Modificação da microbiota associada às lesões peridentárias da "cara inchada" em bezerros transferidos para área indene

Dutra, Iveraldo S.; Botteon, Rita C.M.; Döbereiner, Jürgen

doi:10.1590/s0100-736x2000000200003

Cited by 17 publications

(17 citation statements)

References 3 publications

(4 reference statements)

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“…Despite of scarce data regarding microbiological aspects of this disease, it was observed through culture method, that the black-pigment species constitute 80% of subgingival microflora in diseased calves (Botteon et al 1993, although distribution of different species has not been widely evaluated yet. Dutra et al (2000) observed that while periodontal pockets in calves with periodontitis contained black-pigment average percentage of 71.3% of total microflora cultivated in anaerobic conditions and specific culture method, the same animals showed only 1.7% after the vanishing of inflammation clinical symptomatology, suggesting that these microorganisms can be more prevailing and abundant during the most active disease period, by the time conjunctive attachment loss occurs, as observed in humans.…”

Section: Discussionmentioning

confidence: 89%

Presence of Porphyromonas and Prevotella species in the oral microflora of cattle with periodontitis

et al. 2015

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Abstratc: Bovine periodontitis is a progressive purulent infectious process associated with the presence of strictly and facultative anaerobic subgingival biofilm and epidemiologically related to soil management in large geographic areas of Brazil. This study aimed to detect species of the genera Porphyromonas and Prevotella, which occurr in periodontal pockets of cattle with lesions deeper than 5mm (n=26) and in gingival sulcus of animals considered periodontally healthy (n=25). Presence of the microorganisms was evaluated by independent-culture medium diagnostic method, using polymerase chain reaction (PCR) with specific primers of Porphyromonas asaccharolytica, P. endodontalis, P. gingivalis, P. gulae, Prevotella buccae, P. intermedia, P. loescheii, P. melaninogenica, P. nigrescens, P. oralis and P. tannerae. The species P. endodontalis (80.7%), P. melaninogenica (73.1%) and P. intermedia (61.5%) were the most predominant in samples of cattle with periodontitis. Regarding non-injured gingival sulcus of cattle, P. endodontalis (40%) and P. loeschei (40%) prevailed. Porphyromonas gingivalis, P. gulae and Prevotella tannerae were not detected in the 51 samples studied. Data evaluation by T test, enabled to verify that ocorrence of Porphyromonas asaccharolytica (p=0.000003), P. endodontalis (p=0.0023), Prevotella buccae (p=0.0017), P. intermedia (p=0.0020), P. melaninogenica (p=0.00006) and P. oralis (p=0.0028) is correlated with bovine periodontitis.

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Section: Discussionmentioning

confidence: 89%

Presence of Porphyromonas and Prevotella species in the oral microflora of cattle with periodontitis

et al. 2015

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“…Progression of gingivitis in periodontitis is associated with individual, environmental, and etiological factors (Lang et al 2009). It is known that pasture reform, through liming and fertilization, can favor the occurrence of bovine periodontitis in previously endemic areas (Dutra et al 2000). However, it is unknown whether this frequent association is valid for all periodontal diseases; it should be added that…”

Section: Discussionmentioning

confidence: 99%

“…Thereby, it can be evidenced that bovine oral microbiota is rich and diversified, composed of 395 genera or higher taxa and that microorganisms considered as potential periodontal pathogens are associated with the occurrence of periodontitis in these animals. These include several species of Porphyromonas, Prevotella, and Treponema, as well as Fusobacterium nucleatum, Fusobacterium necrophorum, and Actinomyces naeslundii (Dutra et al 2000, Borsanelli et al 2015a, 2015b, Borsanelli 2017.…”

Section: Introductionmentioning

confidence: 99%

Efficacy of virginiamycin for the control of periodontal disease in calves

et al. 2019

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Periodontal diseases are multifactorial infectious processes caused by complexes of microorganisms, with damage to health, production, and animal welfare. The aim of the present study was to evaluate the efficacy of virginiamycin in the prevention and control of two early forms of periodontal disease: gingivitis and necrotizing gingivitis. Ten weaned calves, aged four to six months, were permanently kept in a single lot under the same rotational grazing regime in a newly reformed area of Panicum maximum. Five of the calves were orally administered 340mg of virginiamycin (Virginiamycin Group) daily for a period of 18 weeks, while the remaining five calves (Control Group) remained under the same food management but did not receive virginiamycin. During this period, animals underwent 18 weekly evaluations regarding periodontal health, with monitoring and recording of clinical parameters of the eight deciduous incisor teeth on the labial and lingual faces. At approximately two-week intervals, nine collections of subgingival sulcus material from five sites of the four right incisor teeth of each animal were performed and subjected to microbiological evaluation using polymerase chain reaction with primers of 25 microorganisms considered potentially pathogenic. After 1440 periodontal clinical evaluations of incisor teeth of the 10 calves, a total of 395 episodes of gingivitis were recorded, of which 267 occurred in the Control Group and 128 in the Virginiamycin Group. Similarly, 89 episodes of necrotizing gingivitis were recorded; 58 in the Control Group and 31 in the Virginiamycin Group. Comparison of between-group means found significant differences for teeth with gingivitis and necrotizing gingivitis (t test; p<0.05). The total number of teeth with gingivitis (p<0.01) and necrotizing gingivitis (p<0.01) in Control Group was significantly higher than that of gingivitis (p<0.01) and necrotizing gingivitis (p<0.05) in the Virginiamycin Group. There was a positive correlation between total occurrence of gingivitis and necrotizing gingivitis in the Virginiamycin Group by Pearson’s test. Virginiamycin had a protective effect on treated animals compared with the Control Group (OR = 0.36: CI (95%) = 0.27-0.43). In the Control Group, Actinomyces israelli (4.74%), domain Archaea (1.58%), Eikenella corrodens (1.05%), Fusobacterium nucleatum (27.37%), class Mollicutes (5.26%); Porphyromonas endodontalis(5.26%); Porphyromonas gulae(0.53%), Prevotella buccae (6.32%), Prevotella loescheii (3.68%), Prevotella nigrescens (8.42%), Prevotella oralis (1.58%), Tannerella forsythia (0.53%), and Treponema denticola (4.21%) were detected at healthy sites, and gingivitis or necrotizing gingivitis samples. In the Virginiamycin Group, A. israelli (3.41%), domain Archaea (0.98%), F. nucleatum (9.27%), class Mollicutes(4.39%), P. endodontalis (4.39%), P. gulae (0.49%), P. buccae (8.29%), P. loescheii (6.83%), P. nigrescens (15.61%), P. oralis (1.46%), Selenomonas sputigena (0.49%), T. forsythia (0.49%), and T. denticola (2.44%) were detected. In conclusion, virginiamycin administered at a dosage of 340mg/animal/day significantly reduced the occurrence of gingivitis and necrotizing gingivitis in cattle maintained on reformed pastures, and was revealed to have action against periodontal bacterial microbiota considered to be potentially pathogenic.

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“…At the time, it was considered the most important disease in cattle breeding, and limited livestock development in the Southeast and Center-West regions (Döbereiner et al 1974(Döbereiner et al , 2000. Outbreaks with high prevalence and severity also occurred in pastures replanted in previously endemic areas, which led to serious economic losses to the livestock sector (Dutra et al 1993(Dutra et al , 1999. In conventional microbiological analysis of lesions in bovine periodontitis using traditional methods of anaerobic culture, characterization and identification, it is possible to observe that approximately 80% of the microbiota of calves associated with periodontitis was composed of black pigmented colonizing bacteria when cultured in blood agar supplemented with hemin and vitamin K (Botteon et al 1993, Dutra et al 1999.…”

Section: Introductionmentioning

confidence: 99%

Subgingival bacterial microbiota associated with ovine periodontitis

et al. 2019

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Periodontitis is an inflammatory response in a susceptible host caused by complex microbiota, predominantly composed of Gram-negative anaerobic bacteria. Aiming to characterize the subgingival bacterial microbiota associated with ovine periodontitis, polymerase chain reaction (PCR) was performed in subgingival periodontal pocket samples of 14 sheep with severe periodontitis and in subgingival sulcus biofilm of 14 periodontally healthy sheep in search mainly of Gram-negative and Gram-positive microorganisms considered important periodontopathogens. The most prevalent bacteria in the sheep with periodontal lesions were Tannerella forsythia (78.6%), Treponema denticola (78.6%), Fusobacterium nucleatum (64.3%), and Porphyromonas gingivalis (50%), whereas in the healthy sheep, F. nucleatum (42.8%) was the most often detected bacterium. Statistically significant differences were observed for Campylobacter rectus, Enterococcus faecium, Prevotella nigrescens, T. forsythia, and T. denticola (p<0.05) in the sheep with periodontitis in the comparison between groups. Aggregatibacter actinomycetemcomitans, Enterococcus faecalis, and Porphyromonas gulae were not detected in any of the samples analyzed. In conclusion, C. rectus, E. faecium, P. nigrescens, T. forsythia, and T. denticola were associated with severe lesions caused by ovine periodontitis, and F. nucleatum was the most prevalent microorganism in the subgengival sulcus biofilm of healthy sheep.

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Modificação da microbiota associada às lesões peridentárias da "cara inchada" em bezerros transferidos para área indene

Cited by 17 publications

References 3 publications

Presence of Porphyromonas and Prevotella species in the oral microflora of cattle with periodontitis

Presence of Porphyromonas and Prevotella species in the oral microflora of cattle with periodontitis

Efficacy of virginiamycin for the control of periodontal disease in calves

Subgingival bacterial microbiota associated with ovine periodontitis

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