Sobrevivência e viabilidade de Xanthomonas axonopodis pv. phaseoli em sementes de feijão armazenadas sob condições controladas

Marques, Ana Maria Campos; Guimarães, P. M.; Santos, J.P.; Vieira, Tatiana Martins

doi:10.1590/s0100-41582005000500012

Cited by 9 publications

(3 citation statements)

References 6 publications

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“…phaseoli was reduced from 64% to 36% to 37% incidence during the first 6 months; however, seed stored at -18°C and 5°C maintained the contamination rate at 30 and 60 months, and it was concluded that the optimum temperatures for storing seed is similar to conditions favourable for Xap longevity [63]. Seed contamination may be internal or external [64] and even symptomless, which has serious implications for seed certification schemes.…”

Section: Transmissionmentioning

confidence: 99%

Common Bacterial Blight (Xanthomonas axonopodis pv. phaseoli) of Beans with Special Focus on Ethiopian Condition

Baştaş¹,

Kk²

2017

J Plant Pathol Microbiol

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Common bacterial blight (CBB) is the most devastating factor that affects common bean crops in all common bean growing areas. This review was to review with an objective of reviewing the biology, economic importance of CBB of common bean crop disease and its management options, with an emphasis on the future research direction and priorities. CBB disease, caused by the gram-negative bacterial pathogen Xanthomonas axonopodis pv. phaseoli (Xap) and its fuscans variant Xanthomonas fuscans subsp. fuscans (Xff) is the major bottleneck in bean production in the world as well as in Ethiopia. It is a serious bacterial disease of common bean which causes lesions on the leaves, stems, pods and seeds of the plant. The disease affects seed quality and can reduce yield by up to 45%, may be more in susceptible cultivars. CBB is very difficult to control due to seed-borne nature of the bacteria and its capacity to produce huge amounts of secondary inoculum. Since the disease is very important in causing economic losses of yields on bean crop, developing and using effective and appropriate management options is unquestionable. Using resistant varieties supplemented with chemical seed treatment and proper cultural practices could be the best alternative options in managing common bacterial blight of common bean and avoiding yield loss. In general, integrated disease management is the preferred strategy because of increased understanding on residual effects of chemical control on non-target organisms and environment as well as the limitation of a single alternative management option to achieve the same level of control and reliability as that of chemical. In the case of Ethiopia, emphasis should be given to developing multi line resistance varieties by suitable breeding practice and developing molecular markers to enhance marker assisted selection.

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Section: Transmissionmentioning

confidence: 99%

Common Bacterial Blight (Xanthomonas axonopodis pv. phaseoli) of Beans with Special Focus on Ethiopian Condition

Baştaş¹,

Kk²

2017

J Plant Pathol Microbiol

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“…Prema Taylor (1978) imunofluorescencijom je moguće detektovati 10 3 bakterija u 1 ml, dok drugi autori navode detekciju 80 ćelija u 1 ml macerata semena . Marques et al (2005) su prilikom izvođenja DAS ELISA testa za identifikaciju izolata Xap dobijenih sa semena pasulja koristili suspenziju bakterije različitih koncentracija, od 10 8 do 10 3 cfu/ml. Roth (1988) je za detekciju Xap na semenu pasulja razvio proceduru koja uključuje imunofluorescenciju (IF), na osnovu koje je moguće detektovati jedno zaraženo seme u partiji od 10.000.…”

Section: Rezultatiunclassified

Detection of Xanthomonas axonopodis pv. Phaseoli and Pseudomonas savastanoi pv. Phaseolicola on bean seed using a Milk-tween medium

Popović¹,

Ignjatov²,

Jošić³

et al. 2012

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Izvod: Proizvodnju pasulja ugrožavaju fitopatogene bakterije prouzrokovači plamenjača Xanthomonas axonopodis pv. phaseoli (Xap) i Pseudomonas savastanoi pv. phaseolicola (Psp). S obzirom da do sada nije postignuta zadovoljavajuća hemijska zaštita za suzbijanje ovih bakterija, mere koje se preporučuju preventivnog su karaktera i uključuju upotrebu zdravog semena za setvu, plodored, duboko zaoravanje ostataka i korišćenje otpornih sorti. U ovom radu je vršena detekcija Xap i Psp na semenu pasulja metodom izolacije na poluselektivnu podlogu Milk Tween Agar (MT). Na ovoj podlozi Xap obrazuje žute, sluzaste i ispupčene kolonije sa dve zone hidrolize (manjim mlečnim i većim prosvetljenim), a Psp obrazuje kolonije beličasto-krem, ravne i okrugle. Identifikacija izolata Xap i Psp je potvrđena primenom ELISA i PCR. Podloga MT se zbog svoje selektivnosti, jednostavne pripreme i mogućnosti istovremene detekcije bakterija Xap i Psp može preporučiti prilikom rutinskog ispitivanja zdravstvenog stanja semena pasulja za setvu proizvedenog kod nas ili prilikom kontrole semena iz uvoza. Ključne reči: bakterije, pasulj, Pseudomonas, seme, Xanthomonas UvodUsled česte pojave i jakog intenziteta, bakterioze pasulja mogu prouzrokovati znatne gubitke čime se svrstavaju u ekonomski značajne bolesti ove biljne vrste (Tešić 1946, Zaumeyer & Thomas 1957, Кiryakov 1999, Schwartz 2004, Popović 2008. U našim uslovima gajenja pasulja bakterije Xanthomonas axonopodis pv. phaseoli (Smith 1897) Vauterin et al. 1995 (Xap) i Pseudomonas savastanoi pv. phaseolicola (Burkholder 1926) Gardan et al. 1992, prouzrokovači plamenjača imaju najveći ekonomski značaj.Zaraženo seme je osnovni izvor inokuluma, pa se uvozu i prozvodnji semena pasulja i boranije mora posvetiti velika pažnja. Epidemiološke studije su pokazale da jedno zaraženo seme bakterijom Xap u 10.000 (Sutton & Wallen 1970) ili jedno seme zaraženo sa Psp u 2.000 ili 5.000 može rezultovati epifitocijama u polju (Trigalet & Bidaud 1978, Webster et al. 1983. Zaraženo seme pasulja može biti sa i bez vidljivih simptoma (latentne infekcije) ili simptomi mogu biti prikriveni simptomima drugih oboljenja (Leben 1981). Detekcija bakterija na semenu zahteva ekstrakciju bakterija iz semena, a zatim njihovu identifikaciju korišćenjem raznih dijagnostičkih testova. Iako je opisano nekoliko metoda za utvrđivanje fitopatogenih bakterija na semenu, a uključuju serologiju, korišćenje bakteriofaga ili molekularnu dijagnostiku, izolacija na poluselektivne podloge predstavlja najčešće korišćen metod za ovakva ispitivanja (Schaad 1982). Standardne laboratorijske procedure zasnovane na izolacijama na poluselektivne podloge opisane su od strane International Seed Testing (ISF 2006a(ISF , 2006b), International Seed Testing Association, ISTA (Remeeus & Sheppard 2006, Sheppard et al. 2007, 2008 i National Seed Health System (NSHS 2002). Od nekoliko poluselektivnih podloga koje su opisane original scientific paper / originalni naučni članak www.nsseme.com/journal.html Zahvalnica: Ovaj rad je deo istraživanja na proje...

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“…A bactéria Xanthomonas axonopodis pv. phaseoli pode sobreviver por períodos de até 5 anos em sementes de feijão, quando armazenada em condições adequadas, mantendo as populações bacterianas elevadas e com capacidade de causar doença em plântulas emergentes (Marques et al, 2005 Brasil e atualmente encontra-se disseminada em todas as regiões produtoras do mundo (Romero, 2005).…”

Section: Transmissão Do Patógenounclassified

Curtobacterium flaccumfaciens pv. flaccumfaciens: etiologia,detecção e medidas de controle

2010

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ResumoAs doenças de etiologia bacteriana são problemas fi topatológicos e agronômicos em várias culturas de interesse econômico. A Murcha de Curtobacterium causada pela Curtobacterium fl accumfaciens pv. fl accumfaciens tem se tornado uma ameaça ao cultivo do feijão (Phaseolus vulgaris L.). Sua constatação no território brasileiro ocorreu em 1995 e hoje o patógeno encontra-se distribuído em várias regiões produtoras de feijão do país. A transmissão ocorre principalmente via sementes contaminadas, o que impõe grandes difi culdades e limitações para a contenção desta bactéria, sendo a erradicação das plantas a única forma de controle pós-infecção. As alternativas viáveis para o controle desta doença são: a utilização de sementes sadias, a rotação de culturas e o uso de variedades resistentes, sendo este último, um dos métodos mais efi ciente e economicamente viável para o produtor. Estudos têm sido desenvolvidos para dar suporte ao desenvolvimento de cultivares resistentes à bactéria C. fl accumfaciens pv. fl accumfaciens, dentre os quais destacam-se a identifi cação de fontes de resistência genética à bactéria e o estudo dos mecanismos de herança genética da resistência à doença. Unitermos: bacteriose, Murcha de Curtobacterium, Phaseolus vulgaris L. AbstractCurtobacterium fl accumfaciens pv. fl accumfaciens: etiology, detection and control strategies. The diseases caused by bacteria present a phytopathological and agricultural problem in various economic crops. The Curtobacterium wilt caused by Curtobacterium fl accumfaciens pv. fl accumfaciens has become a threat to the cultivation of beans (Phaseolus vulgaris L.). The disease statement for the Brazilian territory occurred in 1995 and today the pathogen is distributed in various Brazilian producing regions. Transmission is primarily via contaminated seed, which imposes great diffi culties and limitations on the control of these bacteria. After plant infection, the only way to control it is the eradication of infected plants. The alternatives for the control of this disease are the use of healthy seeds, crop rotation and resistant varieties. The use of resistant varieties is the most effi cient and economical solution for the farmer. The most important research to support the development of bean cultivars resistant to C. fl accumfaciens pv. fl accumfaciens is the identifi cation of sources of genetic resistance and the study of mechanisms of genetic resistance to disease.

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Sobrevivência e viabilidade de Xanthomonas axonopodis pv. phaseoli em sementes de feijão armazenadas sob condições controladas

Cited by 9 publications

References 6 publications

Common Bacterial Blight (Xanthomonas axonopodis pv. phaseoli) of Beans with Special Focus on Ethiopian Condition

Common Bacterial Blight (Xanthomonas axonopodis pv. phaseoli) of Beans with Special Focus on Ethiopian Condition

Detection of Xanthomonas axonopodis pv. Phaseoli and Pseudomonas savastanoi pv. Phaseolicola on bean seed using a Milk-tween medium

Curtobacterium flaccumfaciens pv. flaccumfaciens: etiologia,detecção e medidas de controle

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