Spectrophotometric determination of ofloxacin in pharmaceuticals and human urine

Abstract

Three simple and sensitive spectrophotometric methods are described for the determination of ofloxacin (OFX) in pharmaceuticals and in spiked human urine. First and second methods are based on the measurement of absorbance of OFX in 0.1 M HCl at 293 nm (method A) and 0.1 M NaOH at 287 nm, respectively. The third method is based on the measurement of 2:1 complex formed between OFX and iron(III) in H 2 SO 4 medium, the complex peaking at 420 nm (method C). The optimum conditions for all the three methods are optimized. Beer's law is obeyed over the ranges 0.63-12.5 using method A and method B, and 10-120 µg mL -1 using method C. The apparent molar absorptivity values are calculated to be 3.5 × 10 4 , 2.76 × 10 4 and 2.51 × 10 3 L mol -1 cm -1 for method A, method B and method C, respectively. The Sandell sensitivity, limit of detection (LOD) and limit quantification (LOQ) values are also reported. All the methods were validated in accordance with current ICH guidelines. The developed methods were employed with high degree of precision and accuracy for the estimation of total drug content in commercial tablet formulations of DOX. The results obtained from human spiked urine are satisfactory and recovery values are in the range 95.5-106.6%.

Reversed flow-injection method for estimation of chlorpromazine in pharmaceuticals and urine samples using charge-transfer complexation

“…Urine samples were collected from healthy volunteers and kept frozen until use after gentle thawing. The spiked urine samples were prepared according to previous published work [22] by dissolving of 6.25 mg of standard CLP in 5 mL distilled water. Subsequently a 12.5 mL aliquot of urine was transferred into 25 mL volumetric flask and diluted with distilled water to attain a concentration of 250 μg/mL of the spiked CLP.…”

FIA– Spectrophotometric methods for the determination of Naringenin in supplements and urine samples using diazotization coupling reactions

“…More diluted solutions were prepared by simple dilution. Preparation of urine samples [11]: 5 mg of pure NG transferred into 25 mL volumetric flask and then dissolved in 5 mL of ethanol. A volume of 12.5 mL of urine (supplied from healthy volunteers) was added, the volume was completed to the mark with ethanol.…”

Selective Potentiometric Sensors for the Determination of Quetiapine Fumarate in Pharmaceuticals and Spiked Human Urine

“…High-performance liquid chromatography (HPLC) [4][5][6][7][8][9][10][11][12], ultra-performance liquid chromatographic with tandem mass spectrometry (UPLC-MS) [13,14], HPLC with different detection systems such as chemiluminescence [15], electrospray ionization mass spectrometry (ESI-MS) [16][17][18][19] and tandem mass spectrometry [20][21][22][23][24], and gas chromatography [25,26] techniques have been used for the assay of QTF in body fluids. However, QTF has been determined in pharmaceuticals by titrimetric [27,28], potentiometric [29], polarographic [30], differential pulse and square wave voltammetric [31], capillary zone electrophoretic [32,33], high performance thin layer chromatographic (HPTLC) [34][35][36], HPLC [12,[37][38][39][40][41], UPLC [42] and spectrophotometric [28,32,[43][44]…”