Serological diagnosis of visceral leishmaniasis by an enzyme immunoassay using protein A in naturally infected dogs

Lima, Valéria Marçal Felix de; Biazzono, Luciane; Silva, Ana Cláudia; Corrêa, Ana Paula Ferreira Lopes; Luvizotto, Maria Cecília Rui

doi:10.1590/s0100-736x2005000400005

Cited by 36 publications

(32 citation statements)

References 17 publications

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“…A maior reatividade da proteína A (média geral de DO 0,546) no ELISA indireto pode ser explicada por sua alta afinidade pelos anticorpos da classe IgG presentes no soro em resposta específica à infecção (Lima et al 2005). A Proteína A pode reagir tanto com as subclasses IgG1, IgG2a, IgG2b e IgG3, se ligando à cadeia pesada do anticorpo, quanto com os anticorpos das classes IgM e IgA caninas (Goudswaard et al 1978, Lima et al 2005.…”

Section: Discussionunclassified

“…A Proteína A pode reagir tanto com as subclasses IgG1, IgG2a, IgG2b e IgG3, se ligando à cadeia pesada do anticorpo, quanto com os anticorpos das classes IgM e IgA caninas (Goudswaard et al 1978, Lima et al 2005. Dessa forma, animais na fase aguda da infecção, ainda não detectáveis em testes de triagem com métodos baseados no uso de anti-IgG de cão doméstico, podem ser identificados no ensaio padronizado com a Proteína A, que ainda parece detectar uma maior abrangência de moléculas de IgGs.…”

Section: Discussionunclassified

“…adsorvidos na fase sólida . Esta proteína possui uma boa afinidade com IgG de diferentes espécies de mamíferos silvestres e exóticos (Kelly et al 1993, Stöbel et al 2002, Solano-Gallego et al 2003, Lima et al 2005.…”

Section: Introductionunclassified

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Teste de ELISA indireto para diagnóstico sorológico de leishmaniose visceral em canídeos silvestres

et al. 2013

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In South America, some wild canids are considered natural reservoirs of Leishmania chagasi. The immunological response of wild canids to Leishmania is not well understood, and the development of diagnostic methods is necessary for such purpose. In the present study, the standardization of an enzyme-linked immunosorbent assay (ELISA) for the serodiagnosis of visceral leishmaniasis (VL) in Brazilian species of wild canids is described. Serum and plasma samples from 12 captive wild canids were studied: seven from maned wolves (Chrysocyon brachyurus), three from hoary foxes (Lycalopex vetulus), and two from crab-eating foxes (Cerdocyon thous). Samples from C. brachyurus and L. vetulus, both captive in an endemic area for VL, presenting clinical disease and positivity in Indirect Immunofluorescence Reaction and Polymerase Chain Reaction tests were used as positive controls. The antibody anti-dog IgG and Protein A, both conjugated with horseradish peroxidase, were compared in indirect ELISA tests which detected four (04/12) and three (03/12) seropositive C. brachyurus for anti-Leishmania antibodies, respectively. The ELISA tests were able to clearly distinguish negative from positive samples, as the mean optical density (OD) of the negative samples was 4.8 and 15.5 times lower than those of the positive ones either using anti-dog IgG and Protein A, respectively. Samples from three ELISA -positive C. brachyurus were analyzed by Western blotting and identified immunodominant bands of 19, 22, 24, 45 and 66 kDa, among 22 protein bands detected. The ELISAs with protein A and anti-dog IgG showed respectively excellent (Kappa = 1.0; p<0.001) and moderate (Kappa = 0.8; p<0.0015) agreement with the Western blotting assay. The ELISA tests showed to be adequate for screening studies to identify antibody responses, thus indicating contact with Leishmania infection by wild canids.INDEX TERMS: Wild canids, Leishmania, visceral leishmaniasis, ELISA, protein A.

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Teste de ELISA indireto para diagnóstico sorológico de leishmaniose visceral em canídeos silvestres

et al. 2013

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“…Serum samples were selected using archived material (NUNES et al, 2008) kept at -80 °C. Serological evaluation was performed by indirect ELISA as described by Lima et al (2005) and by a quick immunochromatographic assay based on Dual-Path Platform technology (DPP®CVL rapid test, Fiocruz/Bio-Manguinhos Unit, Brazil). DNA was extracted from total blood samples using the phenol/chloroform method and PCR was performed using specific Leishmania primers for kDNA amplification (120bp), as previously described (NUNES et al, 2007).…”

Section: Sampling and Cvl Diagnosismentioning

confidence: 99%

Serological, parasitological and molecular tests for canine visceral leishmaniosis diagnosis in a longitudinal study

Nunes

Lima

Melo

et al. 2015

Rev. Bras. Parasitol. Vet.

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One of the measures to control visceral leishmaniosis (VL) in Brazil is the identification and culling of the canine reservoir. There is much controversy concerning this strategy, including the proper identification of positive dogs and the fact that the host-parasite relationship changes over time make it more challenging. A dynamic cohort of 62 dogs was followed every three months using serological and parasitological examinations and PCR. Positivity by PCR was higher than by serology and by parasitological examinations and showed a tendency to decrease over time, while serology tended to increase after six months. Concomitant positivity in all tests was observed in 10.4% of the samples, and negativity in 29.1%. Overall sensitivity ranged from 43.6 to 64.1%, and was not uniform over time. The proportion of dogs with or without clinical signs was not different by cytology or PCR but PCR was able to identify a larger number of asymptomatic dogs compared to ELISA and immunochromatography. PCR can be useful for surveillance of areas where cases of canine VL have not yet been detected and in which control strategies can be implemented to limit the spread of the disease. Despite the advance in diagnostic tools CVL diagnosis remains a challenge.

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“…ELISA (Enzyme Linked Immunosorbent Assay) represents a simple and rapid method for the survey of this canine infection (Lima et al, 2005), allowing the processing of a considerable number of samples in a short time interval (Maia & Campino, 2008).…”

Section: Diagnosismentioning

confidence: 99%

Endoparasites with Zoonotic Potential in Domesticated Dogs

Bresciani¹,

Coelho²,

Gomes³

et al. 2012

Zoonosis

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Serological diagnosis of visceral leishmaniasis by an enzyme immunoassay using protein A in naturally infected dogs

Cited by 36 publications

References 17 publications

Teste de ELISA indireto para diagnóstico sorológico de leishmaniose visceral em canídeos silvestres

Teste de ELISA indireto para diagnóstico sorológico de leishmaniose visceral em canídeos silvestres

Serological, parasitological and molecular tests for canine visceral leishmaniosis diagnosis in a longitudinal study

Endoparasites with Zoonotic Potential in Domesticated Dogs

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