Detecção por DAS-ELISA do Apple chlorotic leaf spot virus em pétalas desidratadas de macieira mantidas em geladeira por 16 anos

Abstract

DAS-ELISA detection of Apple chlorotic leaf spot virus in dehydrated apple petals stored under refrigeration for 16 years Flower petals of eleven apple (Malus domestica) clones doubly infected with Apple chlorotic leaf spot virus (ACLSV) and Apple stem grooving virus (ASGV) were harvested in 1985, dehydrated in calcium chloride and stored in the refrigerator at 4-5 o C. Petals from fourteen virus free clones were also stored at the same time. DAS-ELISA with antisera against both viruses detected ACLSV in petals from eight clones after sixteen years of storage. ASGV was not detected in any clone. All virus free clones tested negative for both viruses.