vector. A soluble protein of approximately 25 kDa purified from lysates of transformed E. coli was recognized in Western blot (WB) by anti-6xHis-tag and anti-BoHV-1 gE monoclonal antibodies. In addition, the recombinant protein was specifically recognized in WB by antibodies present in the sera of cattle seropositive to BoHV-1 and BoHV-5. An indirect ELISA using the expressed protein as coating antigen performed comparably to a commercial anti-gE ELISA and was able to differentiate serologically calves vaccinated with a gE-deleted BoHV-5 strain from calves infected with BoHV-1. Thus, the truncated gE may be useful for serological tests designed to differentiate BoHV-1/BoHV-5 infected animals from those vaccinated with gE-negative marker vaccines. bovine herpesvirus, vaccine, DIVA, recombinant protein. são em Escherichia coli. Uma proteína solúvel de aproximadamente 25 kDa purificada de lisados de E.coli foi reconhecida em Western blot (WB) por anticorpos monoclonais anti-6xHis-tag e anti-gE. Além disso, a proteína recombinante purificada foi reconhecida em WB por anticorpos presentes no soro de animais soropositivos ao BoHV-1 e BoHV-5. Um ELISA indireto utilizando a proteína recombinante como antígeno apresentou performance comparável a um ELISA gE comercial e foi capaz e diferenciar sorologicamente animais vacinados com uma cepa gE-negativa de BoHV-5 de animais infectados com o BoHV-1. Portanto, a gE truncada pode ser útil em testes sorológicos diferenciais para uso conjunto com vacinas com marcador antigênico gE para o BoHV-1 e BoHV-5.TERMOS DE INDEXAÇÃO: BoHV-5, herpesvírus bovino, vacina diferencial, proteína recombinante.

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“…Bovine herpesvirus 1 has been associated with respiratory tract infections (bovine infectious rinotracheitis, IBR), abortions, sporadic cases of encephalitis and reproductive tract infections (vulvovaginitis or balanopostitis) (Engels and Ackermann 1996, Muylkens et al 2007, Nandi et al 2009. Bovine herpesvirus 5 seems to be widely distributed in Latin America (Esteves et al 2008, Pidone et al 1999, Silva et al 2007, although it has been occasionally found in other regions of the world (Bartha et al 1969, Carrillo et al 1983, Diallo et al 2010, d'Offay et al 1993, Eugster et al 1975, Kirkland et al 2009. Bovine herpesvirus 5 has been associated with encephalitis and meningoencephalitis in calves and is of utmost importance in the differential diagnosis of bovine spongiform encephalitis .…”

Section: Introductionmentioning

confidence: 99%

Detection of bovine herpesvirus 1 and 5 in trigeminal ganglia of beef cattle in Uruguay

Furtado¹,

Campos

Llambí³

et al. 2014

Arch. med. vet.

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Identificação e diferenciação de herpesvírus bovino tipos 1 e 5 isolados de amostras clínicas no Centro-Sul do Brasil, Argentina e Uruguai (1987-2006)

Cited by 30 publications

References 35 publications

Immunogenicity of an inactivated bovine herpesvirus type 5 strain defective in thymidine kinase and glycoprotein E

Immunogenicity of an inactivated bovine herpesvirus type 5 strain defective in thymidine kinase and glycoprotein E

Prokaryotic expression of a truncated form of bovine herpesvirus 1 glycoprotein E (gE) and its use in an ELISA for gE antibodies

Detection of bovine herpesvirus 1 and 5 in trigeminal ganglia of beef cattle in Uruguay

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