Histochemical aspects of reserves mobilization of Caesalpinia peltophoroides (Leguminosae) seeds during germination and seedlings early growth

Abstract

-The objective of this study was to investigate reserve mobilization in Caesalpinia peltophoroides seeds during germination and initial seedling growth. The variation in these compounds was analyzed from the pre-germination period (0 to 5 days after sowing -DAS) to the total cotyledon senescence and abscission at 35 DAS. For this histochemical tests were made on cotyledons fixed in FAA50 or included in glycol-metacrylate. To follow the mobilization of the main reserve compounds, sudan III was used to detect total lipids, xylidine Ponceau to detect total proteins, lugol to detect starch and polarized light to visualize the crystals. The lipids, present in a great quantity in the cotyledon, gradually decreased in the period studied. A greater quantity of starch was observed on the 10th DAS than in the previous periods and it was totally consumed by 30 DAS. The distribution pattern and the morphology of the protein material were very modified by 10 DAS, a period during which it was intensely consumed, remaining only parietally fragments distributed, that practically disappeared at 25 DAS. The calcium oxalate druses were not consumed during the period studied, there was only crystal agglutination.Keywords: Reserve materials, carbohydrates, lipids, proteins and calcium oxalate. ESTUDO HISTOQUÍMICO DA MOBILIZAÇÃO DE RESERVAS DE SEMENTES DE Caesalpinia peltophoroides (LEGUMINOSAE) DURANTE A GERMINAÇÃO E CRESCIMENTO INICIAL RESUMO -Este trabalho teve como objetivo o estudo da mobilização de reservas de sementes de Caesalpinia peltophoroides Benth. durante a germinação e crescimento inicial das plântulas. As variações nesses compostos foram analisadas desde o período pré-germinativo (0 a 5 dias após a semeadura -DAS) até a total senescência e abscisão dos cotilédones, aos 35 DAS. Para isso, foram realizados testes histoquímicos em cotilédones fixados em FAA50 ou incluídos em glicol-metacrilato. Para acompanhamento da mobilização dos principais

Histochemical analysis of seed reserve mobilization in Passiflora edulis Sims fo. flavicarpa O. Deg. (yellow passion fruit) during germination

“…Electrophoretic analysis of protein extractions (unpublished data) showed that as germination takes place, there is a reduction in high molecular weight bands whereas low molecular weight bands become more evident. A similar event was observed by Corte et al (2008), during protein mobilization in C. peltophoroides seeds. However, the authors reported that there was an increase in the size of the protein body preceding its content degradation, which remains confined to a small space, whilst the same was not observed in our results.…”

“…Other species seeds maintained stable lipid levels during the early period of germination, only consuming them at the late stages, as observed in the Ricinus communis L. seeds (Muto and Beevers, 1974), where the lipid content remains at the same levels until the 3 rd day from sowing, disappearing almost completely on the 7 th day, with root-shoot axis length of about 15 cm, and in seeds of Caesalpinia peltophoroides Benth. (Corte et al, 2006(Corte et al, , 2008 with lipid levels diminishing during the first ten days from sowing, with complete consumption until the 20 th day. Probably, the lipid present in the endosperm is used as a source material for the synthesis of carbohydrates as a positive response to PAS in the cotyledons appears just after the lipid levels start to diminish, around t6.…”

Alternating temperature and accelerated aging in mobilization of reserves during germination of Carica papaya L. seeds

“…As of quantification and evaluation of the mobilization of lipids during germination of Apuleia leiocarpa, it was seen that the energetic and structural needs of the germination are supplied by lipids (Pontes et al, 2002). Corte et al (2008) suggest the direct involvement of the lipid reserves as energy supply for germination and growth of Caesalpinia peltophoroides seedlings. In papaya seeds, the use of substances like KNO 3 , effective in increasing germination and in overcoming dormancy, promote greater mobilization of lipids (Zanotti et al, 2014).…”

Development of a UV/Vis spectrophotometric method for analysis of total polyphenols from Caesalpinia peltophoroides Benth

“…3 It is a domesticated tree, which in Brazil is found mainly in the Atlantic Forest and the Pantanal. C. peltophoroides is used as an ornamental tree in cities and has a potential use for timber, 4 and in Bolivia the bark is popularly used to treat dysentery. 5 Previous studies using a 70% ethanol extract from the bark of C. peltophoroides showed in vitro antimalarial activity against chloroquine-resistant Plasmodium falciparum, with an IC 50 of 8.3 µg/mL.…”