Caracterização de herpesvírus bovinos tipos 1 (BHV-1) e 5 (BHV-5) com anticorpos monoclonais

Souza, Vanessa Felipe; Melo, Silvia Valim; Esteves, Paulo Augusto; Schmidt, Candice; Gonçalves, David; Schaefer, Rejane; Silva, T. C.; Almeida, Renata Servan de; Vicentini, Franco Kindlein; Franco, Ana Cláudia; Oliveira, Elsa; Spilki, Fernando Rosado; Weiblen, Rudi; Flores, Eduardo Furtado; Lemos, Ricardo A.A.; Alfieri, Amauri Alcindo; Pituco, Edviges Maristela; Roehe, P. M.

doi:10.1590/s0100-736x2002000100004

Cited by 44 publications

(41 citation statements)

References 20 publications

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“…In contrast, the carboxy-terminal two thirds of these glycoproteins showed a high (90.0%) homology (10). The high degree of homology in this region explains the cross-reactivity of a number of BHV-1 and BHV-5 glycoprotein C mAbs and may contribute to the extensive cross-neutralization observed between these viruses (10)(11)(12)16,20,27). The moderate to low antigenic diversity of other envelope glycoproteins involved in virus neutralization by antibodies (namely glycoprotein B and glycoprotein D) may also contribute to the high cross-neutralization between BHV-1 and BHV-5 (11,13,25).…”

Section: Discussionmentioning

confidence: 98%

“…Although a number of BHV-1 and BHV-5 glycoprotein C mAbs have been shown to cross-react, some mAbs with the ability to distinguish between BHV-1 and BHV-5 (as shown for mAbs 1F3 and 2F9) have also been reported (11,12,20). Nucleotide sequencing and epitope mapping have shown that most of BHV-1 and BHV-5 glycoprotein C-specific epitopes are located within the amino-terminal region of the protein (10).…”

Section: Discussionmentioning

confidence: 99%

“…The virus was biologically cloned three times prior to production of viral stocks for antigen production. The BHV-1 and BHV-5 isolates used to test the spectrum of reactivity of the mAbs have been previously characterized (20). The double gene-deleted BHV-1 mutant (IBRVdlTKdlgIII) and the parental BHV-1 Los Angeles strain used in the characterization of mAbs have been previously described (23).…”

Section: Cells and Virusesmentioning

confidence: 99%

“…Likewise, extensive serological cross-reactivity has been demonstrated between BHV-1 and BHV-5 (17)(18)(19). Nevertheless, the occurrence of cross-protection between BHV-1 and BHV-5 is still controversial (8,17,(19)(20)(21)(22). A possible cross-protection between these viruses would be of obvious interest for immunization strategies and vaccine production since only BHV-1 vaccines are available to date (21).…”

Section: Introductionmentioning

confidence: 99%

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Production and characterization of monoclonal antibodies to a Brazilian bovine herpesvirus type 5

Oldoni

Weiblen

et al. 2004

Braz J Med Biol Res

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Antigens of a bovine herpesvirus type 5 (BHV-5), isolated from a cow with a neurological infection in Rio Grande do Sul State, Brazil, were used to immunize BALB/c mice to produce monoclonal antibodies (mAbs). Eleven hybridomas secreting mAbs directed at BHV-5 antigens were obtained after two fusions and screening of 356 hypoxanthine-aminopterin-thymidine-resistant clones. The mAbs reacted at dilutions up to 1:500 (hybridoma culture supernatant) and up to >1:10,000 (ascitic fluid) in an indirect fluorescent antibody assay (IFA) and in immunoperoxidase staining of BHV-5-infected cells. Four mAbs (1D12, 2E2, 2G10 and 4E4) showed virus-neutralizing activity against the parental BHV-5 isolate. Five mAbs (1F3, 2A6, 2F9, 2G10 and HB24L) reacted in Western immunoblotting with a protein of approximately 90 kDa. Three other mAbs (2E2, 3D6 and 4E4) reacted in IFA with antigens of a BHV-1 mutant glycoprotein Cnegative strain, demonstrating that they are directed at a viral antigen other than glycoprotein C. The eleven mAbs tested reacted with 20 BHV-5 field isolates and nine mAbs reacted with 10 BHV-1 isolates. Two mAbs (1F3 and 2F9) failed to react with BHV-1 field isolates, although they displayed a weak and nonreproducible reaction with the BHV-1 reference strain Los Angeles. These mAbs may be very useful in distinguishing between BHV-1 and BHV-5 infections since most of the traditional reagents and techniques are unable to do so. One mAb (2F9) was shown to bind to viral antigens by immunohistochemistry of histological sections of the brain of a BHV-5-infected calf. These results demonstrate that the mAbs produced here are suitable for use in a variety of immunological techniques and therefore may be useful for diagnostic and research purposes.

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Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

Section: Cells and Virusesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Production and characterization of monoclonal antibodies to a Brazilian bovine herpesvirus type 5

Oldoni

Weiblen

et al. 2004

Braz J Med Biol Res

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“…The BHV-5 isolate (named EVI 340/96) was tested with a panel of monoclonal antibodies capable of distinguishing BHV-1 from BHV-5 in immunoperoxidase monolayer assays as described (Silva et al 1996, Roehe et al 1997, Almeida et al 1998). Viral DNA was extracted from the virus isolate, as well as from control BHV-5 strains (V175 and N569; Souza et al 2002) as described by Van Oirschot et al (1995) and submitted to restriction endonuclease analysis (REA; Engels et al 1986). Restriction enzyme digestion with BstEII was performed under the conditions recommended by the manufacturer (BRL) and the DNA fragments separated by electrophoresis on a 0.5 per cent agarose gel.…”

Section: Methodsmentioning

confidence: 99%

Bovine herpesvirus type 5 (BHV-5) in a calf with rabies

Spilki¹,

Franco²,

Teixeira³

et al. 2003

Pesq. Vet. Bras.

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-[Herpesvírus bovino tipo 5 (BHV-5) em bovino infectado pelo vírus da raiva. ] O encéfalo de um bezerro macho, de um ano de idade, que morreu com sinais de doença neurológica, foi submetido ao laboratório para diagnósti-co de raiva. O exame histopatológico revelou mielite moderada, meningoencefalite leve com infiltração perivascular e corpúsculos de Negri nas células de Purkinje do cerebelo. A infecção pelo vírus rábico foi ainda confirmada por imunofluorescência direta e por inoculação em camundongos. Além disso, um herpesvírus foi isolado dos tecidos do encéfalo. O vírus isolado foi antigênica e geneticamente caracterizado como Herpesvírus bovino tipo 5 (BHV-5). Não foi possível determinar se o BHV-5 teve algum papel ativo no desfecho da enfermidade, uma vez que o vírus poderia estar presente em forma latente nos tecidos neurais. Este é o primeiro relato de uma infecção mista pelo vírus da raiva e BHV-5 em bovinos.TERMOS DE INDEXAÇÃO: Herpesvírus bovino tipo 5, BHV-5, raiva.

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Molecular and antigenic characterization of bovine herpesvirus type 1 (BoHV-1) strains from cattle with diverse clinical cases in Turkey

Dağalp

Farzani

Doğan

et al. 2019

Trop Anim Health Prod

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The present study reports the molecular and antigenic characterization of 13 bovine herpesvirus type 1 (BoHV-1) field viruses obtained from cattle with different clinical cases in Turkey between 1992 and 2017. We selected glycoprotein C (gC) of BoHV-1 as a target to detect and/or verify presence of the virus in suspect materials followed by virus isolation (VI) in MDBK cells. In seven out of 13 BoHV-1 positive samples, cytophatic effects (CPEs) were observed in MDBK cell cultures, although only four virus samples reached a sufficient titer to use in phylogenetic assay, restriction endonuclease analysis (REA), and virus neutralization test (VNT). According to the results of sequence analysis of the 13 BoHV-1 positive samples, nine BoHV-1 field viruses were determined as BoHV-1.1 and four as BoHV-1.2. Using REA, we demonstrated that two of our isolated viruses could be categorized as BoHV-1.1 while the other two isolates were BoHV-1.2 subtypes. Differences between the BoHV-1.1 and BoHV-1.2 isolates were also detected in the VNT results by assaying 125 suspected serum samples after testing with isolated (KY748023, KY748022, KY748020, and KY748021) and reference viruses (BoHV-1 Cooper and BoHV-5 Texas 89). These results are indicating the need to correctly identify BoHV-1 field isolates to better understand the epidemiology and pathogenesis of infection. In addition, it would be useful to identify the subtypes circulating in the specific geographical area while determining vaccination preferences.

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Caracterização de herpesvírus bovinos tipos 1 (BHV-1) e 5 (BHV-5) com anticorpos monoclonais

Cited by 44 publications

References 20 publications

Production and characterization of monoclonal antibodies to a Brazilian bovine herpesvirus type 5

Production and characterization of monoclonal antibodies to a Brazilian bovine herpesvirus type 5

Bovine herpesvirus type 5 (BHV-5) in a calf with rabies

Molecular and antigenic characterization of bovine herpesvirus type 1 (BoHV-1) strains from cattle with diverse clinical cases in Turkey

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