Genes diferentes podem conferir resistência ao Cowpea severe mosaic virus em caupi

Assunção, Iraíldes Pereira; M.-Filho, Liliane R.; Resende, Luciane Vilela; Barros, Márcia C. S.; Lima, Gaus Silvestre de Andrade; Coelho, Rildo Sartori Barbosa; Lima, J.A.

doi:10.1590/s0100-41582005000300009

Cited by 5 publications

(11 citation statements)

References 6 publications

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“…The inoculated cowpea genotypes were tested by RT-PCR using universal primers for the genus Comovirus: F, 5'-GCATGGTCCACWCAGGT-3' and R, 5'-YTCRAAWCCVYTRTTKGGMCCACA-3' (Camarço et al, 2009). Total RNA was extracted from leaf samples of inoculated plants, using the protocol developed by Rott & Jelkmann (2001), with some modifications.…”

Section: Methodsmentioning

confidence: 99%

“…The North and Northeast of Brazil are the largest producer areas, and the state of Ceará is considered the greatest producer in the country (Freire Filho et al, 2005;Lima et al, 2005b). Therefore, this leguminous crop is of strategic importance for small farmers and an option for the agribusiness of the North and Northeast of Brazil.…”

Section: Introductionmentioning

confidence: 99%

“…The development of resistant cultivars has been universally considered the most effective method to control diseases caused by viruses in cowpea, indicating that an increase in the number of virus-resistant genotypes will generate more alternatives for breeders to produce resistant cultivars (Lima et al, 1986(Lima et al, , 2005aHampton et al, 1997;Assunção et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

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Simple and multiple resistances to viruses in cowpea genotypes

Lima

Silva

Aragão

et al. 2011

Pesq. agropec. bras.

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-The objective of this work was to identify new sources of simple and multiple resistances to Cowpea severe mosaic virus (CPSMV), Cowpea aphid-borne mosaic virus (CABMV) and Cucumber mosaic virus (CMV) isolates in cowpea (Vigna unguiculata). Thirty-three genotypes from the germplasm bank of Universidade Federal do Ceará were tested as to their resistance to four CPSMV isolates, two CABMV isolates and one CMV isolate. Twenty-five days after the first virus inoculations, all inoculated plants, including the asymptomatic ones, were tested by serology. Genotypes were classified as: immune, plants without symptoms and negative serology; resistant, plants with mild mosaic and positive serology; susceptible, plants with mosaic and positive serology; and highly susceptible, plants with severe mosaic, other systemic symptoms, including systemic necrosis, and positive serology. Simple and multiple resistances to viruses were identified among the evaluated genotypes, but none of them showed multiple immunities to all isolates. Four genotypes showed immunity to all CPSMV isolates, two were immune to CABMV and two showed immunity to CMV. Eleven genotypes showed multiple resistances to two viruses, allowing for the development of new cultivars with more stable and broader resistance. Genotypes Purple Knuckle Hull-55, MNC-03-731C-21 and CNCx284-66E show resistance to CABMV, even when inoculated with CMV.Index terms: Cowpea aphid-borne mosaic virus, Cowpea severe mosaic virus, Cucumber mosaic virus, Vigna unguiculata, serology, virus control. Resistência simples e múltipla a vírus em genótipos de caupi Resumo -O objetivo deste trabalho foi identificar novas fontes de resistência simples e múltiplas em genótipos de feijoeiro caupi (Vigna unguiculata) a isolados de Cowpea severe mosaic virus (CPSMV), Cowpea aphid-borne mosaic virus (CABMV) and Cucumber mosaic virus (CMV). Trinta e três genótipos do Banco de Germoplasma daUniversidade Federal do Ceará foram classificados quanto a sua resistência a quatro isolados de CPSMV, dois de CABMV e um de CMV. Após 25 dias da primeira inoculação dos isolados, todas as plantas que sofreram inoculação, incluindo as assintomáticas, foram testadas por meio de sorologia. Os genótipos foram classificados como: imune, plantas assintomáticas e sorologia negativa; resistentes, plantas com mosaico leve e sorologia positiva; suscetível, plantas com mosaico e sorologia positiva; altamente suscetível, plantas com mosaico severo, outros sintomas sistêmicos, como necrose sistêmica, e sorologia positiva. Foram identificadas fontes de resistência simples e múltipla às viroses, nos genótipos avaliados; no entanto, nenhum deles apresentou imunidade múltipla aos três vírus. Quatro genótipos apresentaram imunidade aos isolados de CPSMV, dois aos isolados de CABMV e dois ao CMV. Onze genótipos apresentaram resistência múltipla a dois vírus, o que possibilita o desenvolvimento de novas cultivares com resistência mais abrangente e estável. Os genótipos Purple Knuckle Hull-55, MNC-03-731C-21 e CNCx284-66E m...

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Simple and multiple resistances to viruses in cowpea genotypes

Lima

Silva

Aragão

et al. 2011

Pesq. agropec. bras.

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“…Several bean and tomato cultivars were developed and occupied significant acreages due to different traits such as fruit setting at high temperatures, fruit firmness and resistance to several diseases (Queiroz and Lopes 2007), attributes essential for a successful crop in the Northeast. IPA maintains germplasm collections of cowpea (Assunção et al 2005), sweet potato (Ferreira et al 2005). …”

Section: Brazilian Germplasm Collections -A Snapshotmentioning

confidence: 99%

Genetic resources: the basis for sustainable and competitive plant breeding

Nass

Sigrist

Ribeiro

et al. 2012

Crop Breed. Appl. Biotechnol.

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“…In the state of Piauí, northeastern Brazil, only serotypes I and II were identified (Santos, 1990). CPSMV control strategies are essentially based on the growth of resistant cowpea genotypes, such as Macaibo, CNC-0434 (Assunção et al, 2005), BR-10 Piauí (Santos et al, 2000), and BR 17 (Oliveira et al, 2012). CABMV belongs to the Potyvirus genus, Potyviridae family (Adams et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Selection of phenotypic traits and resistance to Cowpea severe mosaic virus and Cowpea aphid-borne mosaic virus in cowpea [Vigna unguiculata (L.) Walp.] seeds with rugose white coat

Leão¹,

Eiras²,

Filho³

et al. 2016

Aust J Crop Sci

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Cowpea [Vigna unguiculata (L.) Walp.] is an important protein source in diets in Brazil. The preference of consumers is for large, white, light brown and evergreen seeds with rugose coat and small hilum and hilum ring. The Cowpea severe mosaic virus (CPSMV) and the Cowpea aphid-borne mosaic virus (CABMV) are the main pathogens in cowpea plantations in Brazil. This study selected cowpea offspring with (i) white rugose coat seed characteristics that are accepted by markets and (ii) that present resistance to CPSMV (serotype I) and CABMV. The first selection of asymptomatic plants was carried out using seedlings from F 3 seeds mechanically inoculated with a mixture of the two viruses. Offspring F 3:4 went through a second selection process with two stages, one in the field (with natural inoculation), one in trays (with mechanical inoculation). In total, 40 F 3:4 offspring were selected to evaluate agronomic traits in two field assays, one in Teresina, one in Tracuateua (states of Piauí and Pará, respectively) based on a randomized block design with four repetitions. Significant effect of genotype and of the interaction assay x genotype was observed (p ≤ 0.01) for most of the traits evaluated: weight of 100 seeds, yield, seed length, width, and height; length-to-height ratio; width-toheight ratio, and hilum width-to-length ratio. Fourteen offspring produced large seeds (25 -30 g in 100 seeds), and four presented cross-resistance to CPSMV and CABMV, while 36 offspring were resistant only to CPSMV.

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Genes diferentes podem conferir resistência ao Cowpea severe mosaic virus em caupi

Cited by 5 publications

References 6 publications

Simple and multiple resistances to viruses in cowpea genotypes

Simple and multiple resistances to viruses in cowpea genotypes

Genetic resources: the basis for sustainable and competitive plant breeding

Selection of phenotypic traits and resistance to Cowpea severe mosaic virus and Cowpea aphid-borne mosaic virus in cowpea [Vigna unguiculata (L.) Walp.] seeds with rugose white coat

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