Métodos de preservação e crescimento de Xanthomonas campestris pv. viticola em meio de cultura variando temperatura, pH e concentração de NaCl

Peixoto, Ana Rosa; Mariano, R. L. R.; Gama, Marco Aurélio Siqueira da

doi:10.1590/s0100-41582005000600014

Cited by 6 publications

(6 citation statements)

References 2 publications

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“…The phenotypic characterization of the 31 pigmented strains of Xanthomonas (Table 1), which were causal agents of Xanthotnonas spot, was conducted as previously described by Schaad et al (24). Additionally, the growth of three pigmented strains of Xan-Iliomonas (TAQ18, TAQ13, and CCP76) was analyzed at different tetnperatures, pH levels, and salt concentrations as described by Nascimento et al (13). anacardii strains (IBSBFI508 and IBSBFI971), causal agents of angular leaf spot in cashew, one pigmented (1BSBF873) and two nonpigmented strains (1BSBF657 and IBSBF123O) isolated from mango bacterial black spots and identified as X. campestris pv.…”

Section: Methodsmentioning

confidence: 99%

Polyphasic Characterization of Pigmented Strains of Xanthomonas Pathogenic to Cashew Trees

et al. 2011

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Gama, M. A. S.. Mariano, R. L. R., Viana, F. M. P, Ferreira, M. A. S. V., and Souza, E. B. 2011. Polyphasic characterization of pigmented strains of Xanthomonas pathogenic to cashew trees. Plant Dis. 95:793-802.The export of cashew {Anacardium occidentale) nuts generates millions of dollars for the Brazilian economy annually. However, production may be limited by the occurrence of diseases that affect cashew trees, such as Xanthomonas spot and angular leaf spot, which are caused by pigmented strains of Xantlummim.s and Xanthomonas ci tri pv. anacardii, respectively. Thirty-one pigmented strains o'i Xanthomonas were characterized for phenotypic, pathogenic, and molecular attributes. These strains were similar to X. citri pv. anacardii in phenotypical characteristics, sensitivity to antibiotics and copper compounds used in agriculture, epidemiology, and repetitive sequence-based polymerase chain reaction (rep-PCR) profiles. When inoculated into Brazilian pepper, cashew, mango, and hog plum seedlings, the pigmented strains of Xanthomonas and X. citri pv. anacardii produced similar symptoms. However, the pigmented strains of Xanthomonas were more aggressive toward cashew plants than toward the other hosts tested, which confirms their specificity. We conclude that pigmented strains oí Xanthomonas are very aggressive on cashew trees and should not be considered casual pathogens of these hosts. Moreover, based on our results from rep-PCR and IS/595-PCR amplification, we suggest that these strains constitute a variant of X. citri pv. anacardii.The production of cashew nuts (Anacardium occidentale L.) in Brazil is traditionally intended for the international market (14). In 2(K)7, exports of cashews ready for consumption reached 51 thousand tons, generating approximately 225 million U.S. dollars for the Brazilian economy (6). This production may be compromised by the many diseases aflecting cashew trees, including Xanthomonas spot caused by Xanthomonas campestris pv. niangiferaeindicae (Patel et al.) Robbs et al. (pigmented strains oí Xanthomonas) (29-32) and angular leaf spot caused by Xanthomonas citri pv. anacardii (Patel et al.) Ah-You et al. (1,2).X. campestris pv. mangiferaeindicae was originally reported causing mango (Mangifera indica L.) bacterial black spot in South Africa hy Doidge (5), and was natned Bacillus mangijera Doidge. In 1948, similar sytnptoms were ob.served in mango leaves and fruit in India by Patel et al. (16,17), who proposed the creation of a new species, Pseudomonas mangiferae-indicae Patel et al., because of differences observed between these strains and those from South Africa. Additionally, the authors described the pathogenicity of this bacterium in cashew trees without any further reports on its occurrence in nature or studies on its pathosysteni. In 1974, this species was renatned Xanthomonas campestris pv. mangiferaeindicae by Robbs ef al. (23). Interestingly, while both pigmented and nonpigmented strains of this bacterium have been isolated from mango trees with symptoms of bacterial bl...

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Section: Methodsmentioning

confidence: 99%

Polyphasic Characterization of Pigmented Strains of Xanthomonas Pathogenic to Cashew Trees

et al. 2011

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“…Infere-se que o patógeno é capaz de sobreviver em altas populações em restos de poda de videira infectados durante, pelo menos, 80 dias do ciclo da cultura, constituindo fonte de inóculo primário para plantas sadias. Nascimento et al (2005) observaram que Xcv sobreviveu até 150 dias em folhas de videira herborizadas mantidas à temperatura ambiente de laboratório (25±2°C Rif (Figura 1), que pode estar associada às condições ambientais, pois durante este período a temperatura máxima do ar e do solo foi, em média, 34,2 ±1,5°C e 46,5 ±6,5°C. Jones et al (1986), trabalhando com X. campestris pv.…”

Section: Sobrevivência De Xcv2unclassified

Sobrevivência de Xanthomonas campestris pv. viticola em tecido infectado de videira

Silva¹,

Menezes

Souza³

et al. 2012

Rev. Bras. Frutic.

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RESUMO -Tecidos de plantas infectados constituem uma importante fonte de inóculo primário para fitobacterioses. O objetivo deste trabalho foi investigar a sobrevivência de Xanthomonas campestris pv. viticola (Xcv), agente do cancro bacteriano da videira, em tecidos infectados na superfície do solo e durante a compostagem de restos de poda. Mudas de videira 'Festival' foram inoculadas com mutante resistente à rifampicina Xcv2Rif e mantidas em casa de vegetação até apresentarem alta severidade da doença. No primeiro experimento, ramos fragmentados e folhas inteiras destas plantas foram acondicionados em bolsas de malha plástica e colocados na superfície de microparcelas. No segundo experimento, ramos e folhas fragmentados foram acondicionados em bolsas plásticas e depositados no interior de pilhas de compostagem de restos de poda de videira. A sobrevivência de Xcv2Rif em tecidos infectados foi monitorada a intervalos de 8 e 10 dias a partir do início do primeiro e segundo experimentos, respectivamente. No primeiro experimento, foi também avaliada a decomposição de tecidos, e no segundo, as curvas de temperatura das pilhas, conteúdo de fenóis e microbiota antagonista a Xcv2 Rif. O patógeno sobreviveu em altas populações até 80 dias em tecidos infectados na superfície do solo. A compostagem eliminou Xcv2Rif de restos culturais em 10 dias, devido às altas temperaturas, liberação de compostos fenólicos e antagonismo microbiano. Concluindo, em tecidos infectados de videira Xcv sobrevive na superfície do solo por, pelo menos, 80 dias mas é eliminada pela compostagem em 10 dias. Termos para Indexação: Cancro bacteriano da videira, restos de cultura, epidemiologia, Xanthomonas campestris pv. viticola, erradicação. SURVIVAL OF Xanthomonas campestris pv. viticola IN INFECTED TISSUES OF GRAPEVINEABSTRACT -Infected plant tissues are an important primary inoculum sources for plant bacterial diseases. This study investigated the survival of Xanthomonas campestris pv. viticola (Xcv), the agent of bacterial canker of grapevine in infected tissues on soil surface and during composting process of pruning residues. Grapevine plants cv. Festival were inoculated with a mutant resistant to rifampicin Xcv2 Rif and maintained under greenhouse conditions until presenting high disease severity. In the first experiment, fragmented shoots and entire leaves from these plants, were placed into mesh bags and located on the surface of microplots. In the second experiment fragmented shoots and leaves were also placed into mesh bags and located inside composting piles of grapevine pruning residues. The survival of Xcv2Rif in infected tissue was monitored at 8 and 10 days intervals from 1 and 2 experiment setting, respectively. In the experiment 1 tissue decomposition was also evaluated and in the experiment 2, pile temperature curves, phenolyc content, and antagonism against Xcv2Rif were analyzed. The pathogen survived in high populations for at least 80 days in grapevine-infected tissues on soil surface. The composting process eliminated Xcv2Rif ...

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“…Usually plant pathogenic bacteria grow under temperatures ranging from 25ºC to 40ºC (mesophilic bacteria), since higher temperatures cause desiccation in the bacteria cells (Bedendo, 1995); pH close to neutrality (6.5 to 7.5); and salt concentration no above 5% NaCl. Nascimento et al (2005) for instance, reported that the phytopathogenic Xanthomonas campestris pv. viticola decreased after 3.0% of NaCl, being 6.0% a lethal concentration.…”

Section: Discussionmentioning

confidence: 99%

Facultative Extremophilic Bacteria in Brazilian Agricultural Soils

Rezende

Uesugi

Jesus

et al. 2020

BJD

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The objective was to study different groups of bacteria that can adapt to extreme conditions of temperature, pH and salinity, as well as to identify bacterial strains from cultivated (CS) and noncultivated (US) soil samples collected in the city of Brazlândia, Brazil. 38 strains were collected and submitted to growth tests at 45, 55, 65 and 75ºC, pH of 3, 4, 5, 9, 10 and 11 and salinity of 5, 10 and 15% of NaCl. Eleven strains were selected based on their biochemical characterization and identified based on their 16S rDNA sequences. Four groups of bacteria in the CS and US samples were identified and able to grow under the most extreme conditions. Eleven bacterial strains were

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Métodos de preservação e crescimento de Xanthomonas campestris pv. viticola em meio de cultura variando temperatura, pH e concentração de NaCl

Cited by 6 publications

References 2 publications

Polyphasic Characterization of Pigmented Strains of Xanthomonas Pathogenic to Cashew Trees

Polyphasic Characterization of Pigmented Strains of Xanthomonas Pathogenic to Cashew Trees

Sobrevivência de Xanthomonas campestris pv. viticola em tecido infectado de videira

Facultative Extremophilic Bacteria in Brazilian Agricultural Soils

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