A expressão da proteína p16 e herpes simples vírus tipo 2 em lesões pré-neoplásicas e neoplásicas do colo do útero

Salcedo, Mila Pontremoli; Silveira, Gustavo Py Gomes da; Zettler, Cláudio Galeano

doi:10.1590/s0100-72032008000200003

Cited by 3 publications

(3 citation statements)

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“…Many recent studies have found the importance of p16 and p53 in cervical neoplasia 9,[12][13][14][17][18][19][20][21][22][23][24][25][26][27][28][29][30][31][32][33][34] . The vast majority rated the immunomarker positivity percentage for the presence of cervical intraepithelial neoplasia and correlated with the lesion severity 9,12,13,17,18,[21][22][23][24][25][26][27][28][29] . However, few studies related immune reaction with disease recurrence and/or prognosis 13.14-31 .…”

Section: Discussion Discussion Discussion Discussion Discussionmentioning

confidence: 99%

The role of P16ink4a and P53 immunostaining in predicting recurrence of HG-CIN after conization treatment

Fonseca

Tomasich

Jung

et al. 2016

Rev. Col. Bras. Cir.

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Section: Discussion Discussion Discussion Discussion Discussionmentioning

confidence: 99%

The role of P16ink4a and P53 immunostaining in predicting recurrence of HG-CIN after conization treatment

Fonseca

Tomasich

Jung

et al. 2016

Rev. Col. Bras. Cir.

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“…For instance, one alternative approach is based on immunocytochemical detection of cellular proteins, such as p53, p16 or HPV viral antigens [12-15 ]. Reports using these markers show that although the marker may be expressed in all grades of cervical neoplasia, in some cases, a particular grade may exhibit no demonstrable expression.…”

Section: Discussionmentioning

confidence: 99%

Novel dual-function CellDetect® staining technology: wedding morphology and tinctorial discrimination to detect cervical neoplasia

Idelevich¹,

Elkeles²,

Okon³

et al. 2010

Diagn Pathol

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BackgroundA persistent goal of oncologic histochemistry is to microscopically identify neoplasia tinctorially. Consequently, the newly developed CellDetect® staining technology, that appears to exhibit this property, warrants clinical evaluation. The objective of this study was to compare the diagnostic results using CellDetect® to the outcomes of standard microscopic examination based on hematoxylin and eosin (H&E) staining for the recognition of different squamous epithelial phenotypes of the uterine cervix.MethodsPairs of adjacent sections were made from 60 cervical biopsy cases that were diagnosed originally as either normal or neoplastic (CIN, SCC). One section of the pair was stained for H&E; the second section, with CellDetect®. Based on the examination of these pairs by two experienced pathologists, we investigated the following issues:(1) diagnostic agreement between the pathologists on each pair; (2) agreement between H&E and CellDetect® for each pair (3) tinctorial characteristics in micro-regions (n = 130) evaluated as either normal, reactive or neoplastic.ResultsQualitatively, CellDetect®-stained preparations displayed cyto-morphological detail comparable to H&E images. Tinctorially, non-neoplastic cells appeared green/blue when stained withCellDetect®, contrasting with cytologically neoplastic foci, where cells of every grade were red/magenta in color. Due to these tinctorial characteristics, even small foci of neoplasia could be readily distinguished that were inconspicuous on H&E at low magnification. In some instances, this prompted re-examination of the H&E and revision of the diagnosis. Quantitatively, we found that despite diagnostic variation between pathologists, in about 3% of the cases, each pathologist made the same diagnosis regardless of whether CellDetect® or H&E was used, i.e. there was 100% self-agreement for each pathologist between stains. Particularly noteworthy was the finding of a 0% false negative rate, coupled with a 10-15% false positive rate. Regarding specificity, the performance in reactive squamous processes was similar to that observed for morphologically normal squamous epithelium.ConclusionsIn this first order assessment of clinical applicability, CellDetect® staining technology was at least comparable to results using H&E, and perhaps surperior. CellDetect® provided a uniquely useful tinctorial clue for the detection of neoplasia, which exhibited an impressive 0% false negative rate. A more extensive, blinded study is needed to confirm these promising findings.

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“…), long term use of oral contraceptives, intrauterine device use, multiple full-term pregnancies, young age at full-term pregnancy and poverty. [2][3][4] Human Herpesviruses (HHVs) can induce cell transformation by production of oncogenic proteins or by interacting with cell replication, which are important steps for cancer development 5 For many years, CMV was one of the HHVs that was thought to have a role in the development of cervical lesions. 6 CMV infects between 50 to 85% of the worldwide population by early adulthood.…”

Section: Introductionmentioning

confidence: 99%

Cytomegalovirus Infection and Cervical Cancer: from Past Doubts to Present Questions

Marinho‐Dias

Sousa

2013

Acta Med Port

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Background: Since early 60’s that Cytomegalovirus was studied for its possible role in cervical cancer development. Despite several decades of studies and the description of CMV DNA in cervical samples, it is still doubtful what is the prevalence of Cytomegalovirus in cervix and if CMV can act as a co-factor in cervical carcinogenesis. Methods: In this Systematic Review we intend to summarize the frequency of Cytomegalovirus in cervical samples by revising all published studies between 1980 and 2011 regarding the detection of Cytomegalovirus in cervical samples and the development of lesions/ invasive cervical cancer. Crude and adjusted frequencies of Cytomegalovirus infection were calculated according to country and world region. Results: This study revealed that the worldwide crude frequency of Cytomegalovirus infection in the cervix was 18.9% in all cervical samples and 36.5% in HPV positive women. Cytomegalovirus infection was present in all different types of lesions: 17.4% in normal/ cervicitis, 28.0% in LSIL, 19.7% in HSIL and 44.4% in CIS/ICC. The overall rate of Cytomegalovirus infection varied from 1.58% to 61.0% with an increased incidence in less developed countries. Conclusion: In this study we described a high frequency of positive Cytomegalovirus cases in all types of cervical samples, with increased incidence in both HPV-infected women and CIS/ICC cases. Hence, despite results showed that Cytomegalovirus shedding in cervical samples is frequent more studies should be performed to clarify if Cytomegalovirus infection is an opportunistic infection in HPV-infected cases, or if it contributes for cervical immunosuppression that will favor HPV-associated carcinogenesis.

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A expressão da proteína p16 e herpes simples vírus tipo 2 em lesões pré-neoplásicas e neoplásicas do colo do útero

Abstract: a progressive increase of reactivity ratios of the studied immunohistochemical markers as a function of lesion severity was observed.

Cited by 3 publications

References 0 publications

The role of P16ink4a and P53 immunostaining in predicting recurrence of HG-CIN after conization treatment

The role of P16ink4a and P53 immunostaining in predicting recurrence of HG-CIN after conization treatment

Novel dual-function CellDetect® staining technology: wedding morphology and tinctorial discrimination to detect cervical neoplasia

Cytomegalovirus Infection and Cervical Cancer: from Past Doubts to Present Questions

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