Criopreservação de sêmen humano - comparação entre métodos de congelação e tipos de envase

ObjectiveThis study aimed to test the effects on sperm viability of transporting cryopreserved semen samples on dry ice.MethodsTwenty normozoospermic semen samples were cryopreserved and divided into five groups. The samples in Group 1 were immersed in liquid nitrogen throughout the experiment in cryogenic storage tanks; the cryopreserved straws in Group 2 were placed in a Styrofoam box containing dry ice and kept under these conditions for 48 hours; the samples in Group 3 were kept for 48 hours on dry ice under the same conditions as the Group 2 samples, and were then moved to a storage tank filled with liquid nitrogen; Group 4 samples were also kept for 48 hours in dry ice storage, and the Styrofoam box containing the samples was shipped by plane to assess the effects of shipping; the samples in Group 5 were shipped together with the Group 4 samples and were placed in a storage tank with liquid nitrogen after spending 48 hours stored on dry ice. After thawing, sperm parameters were analyzed for viability, vitality, and motility; spermatozoa were also tested for mitochondrial activity.ResultsSignificant decreases in motility recovery rates (P=0.01) and vitality (P=0.001) were observed in all groups when compared to the control group. Mitochondrial activity was significantly decreased only in Group 5 (P=0.04), as evidenced by greater numbers of sperm cells not stained by reagent 3,3'-diaminobenzidine.ConclusionsTransportation did not affect the quality of cryopreserved semen samples, but dry ice as a means to preserve the samples during transportation had detrimental effects upon the sperm parameters assessed in this study.

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“…Cryopreservation of human semen for use in assisted reproduction has become a globally recognized procedure (Cavalcante et al ., 2006). …”

Section: Introductionmentioning

confidence: 99%

“…Motility indicates the functional competence of sperm cells and vitality is a way to identify living and dead sperm (Cavalcante et al , 2006); the former is one of the most severely affected variables (Watson, 1995). Verza Jr et al .…”

Section: Introductionmentioning

confidence: 99%

The effects of storing and transporting cryopreserved semen samples on dry ice

Til

Amaral

Salvador

et al. 2016

JBRA Assisted Reproduction

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“…However, the efficacy of this substance may be subject to alteration according to the morphological and functional cell characteristics or tissue to be preserved [37]; concentration and type of cryoprotectant, as well as the time of exposure prior to the cryopreservation method. Currently, the that may be subordinated to radio or chemotherapy, or surgeries that may affect fertility potential, such as cryopreservation of semen for men who are about to be submitted to a vasectomy, with the aim of preserving the future fertility [69]. A study performed by Schuffnera and collaborators in 2004 [5] verified two cases of successful gestation after the semen was cryopreserved, prior to the oncological treatment.…”

Section: Cryoprotectantsmentioning

confidence: 99%

Oncofertility: An Area of Reproductive Medicine Capable to Protect the Fertility of Cancer Patients

da¹,

Perez²,

Torres³

et al. 2018

Int J Womens Health Wellness

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Cancer, also called malignant neoplasm, is a disorder which can be manifested in almost anywhere in the body as a result of errors in the cell cycle control machinery, resulting in the formation of tumors. Although the pursuit of a cure is still a top priority for cancer research, the concern about the side effects that the treatment of the disease can produce is an important factor that must be taken into account. One undesirable consequence is infertility, since patients are subordinate to the cytotoxic dynamics of radio and chemotherapy. In this sense, assisted reproduction provides an alternative to protect the fertility of the bearer, through the use of reproductive biotechniques associated with gametes, tissues and embryo freezing, either by vitrification or conventional slow freezing. Cryopreservation is a reproductive biotechnique in which extremely low temperatures are used in order to decrease cellular metabolism. This technique has gained interest in the field of oncofertility, offering hope for men and women, cancer patients, granting them the possibility of being able to procreate biological children once their treatment for cancer is concluded. Although the cryopreservation technique allows the preservation of viability to diverse tissues and cells used during in-vitro fertilization (IVF) and artificial insemination (AI), among other techniques, the low temperatures from cryopreservation can lead to irreversible cellular damage. As a result, cryoprotectants appear to contribute to the reduction of the rate of deleterious effects caused by intracellular ice formation, increasing the survival rate of viable gametes. Therefore, in the present article a bibliographic survey was carried out from 1983 to 2018, through databases such as Scielo and Pubmed. Eighty-two articles were used as reference, having a requirement for inclusion of scientific articles published in Portuguese, English and Spanish, with the purpose of describing the state of research which attempts to preserve the fertility of cancer patients.

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Criopreservação de sêmen humano - comparação entre métodos de congelação e tipos de envase

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The effects of storing and transporting cryopreserved semen samples on dry ice

The effects of storing and transporting cryopreserved semen samples on dry ice

Oncofertility: An Area of Reproductive Medicine Capable to Protect the Fertility of Cancer Patients

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