Detecção de allexivírus em primórdios foliares de alho via RT-PCR

Abstract

Cloves crop is a routinely practice often used in procedures for detecting allexiviruses in garlic bulbs to obtainment foliar tissue to be analyzed by serological and/or molecular tests. The availability of the plants under greenhouse implies is expenses with the maintenance and requires abaut 30 days. In areas free of these viruses, there is also t h e r i s k of t h e i r i n t r od u c t i on a n d d i s s em i n a t i on . T h i s s t u d y presen ts an adju stm ent of protoc ol a imi ng a fa st det ecti on of allexiviruses, using primordial leaves. Cloves of consumptiong a rl i c , f r o m R i o Gr a n d e d o S u l , B r a z i l , a n d i m p o rt e d fr o m Argentina were dissected for obtaining primordial leaves and total Nascimento, R.J.; Pio-Ribeiro, G.; Santos, R.C.; Melo Filho, P.A. Detection of allexivírus on primordial garlic leaves using RT-PCR. Summa Phytopathologica, v.34, n.3, p.267-269, 2008

In Vitro Elimination of Dahlia Mosaic Virus by Using Meristem Culture, Electrotherapy and Chemotherapy

“…Increasing in acyclovir concentrations typically increases the effectiveness of virus elimination, but at higher concentrations especially at 50mg/l slowed plant growth and phytotoxicity was evident. The required concentrations of many antivirals, that is necessary to inhibit virus multiplication, are very close to that point induce toxicity for host plants (26) thus at 40mg/l and 30mg/l survival rates decreased slowly but the number of the produced healthy plants was higher for both acyclovir and salicylic acid. In general, antivirals at effective low concentrations hinder virus replication.…”

Identification and sequence analysis of five allexiviruses species infecting garlic crops in Brazil

“…These include species belonging to the genera Potyvirus and Carlavirus (both aphid-borne viruses) and Allexivirus (mite-borne viruses). In Brazil, the allexivirus species reported infecting garlic are Garlic virus A (GarV-A), Garlic virus B (GarV-B), Garlic virus C (GarV-C), Garlic virus D (GarV-D), Garlic-mite filamentous virus (GarMbFV) (Nascimento et al, 2008) and more recently Garlic virus X (GarV-X) (Oliveira et al, 2014). These viruses have a single component of a single-stranded, positive sense, RNA with a 3'-poly A tail, but differ in genome organization.…”