Constatação de Bipolaris bicolor em plantas de açaizeiro no Brasil

A fungal Bipolaris bicolor strain as a potential bioherbicide for goosegrass (Eleusine indica) control

“…This species occurs generally on plants in warm temperate and tropical regions 26,27 . There are reports of B. bicolor being isolated from plant leaf spot and root rot affected St. Augustine grass ( Stenotaphrum secundatum ), 28 signalgrass ( Brachiaria decumbens ), 29 hemp palm ( Trachycarpus fortunei ) 30 and the rubber tree ( Ficus elastica ) 31 . In 2017, although the strain CPC28811 of B. bicolor was first reported from new host goosegrass in Thailand, no descriptions about its morphological characteristics and disease symptoms were given 27 .…”

“…The reaction system with a 25 μL volume contained 12.5 μL 2 × Hieff PCR Master Mix with Dye (Yeasen Biotech Co., Ltd, Shanghai, China), 1 μL of each 10 μM primer, 1 μL of template DNA and 9.5 μL of sterilized distilled water. The PCR reaction programs were as follows: an initial denaturing step at 94 °C for 5 min, followed by 30…”

“…After 20-μL conidial suspensions of 5 × 10 5 were dripped onto a glass slide with a grooved surface and dried in air on a superclean bench, 20 μL of PDB liquid media (potato 200 g L −1 , sucrose 20 g L −1 ) with different pH (1-12) were added onto the grooved glass slides and incubated for 6 h at 28 °C and 100% relative humidity in darkness, or the dried conidia on grooved glass slides were directly placed in different relative humidity conditions (69%, 75%, 85%, 90%, 94%, 98% and 100%) and incubated for 12 h at 28 °C in darkness. To evaluate the effect of temperature and light on conidial germination, 20 μL of conidial suspensions of 5 × 10 5 were dripped onto the grooved glass slides and incubated for 6 h at various temperatures (15,20,25,30,35,40,45,49, 50 and 51 °C) under dark or at 28 °C under various illuminations (dark, fluorescence light, UV light and black light). More than 100 conidia were counted for each treatment.…”