Estudo clínico e características do suco ruminal de caprinos com acidose láctica induzida experimentalmente

Neto, E. G. Miranda; Afonso, José Augusto Bastos; Mendonça, Carla Lopes de; Almeida, Mayra Zilta P.R.B. de

doi:10.1590/s0100-736x2005000200002

Cited by 18 publications

(18 citation statements)

References 14 publications

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“…A slight predominance of small protozoa was observed in the pasture group (39.9±13.1%) and pasture and concentrate group (34.7±3.6%), followed by medium-sized protozoa (32.2±4.1% and32.7±1.8%, respectively) and largesized protozoa (27.9±17.0% and 32.7±1.8%, respectively); these results are consistent with those of Barbosa et al (2003) andMiranda Neto et al (2005). During storage, the small protozoa were the first to lose viability in both groups, while the large protozoa remained viable for a longer period.…”

Section: Resultssupporting

confidence: 87%

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Influence of food, storage temperature, and time on the extracorporeal viability of ruminal fluid of cattle

Martin

Hilgert

Guirro

2018

SCA

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Rumen fluid obtained from slaughter houses is sometimes used for transfaunation. In this study, we evaluated the ruminal fluid of cattle fed on pasture or pasture and concentrate and slaughtered recently. The rumen fluid samples were stored in a water bath, at ambient temperature, cooled, or frozen and subjected to physical, chemical, and microbiological evaluation for 24 h. The color, consistency, and odor changed primarily in the cooled samples, followed by the ambient temperature and water bath samples. The pH of fresh ruminal fluid was 7.5 in the pasture group and 6.4 in the pasture and concentrate group. The methylene blue reduction time of the fresh ruminal fluid was 2.35 min in the pasture group and 1.86 min in the pasture and concentrate group; the best values were observed in the water bath group. The chloride content was 15.7 mEq/l in the pasture group and 16.3±3.6 mEq/l in the pasture and concentrate group. A predominance of gram-negative bacteria was observed. The concentration of protozoa was 51.5 in the pasture group and 47.5 × 10 4 /ml in the pasture and concentrate group, with a slight predominance of small protozoa; motility was better in the water bath samples than in the ambient temperature, cooled, and frozen samples. The viability of ruminal fluid collected from freshly slaughtered cattle was influenced by food provided in vivo; however, changes as a function of time and storage temperature were more remarkable. The rumen fluid was viable for up to 9 h when stored in the water bath (38°C) and 2 h at ambient temperature, and it was observed to be nonviable when subjected to cooling or freezing. Key words: Microbiota. Rumen. Ruminal fluid. Storage. Transfaunation. ResumoAlgumas vezes é necessário utilizar fluido ruminal obtido em abatedouros para transfaunação. Este estudo avaliou o fluido ruminal de bovinos recém abatidos alimentados in vivo com pasto ou pasto e concentrado estocados sob banho-maria, temperatura ambiente, refrigerado ou congelado submetidos à análise física, química e microbiológica por 24 horas. A cor, consistência e odor se alteraram primeiramente em refrigeração, seguido por temperatura ambiente e banho-maria. A fresco (TF) o pH foi de 7,5 no grupo pasto e 6,4 no grupo pasto e concentrado. O tempo de redução do azul de metileno a fresco foi 2.35 min no grupo pasto e 1.86 no grupo que recebeu pasto e concentrado, os melhores valores foram encontrados no grupo banho-maria. A concentração de cloreto no grupo pasto foi 15,7 Martin, C. C.; Hilgert, A. R.; Guirro, E. C. B. do P.e no grupo pasto e concentrado foi 16,3 mEq/L. Em relação às bactérias houve predomínio de Gram negativas. A concentração de protozoários foi 51,5 no grupo pasto e 47,5x10 4 /ml no grupo pasto e concentrado com ligeira dominância de protozoários pequenos; a motilidade foi melhor em banhomaria que em temperatura ambiente, refrigerado ou congelado, respectivamente. A viabilidade do fluido ruminal coletado de bovinos recém abatidos é influenciada pela alimentação in vivo, mas as alterações decorrente...

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Section: Resultssupporting

confidence: 87%

“…However, ruminants that receive a high-carbohydrate diet can show reduction in pH, resulting in the proliferation of Streptococcus bovis and Lactobacillus sp. that are tolerant to low pH and, thus, predominance of gram-negative bacteria (MIRANDA NETO et al, 2005;MOREIRA et al, 2009). …”

Section: Resultsmentioning

confidence: 99%

Influence of food, storage temperature, and time on the extracorporeal viability of ruminal fluid of cattle

Martin

Hilgert

Guirro

2018

SCA

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“…The clinical observations in the goats of the two groups in this study was characteristic of RLA and the intensity of the clinical manifestations coincided with the decrease in ruminal pH, corroborating with other authors who evaluated the disease in this species under experimental conditions (CAO et al, 1987;MIRANDA NETO et al, 2005) and cases of natural occurrence (JOSHI et al, 2017). The majority of the animals in the two groups presented clinical reestablishment 48h after induction, differing from other authors who observed in sheep experimentally submitted to ruminal acidosis, early recovery in animals receiving monensin sodium in the diet, when compared to the control group, allowing the authors to infer that supplementation with ionophore contributed to faster recovery of the ruminal environment, reducing the severity of the clinical presentation (AFONSO et al, 2005;REIS et al, 2018a).…”

Section: Discussionsupporting

confidence: 91%

“…Alterations in the physical characteristics of the ruminal fluid observed in the two groups, such as milky color, acid odor, and aqueous consistency, are related to the accumulation of organic acids and consequent increase in ruminal osmolarity (CAO et al, 1987) and were similar to those described in natural cases of the disease by Joshi et al (2017) and under experimental conditions by Miranda Neto et al (2005), who also observed the beginning of these alterations from 4h PI and reestablishment of these characteristics concomitantly with the elevation in the ruminal pH and the clinical recovery of the animals. The significant reduction in ruminal pH observed in both groups from 4h after induction of the fermentative disorder was similar to that observed in other models when different doses of sucrose and animal species were used (CAO et al, 1987;HAJI HAJIKOLAEI et al, 2006;MIRANDA NETO et al, 2005). These authors reported ruminal pH values lower than 5.0 between 12h and 36h after induction of RLA and reestablishment of this variable from 48h.…”

Section: Discussionsupporting

confidence: 65%

Cardiac biomarkers in goats with experimental ruminal lactic acidosis and supplemented with monensin sodium

Ribeiro¹,

Soares²,

Silva³

et al. 2020

Rev Agr Acad

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Aimed to evaluate creatine kinase MB (CK-MB) and cardiac troponin I (cTnI) in goats with experimental ruminal lactic acidosis (RLA) and supplemented with monensin sodium (MS). Twenty goats were divided into control group (CG) and treatment group (TG) supplemented with 33mg/animal/day of MS. The RLA was induced (10g/Kg body weight of sucrose) and before and post-induction clinical data, ruminal fluid and blood were collected. All animals presented mild RLA, with minimal ruminal pH at 8h post-induction. CK-MB and cTnI were higher in the CG and TG, respectively, without differ between moments. MS at the dose used did not prevent RLA whose severity was not sufficient to cause cardiac injury.

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“…Casos de acidose láctica ruminal que ocorrem naturalmente estão associados à ingestão não intencional de grãos em grandes quantidades, com influência do seu tipo e do processamento, além de tubérculos ou frutas com altas quantidades de amido ou açúcares, soro de leite e outros, ou também ao aumento do consumo de rações concentradas, ricas em carboidratos de rápida fermentação visando ao maior desempenho produtivo do animal (Miranda Neto et al, 2005;Nagaraja e Lechtenberg, 2007).…”

Section: Introductionunclassified

Avaliação clínica de ovinos após a ingestão de elevada quantidade de manga

Soares

Lima

Oliveira

et al. 2017

Arq. Bras. Med. Vet. Zootec.

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 RESUMOObjetivou-se avaliar clinicamente os efeitos da administração intrarruminal de duas quantidades distintas de manga em ovinos. Foram utilizados sete ovinos machos, hígidos, que não receberam carboidratos não fibrosos por, pelo menos, seis meses previamente ao período experimental, quando se avaliou pH ruminal, total de protozoários no suco de rúmen, pH urinário, pH sanguíneo estimado e parâmetros vitais nos tempos zero, 12, 16, 20 e 24 após a administração da manga. Os sete ovinos foram distribuídos aleatoriamente em dois grupos e receberam 0,625% ou 1,875% da MS de manga/kg/PV (M1 e M2, respectivamente), distribuídos em delineamento cross-over, com uma parcela perdida, e 30 dias de washout. Os ovinos M2 apresentaram valores médios para pH ruminal significativamente inferiores a M1 a partir do tempo T12 (5,1 e 6,9, respectivamente), o que indica ocorrência de acidose ruminal. Os animais tratados com M1 não apresentaram alterações sistêmicas, ao passo que os ovinos tratados com M2 apresentaram acidose metabólica leve, detectada por meio do pH urinário ácido (4,8). A ingestão apenas de manga in natura na quantidade de 1,875% da MS de manga/kg/PV mostrou-se capaz de provocar acidose ruminal em ovinos, levando-os a: ligeira depressão, aumento da frequência cardíaca, diminuição dos movimentos ruminais e diarreia em alguns casos.Palavras-chave: acidose, rúmen, manga, pequenos ruminantes ABSTRACT The aim of this study is to clinically evaluate the effects of intra ruminal administration of two different

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Estudo clínico e características do suco ruminal de caprinos com acidose láctica induzida experimentalmente

Cited by 18 publications

References 14 publications

Influence of food, storage temperature, and time on the extracorporeal viability of ruminal fluid of cattle

Influence of food, storage temperature, and time on the extracorporeal viability of ruminal fluid of cattle

Cardiac biomarkers in goats with experimental ruminal lactic acidosis and supplemented with monensin sodium

Avaliação clínica de ovinos após a ingestão de elevada quantidade de manga

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