Efficiency of phylloplane bacteria in controlling aerial tomato diseases under field conditions

Halfeld-Vieira, Bernardo A.; Romeiro, R. S.; Mounteer, Ann Honor; Mizubuti, Eduardo S. G.

doi:10.1590/s0100-54052008000100019

Cited by 6 publications

(4 citation statements)

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“…viticola (Tilcher et al 1994(Tilcher et al , 2002, while bacteria, mainly belonging to the genera Bacillus and Pseudomonas (Ps. ), have so far given the most interesting and promising results in controlling P. infestans on tomato plants (Yan et al 2002;Kloepper et al 2004;Tran et al 2007;Halfeld-Vieira et al 2008;Lamsal et al 2013). Some of these strains indirectly reduce the attacks of P. infestans by triggering induced systemic resistance in tomato plants (Yan et al 2002;Tran et al 2007).…”

Section: Introductionmentioning

confidence: 99%

Lysobacter capsici AZ78 produces cyclo(l -Pro-l -Tyr), a 2,5-diketopiperazine with toxic activity against sporangia of Phytophthora infestans and Plasmopara viticola

et al. 2014

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The application of L. capsici AZ78 cells or its own culture filtrates effectively controls both P. infestans and Pl. viticola. Cyclo(L-Pro-L-Tyr) produced by L. capsici AZ78 is toxic against sporangia of both these oomycetes. These data enforce the potential in the use of Lysobacter members for the control of plant pathogenic oomycetes and provide the basis for the development of new low-impact fungicides based on cyclo(L-Pro-L-Tyr).

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Section: Introductionmentioning

confidence: 99%

Lysobacter capsici AZ78 produces cyclo(l -Pro-l -Tyr), a 2,5-diketopiperazine with toxic activity against sporangia of Phytophthora infestans and Plasmopara viticola

et al. 2014

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“…These results demonstrate the use potential of those biocontrol agents against two important tomato diseases. Halfeld-Vieira et al (2008) proved the efficiency of the epiphyte Bacillus cereus UFV-IEA6 against Phytophthora infestans.…”

Section: Resultsmentioning

confidence: 98%

“…However, the biocontrol of diseases affecting several crops by those microorganisms have been increasingly researched (Lindow & Brandl, 2003;Halfeld-Vieira et al, 2008). Notably, the interest in the study of those microbes is related to their capacity to occupy ecological niches on the phylloplane that could be occupied by pathogens (Monier & Lindow, 2005a), and to their broad antagonistic effect against pathogens.…”

Section: Introductionmentioning

confidence: 99%

Bacterial spot and early blight biocontrol by epiphytic bacteria in tomato plants

Filho

Silva

Alves

2010

Pesq. agropec. bras.

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-The objective of this work was to evaluate in vitro and in vivo biocontrol of bacterial spot (Xanthomonas vesicatoria) and early blight (Alternaria solani) by the epiphytic bacteria Paenibacillus macerans and Bacillus pumilus. Tomato plants were previously sprayed with epiphytic bacteria, benzalkonium chloride and PBS buffer and, after four days, they were inoculated with A. solani and X. vesicatoria. To determine the phytopathogenic bacteria population, leaflet samples were collected from each treatment every 24 hours, for seven days, and plated on semi-selective medium. The effect of epiphytic bacteria over phytopathogens was performed by the antibiosis test and antagonistic activity measured by inhibition zone diameter. The epiphytic and benzalkonium chloride drastically reduced the severity of early blight and bacterial spot in comparison to the control (PBS). In detached leaflets, the epiphytic bacteria reduced in 70% the number of phytopathogenic bacteria cells in the phylloplane. The antibiosis test showed that the epiphytic bacteria efficiently inhibit the phytopathogens growth. In all the bioassays, the epiphytic bacteria protect tomato plants against the phytopathogens.Index terms: Alternaria solani, Bacillus pumilus, Paenibacillus macerans, Xanthomonas vesicatoria, biological control, epiphytic bacteria. Biocontrole da mancha-bacteriana e da pinta-preta por bactérias epifíticas em tomateiro Resumo -O objetivo deste trabalho foi avaliar o biocontrole in vitro e in vivo dos fitopatógenos manchabacteriana (Xanthomonas vesicatoria) e pinta-preta (Alternaria solani) pelas bactérias epifíticasPaenibacillus macerans e Bacillus pumilus. Plantas de tomate foram previamente pulverizadas com as bactérias epifíticas, cloreto de benzalcônio e tampão PBS e, após quatro dias, receberam inoculação com A. solani e X. vesicatoria. A fim de determinar a população da bactéria fitopatogênica, amostras de folíolos foram coletadas de cada tratamento em intervalos de 24 horas, durante sete dias, e inoculadas em meio semisseletivo. O efeito das bactérias epifíticas sobre os fitopatógenos foi realizado pelo teste de antibiose e atividade antagônica avaliada pelo diâmetro da zona de inibição. As bactérias epifíticas e o cloreto de benzalcônico reduziram drasticamente a severidade da pinta-preta e da mancha-bacteriana, comparado com o controle (PBS). Em folíolos destacados, as bactérias epifíticas reduziram em até 70% o número de células da bactéria fitopatogênica no filoplano. As bactérias epifíticas inibem eficientemente o crescimento dos fitopatógenos em meio de cultura. Em todos os bioensaios, as bactérias epifíticas protegem as plantas de tomate contra os fitopatógenos.Termos para indexação: Alternaria solani, Bacillus pumilus, Paenibacillus macerans, Xanthomonas vesicatoria, controle biológico, bactéria epifítica.

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“…However, T. tyrosinosolvens, which belongs to Actinomycetes, has not previously been reported as an antagonistic bacterial species against plant diseases; however, a strain of species belonging to the genus Tsukamurella has been isolated from the gut of Japanese honeybee ( Apis cerana japonica ) ( Yoshiyama and Kumura, 2009 ), and from the samples of patients with cardiac pacemaker implants and chronic lung infections ( Yassin et al, 1997 ). In the case of N. capsulatum, Halfeld-Vieira et al (2008) observed that the species was capable of reducing late blight of tomato caused by Phytophthora infestans under field condition. The results of bacterial identification of the selected strains, therefore, indicate that the use of the sequential screening procedure has resulted in the selection of diverse bacterial resources for controlling pepper diseases and promoting plant growth.…”

Section: Discussionmentioning

confidence: 98%

Biocontrol of Phytophthora Blight and Anthracnose in Pepper by Sequentially Selected Antagonistic Rhizobacteria against Phytophthora capsici

Sang

Shrestha

Kim

et al. 2013

The Plant Pathology Journal

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We previously developed a sequential screening procedure to select antagonistic bacterial strains against Phytophthora capsici in pepper plants. In this study, we used a modified screening procedure to select effective biocontrol strains against P. capsici; we evaluated the effect of selected strains on Phytophthora blight and anthracnose occurrence and fruit yield in pepper plants under field and plastic house conditions from 2007 to 2009. We selected four potential biocontrol strains (Pseudomonas otitidis YJR27, P. putida YJR92, Tsukamurella tyrosinosolvens YJR102, and Novosphingobium capsulatum YJR107) among 239 bacterial strains. In the 3-year field tests, all the selected strains significantly (P < 0.05) reduced Phytophthora blight without influencing rhizosphere microbial populations; they showed similar or better levels of disease suppressions than in metalaxyl treatment in the 2007 and 2009 tests, but not in the 2008 test. In the 2-year plastic house tests, all the selected strains significantly (P < 0.05) reduced anthracnose incidence in at least one of the test years, but their biocontrol activities were variable. In addition, strains YJR27, YJR92, and YJR102, in certain harvests, increased pepper fruit numbers in field tests and red fruit weights in plastic house tests. Taken together, these results indicate that the screening procedure is rapid and reliable for the selection of potential biocontrol strains against P. capsici in pepper plants. In addition, these selected strains exhibited biocontrol activities against anthracnose, and some of the strains showed plant growth-promotion activities on pepper fruit.

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Efficiency of phylloplane bacteria in controlling aerial tomato diseases under field conditions

Cited by 6 publications

References 4 publications

Lysobacter capsici AZ78 produces cyclo(l -Pro-l -Tyr), a 2,5-diketopiperazine with toxic activity against sporangia of Phytophthora infestans and Plasmopara viticola

Lysobacter capsici AZ78 produces cyclo(l -Pro-l -Tyr), a 2,5-diketopiperazine with toxic activity against sporangia of Phytophthora infestans and Plasmopara viticola

Bacterial spot and early blight biocontrol by epiphytic bacteria in tomato plants

Biocontrol of Phytophthora Blight and Anthracnose in Pepper by Sequentially Selected Antagonistic Rhizobacteria against Phytophthora capsici

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