Inhibitors of Clostridium histolyticum colagenase

Abstract

Different substances were tested as inhibitors of Clostridium histolyticum collagenase, both experimental and theoretically. The in vitro collagenase activity, alone and in the presence of inhibitors, was quantified by reaction with bovine collagen and dosage of the releasing amino acids. Collagenase-inhibitor interaction was studied theoretically by docking computational calculations. Only one among the tested substances showed inhibitor activity against the bacterial collagenase.

Inhibitors of human collagenase, MMP1

“…No stimulating or activating effects were observed in any case. On the other hand, we observed a similar activating effect of the alendronate upon the activity of the collagenase produced by the bacterium Clostridium histolyticum, one of the causative agents of gas gangrene [34]. Docking calculations defined the best geometry for ligand-enzyme interaction.…”