Caracterização cultural, morfológica e patogênica de Lasiodiplodia theobromae associado a frutíferas tropicais

Lima, Joílson Silva; Moreira, Renato Cesar; Cardoso, J. E.; Martins, M. V. V.; Viana, F. M. P.

doi:10.1590/s0100-54052013000200001

Cited by 20 publications

(17 citation statements)

References 10 publications

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“…theobromae , although it causes disease in more than 500 plant species [42–44] and is endemic to tropical and subtropical regions. It can also colonize plant tissues without any visible symptoms of infection and live as an endophyte or saprophyte [43,45–48]. The change from a non-pathogenic lifestyle to a disease-causing pathogen may be associated with host stress [45,48,49].…”

Section: Discussionmentioning

confidence: 99%

Seedborne Pathogenic Fungi in Common Bean (Phaseolus vulgaris cv. INTA Rojo) in Nicaragua

Marcenaro

Valkonen

2016

PLoS ONE

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Common bean (Phaseolus vulgaris L.) is an important legume with high nutritional value. In Nicaragua, certified healthy seeds of local bean varieties are not available, and seedborne fungi have gained little attention. Here, were surveyed seedborne pathogenic fungi in an important local bean cultivar, ‘INTA Rojo’. Beans grown in the four main production areas in Nicaragua (Boaco, Carazo, Estelí, Matagalpa) for future use as seed stock were sampled from four seed storehouses and six seed lots. A total of 133 fungal strains were isolated from surface-sterilized beans and inoculated to healthy lima beans (Phaseolus lunatus) under controlled conditions. Eighty-seven isolates caused symptoms of varying severity in the seedlings, including discoloration, necrotic lesions, cankers, rot, and lethal necrosis. Pathogenic isolates were divided into eight phenotypically distinguishable groups based on morphology and growth characteristics on artificial growth medium, and further identified by analysis of the internal transcribed spacer sequences (ITS1 and ITS2) of the ribosomal RNA genes. The pathogenic isolates belonged to eight genera. Fusarium spp. (F. chlamydosporum, F. equiseti, F. incarnatum), Lasiodiplodia theobromae, Macrophomina phaseolina, and Penicillium citrinum were the most damaging and common fungi found in the seed lots. Furthermore, Corynespora cassiicola, Colletotrichum capsisi, Colletotrichum gloeosporioides, Aspergillus flavus, and Diaporthe sp. (Phomopsis) were seedborne in cultivar ‘INTA Rojo’ and found to be pathogenic to bean seedlings. This study reveals, for the first time, many seedborne pathogenic fungi in beans in Nicaragua; furthermore, prior to this study, little information was available concerning F. equiseti, F. incarnatum, L. theobromae, C. cassiicola, and Diaporthe spp. as seedborne pathogens of common bean. Our results lay the basis for developing diagnostic tools for seed health inspection and for further study of the epidemiology, ecology, and control of the pathogenic fungi of common beans in the field.

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Section: Discussionmentioning

confidence: 99%

Seedborne Pathogenic Fungi in Common Bean (Phaseolus vulgaris cv. INTA Rojo) in Nicaragua

Marcenaro

Valkonen

2016

PLoS ONE

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“…Esta espécie também foi a mais agressiva e foi isolada como endofítica de 19 hospedeiros nativos e introduzidos na Caatinga do estado do Ceará. Vários estudos mostram que L. theobromae não apresenta especificidade por hospedeiro (17,18,19) e, o comportamento dessa espécie como fungo endofítico e patógeno latente já foi relatado em uma diversidade de plantas hospedeiras (24,30). Este estudo reforça a elevada frequência de L. theobromae como patógeno latente e/ou endofítico em plantas do bioma Caatinga, situando-o como uma ameaça as plantas de interesse agrícola e florestal nessa área.…”

Section: Resultsunclassified

“…& Maubl. foi relatado causando doenças na maioria das espécies de frutíferas estudadas nesse estado (10,11,17,18,19). No entanto, estudos realizados em outros locais mostram que espécies de Botryosphaeriacae associadas a estas mesmas frutíferas formam um grupo diverso (7,22,23).…”

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Patogenicidade de espécies de Botryosphaeriaceae endofíticas de plantas da Caatinga do estado do Ceará em manga e umbu-cajá

et al. 2016

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Espécies de Botryosphaeriaceae são importantes patógenos em diversas plantas lenhosas e não lenhosas, causando diferentes tipos de sintomas. Espécies desta família são usualmente consideradas patógenos fracos, causando doenças apenas quando estas plantas se encontram sob algum tipo de estresse como seca, temperaturas baixas ou elevadas, deficiência nutricional e danos causados por outros patógenos ou pragas. No entanto, pouco se conhece sobre a ocorrência e diversidade de espécies de Botryosphaeriaceae em plantas nativas do bioma Caatinga, localizado no Semiárido, e sobre a presença de fungos endofíticos nessas espécies, e muito menos sobre a sua importância como patógenos. O objetivo do presente estudo foi avaliar a patogenicidade de 74 isolados representando Espécies de Botryosphaeriaceae (Dothideomycetes) têm distribuição mundial, sendo encontradas em todas as regiões geográficas, incluindo áreas de clima tropical, temperado e até em ambiente marinho, com exceção das regiões polares (2,34,35,38). Já foram relatadas infectando plantas do grupo das monocotiledôneas, dicotiledôneas e gimnospermas, e até liquens (1). Seus hospedeiros incluem uma diversidade de plantas de importância agronômica, florestal e ornamental (1, 8). Entre as doenças causadas por espécies de Botryosphaeriaceae estão podridão de frutos, manchas foliares, tombamento e podridão de colo em mudas, cancros, secas em brotos e mudas, gomose, seca descendente, vassoura-de-bruxa e morte de árvores (41).Espécies de Botryosphaeriaceae são frequentemente descritas como oportunistas ou patógenos latentes, os quais apresentam longo período de latência antes que algum fator como, por exemplo, estresse hídrico faça com que eles se tornem patogênicos, causando, frequentemente, cancros em seus hospedeiros (6,28,42,45). Essas espécies são bem conhecidas como endofíticas em uma variedade de plantas arbóreas. Entre as principais famílias botânicas já estudadas incluem-se Moraceae, Myrtaceae, Proteacea, Leguminosae, Pinaceae, Vitaceae e Anacardiaceae (6, 8, 9,15,16,24,27,29,42,43,44).No Ceará, o bioma Caatinga cobre uma área de 126.926 km 2 , ABSTRACTSpecies of the Botryosphaeriaceae are important pathogens in several woody and non-woody plants, causing different types of symptoms. Species of this family are usually regarded as weak pathogens, only causing diseases when plants are under some type of stress such as drought, hot or cold weather, poor nutrition and damage caused by other pathogens or pests. However, little is known about the occurrence and diversity of Botryosphaeriaceae species in native plants of the biome Caatinga, located in the Brazilian Semi-Arid Region, and about the presence of endophytic fungi in these species; even less is known about their importance as pathogens. The aim of the present study was to assess the pathogenicity of 74 isolates representing the species Botyosphaeria mamane, Pseudofusicoccum adansoniae, P. stromaticum, the complex Neofusicoccum parvum/ribis, Lasiodiplodia gonubiensis and L.theobromae, identified for the first ...

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“…Griffon & Maubl. is a pathogen with a worldwide distribution in tropical and subtropical regions, with low host specificity, causing many diseases in several species of plants (Lima et al, 2013;Muniz et al, 2014). According to the Systematic Mycology and Microbiology Laboratory Fungus-Host Distributions Database, there are around 638 literature records distributed in 71 locations around the world and 360 hosts (Farr and Rossman, 2015) associated with this pathogen.…”

Section: Introductionmentioning

confidence: 99%

Phylogeography and population structure analysis reveals diversity by mutations in Lasiodiplodia theobromae with distinct sources of selection

Santos¹,

Carvalho²,

Aguiar³

et al. 2017

Genet. Mol. Res.

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ABSTRACT. Lasiodiplodia theobromae is a plant pathogen with a worldwide distribution, with low host specificity, causing stem cankers, dieback diseases, and fruit rot in several species of plants. In coconut, this pathogen is reported as the etiological agent of "coconut leaf blight" (CLB) disease, causing several losses in fruit production. The CLB is an important disease for this crop in Brazil. In our study, we used a phylogeographic approach through the molecular characterization of the translation elongation factor 1-α (TEF1-α) to elucidate the pathogen distribution in Brazil and other countries, besides, search information about diversity sources of this pathogen in coconut palm tree at Brazilian northern, northeast, and southeast. We found that L. theobromae diversity is within populations (locations), and populations that are located closest to the center of the tropical zone have more variability as Central Africa, Brazilian Southeast, and Northeast. The widespread distribution could be in part related with long-distance dispersal via global trade of plants and plant products. The entrance route of L. theobromae in Brazil probably occurred from Africa route and not occurred once. In Brazil, the diversity of this pathogen in coconut tree could be linked to two agents of selection: high host diversity (in Northeast) and distinct management measures adopted in Southeast. These different sources of selection, mainly the mutations, could be one of the reasons that we found distinct reactions to "coconut leaf blight" chemical control in these regions.

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Caracterização cultural, morfológica e patogênica de Lasiodiplodia theobromae associado a frutíferas tropicais

Cited by 20 publications

References 10 publications

Seedborne Pathogenic Fungi in Common Bean (Phaseolus vulgaris cv. INTA Rojo) in Nicaragua

Seedborne Pathogenic Fungi in Common Bean (Phaseolus vulgaris cv. INTA Rojo) in Nicaragua

Patogenicidade de espécies de Botryosphaeriaceae endofíticas de plantas da Caatinga do estado do Ceará em manga e umbu-cajá

Phylogeography and population structure analysis reveals diversity by mutations in Lasiodiplodia theobromae with distinct sources of selection

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