The Germplasm Active Bank (BAG) of banana is the base of the genetic breeding program of Embrapa Cassava and Tropical Fruits. The objective of this work was to index the accessions of the BAG for Banana streak virus (BSV). Each sample collected in the 220 accessions of BAG was used to inoculate three 'Caipira' banana plants, produced by micropropagation. The plants were inoculated using the mealybug Planococcus citri Risso as vector.