Caracterização morfológica e identificação molecular de isolados de Fusarium graminearum associados à giberela do trigo e triticale no sul do Brasil

Angelotti, Francislene; Tessmann, Dauri José; Alves, Tatiane Cristina Albuquerque; Vida, João Batista; Filho, David de Souza Jaccoud; Harakava, Ricardo

doi:10.1590/s0100-54052006000200013

Cited by 10 publications

(6 citation statements)

References 5 publications

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“…Therefore, sequencing of the TEF-1α region raised doubts regarding the identification of F 3 at the species level, supporting that this isolate belongs to the F. graminearum species complex. Angelotti et al (2006) performed morphological characterization of F. graminearum isolates associated with Fusarium head blight of wheat and triticale in the southern region of Brazil using some of the characteristics that were used in this study, including culture pigmentation on PDA medium, the dimensions of macroconidia on CLA medium, the number of macroconidial septa, and the presence of chlamydospores. The authors used these characteristics and molecular techniques as well as PCR, and they identified 20 of the isolates collected as F. graminearum.…”

Section: Resultsmentioning

confidence: 99%

Morphological and molecular characterization of Fusarium spp pathogenic to pecan tree in Brazil

Lazarotto¹,

Milanesi²,

Muniz³

et al. 2014

Genet. Mol. Res.

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ABSTRACT. The occurrence of Fusarium spp associated with pecan tree (Carya illinoinensis) diseases in Brazil has been observed in recent laboratory analyses in Rio Grande do Sul State. Thus, in this study, we i) obtained Fusarium isolates from plants with disease symptoms; ii) tested the pathogenicity of these Fusarium isolates to pecan; iii) characterized and grouped Fusarium isolates that were pathogenic to the pecan tree based on morphological characteristics; iv) identified Fusarium spp to the species complex level through TEF-1α sequencing; and v) compared the identification methods used in the study. Fifteen isolates collected from the inflorescences, roots, and Fusarium spp pathogenic to pecan tree seeds of symptomatic plants (leaf necrosis or root rot) were used for pathogenicity tests. Morphological characterization was conducted using only pathogenic isolates, for a total of 11 isolates, based on the mycelial growth rate, sporulation, colony pigmentation, and conidial length and width variables. Pathogenic isolates were grouped based on morphological characteristics, and molecular characterization was performed by sequencing TEF-1α genes. Pathogenic isolates belonging to the Fusarium chlamydosporum species complex, Fusarium graminearum species complex, Fusarium proliferatum, and Fusarium oxysporum were identified based on the TEF-1α region. Morphological characteristics were used to effectively differentiate isolates and group the isolates according to genetic similarity, particularly conidial width, which emerged as a key morphological descriptor in this study.

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Section: Resultsmentioning

confidence: 99%

Morphological and molecular characterization of Fusarium spp pathogenic to pecan tree in Brazil

Lazarotto¹,

Milanesi²,

Muniz³

et al. 2014

Genet. Mol. Res.

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“…The principal causal agents of FHB worldwide are members of the Fusarium graminearum species complex (hereafter referred to as the Fg complex), representing at least nine phylogenetically distinct species (O'Donnell et al ., 2004). Previous reports in Brazil suggested that F. graminearum sensu stricto (teleomorph Gibberella zeae ) was the principal causal agent of FHB in wheat (Angelotti et al ., 2006).…”

Section: Introductionmentioning

confidence: 99%

Trichothecene mycotoxin genotypes ofFusarium graminearum sensu strictoandFusarium meridionalein wheat from southern Brazil

et al. 2009

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A total of 82 fungal isolates was obtained from wheat kernel samples affected by fusarium head blight collected from 20 locations in southern Brazil. Polymerase chain reaction (PCR) assays were used to characterize trichothecene mycotoxin genotypes [deoxynivalenol (DON), nivalenol (NIV) and two acetylated derivatives of DON]. To identify isolates that producing DON and NIV, portions of the Tri13 gene were amplified. To identify 3-acetyl-deoxynivalenol (3-ADON) and 15-acetyl-deoxynivalenol (15-ADON) genotypes, portions of Tri3 and Tri12 were amplified. Nearly all of the isolates studied (76/82) were of the DON/15-ADON genotype. Six of the isolates were of the NIV genotype. The DON/3-ADON genotype was not observed. Portions of three genes were sequenced from representative isolates of the NIV and DON/ 15-ADON genotypes and compared with sequences from curated reference isolates of Fusarium in GenBank. blast queries for individual gene sequences and pairwise comparisons of percentage identity and percentage divergence based on 1676 bp of concatenated DNA sequence suggested that the isolates representing the DON/15-ADON genotype were Fusarium graminearum sensu stricto and the isolates representing the NIV genotype were Fusarium meridionale . This is the first detailed report of trichothecene mycotoxin genotypes of F. graminearum and F. meridionale in Brazil.

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“…All information on primer sequences and amplicon sizes for the respective trichothecene mycotoxin genotypes is summarized in Table 3. PCR assays were conducted using 400 ng of fungal DNA in a total volume of 25 µL containing 1.5 mM MgCl 2 , 2U of Platinum Taq DNA polymerase (Invitrogen, USA), and 0.2 mM of each dNTPs. Positive controls consisted of the DNA isolated from F. graminearum strains 21, 41, and 45 that were obtained from wheat and were described previously (ANGELOTTI et al, 2006;GERALDO;TESSMANN;KEMMELMEIER, 2005;BIAZIO et al, 2008). The DNA of these strains was extracted as described above.…”

Section: Trichothecenes Genotyping Of the F Graminearum Isolatementioning

confidence: 99%

Use of the polymerase chain reaction for detection of Fusarium graminearum in bulgur wheat

et al. 2012

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The detection of mycotoxigenic fungi in foodstuff is important because their presence may indicate the possible associated mycotoxin contamination. Fusarium graminearum is a wheat pathogen and a producer of micotoxins. The polymerase chain reaction (PCR) has been employed for the specific identification of F. graminearum. However, this methodology has not been commonly used for detection of F. graminearum in food. Thus, the objective of the present study was to develop a molecular methodology to detect F. graminearum in commercial samples of bulgur wheat. Two methods were tested. In the first method, a sample of this cereal was contaminated with F. graminearum mycelia. The genomic DNA was extracted from this mixture and used in a F. graminearum specific PCR reaction. The F. graminearum species was detected only in samples that were heavily contaminated. In the second method, samples of bulgur wheat were inoculated on a solid medium, and isolates having F. graminearum culture characteristics were obtained. The DNA extracted from these isolates was tested in F. graminearum specific PCR reactions. An isolate obtained had its trichothecene genotype identified by PCR. The established methodology could be used in surveys of food contamination with F. graminearum.

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Caracterização morfológica e identificação molecular de isolados de Fusarium graminearum associados à giberela do trigo e triticale no sul do Brasil

Cited by 10 publications

References 5 publications

Morphological and molecular characterization of Fusarium spp pathogenic to pecan tree in Brazil

Morphological and molecular characterization of Fusarium spp pathogenic to pecan tree in Brazil

Trichothecene mycotoxin genotypes ofFusarium graminearum sensu strictoandFusarium meridionalein wheat from southern Brazil

Use of the polymerase chain reaction for detection of Fusarium graminearum in bulgur wheat

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