Colonização de raízes de plantas daninhas cultivadas in vitro e em vasos por Ralstonia solanacearum, biovares 1, 2 e 3

Miranda, Edin Francisco Orozco; Takatsu, A.; Uesugi, Carlos Hidemi

doi:10.1590/s0100-41582004000200001

Cited by 7 publications

(4 citation statements)

References 11 publications

(8 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The sterilized seeds were germinated in the dark for 48h on Petri dish containing agar gel (0.7%). The germinated seeds were treated with the Actinomycetota inoculums (P16, P18, BC3, BC10 and BC11; at 10 8 CFU ml -1 ) for 12h, sown in the pots containing sterilized coarse sand, and incubated in a growth chamber for 15 days ( Bringel, 1997 ; Miranda, 1997 ). At the end of the incubation, wheat seedlings were removed carefully from the pots and the roots were washed in 0.1 M phosphate buffer (pH 7.2).…”

Section: Methodsmentioning

confidence: 99%

Improving the efficiency of phosphate rocks combined with phosphate solubilizing Actinomycetota to increase wheat growth under alkaline and acidic soils

et al. 2023

View full text Add to dashboard Cite

Low availability of phosphorus (P) in both acidic and alkaline soils is a major problem for sustainable improvement in wheat crops yield. Optimization of crops productivity can be achieved by increasing the bioavailability of P by phosphate solubilizing Actinomycetota (PSA). However, their effectiveness may vary with changing agro-climatic conditions. In this regard, a greenhouse experiment was conducted to assess the interaction inoculation of five potential PSA (P16-P18-BC3-BC10 and BC11) and RPs (RP1- RP2-RP3 and RP4) on the growth and yield of wheat crop in unsterilized P- deficient alkaline and acidic soils. Their performance was compared with single super phosphate (TSP) and reactive RP (BG4). The in-vitro tests showed that all PSA colonize wheat root and form a strong biofilm except Streptomyces anulatus strain P16. Our findings revealed that all PSA significantly improve the shoot/root dry weights, spike biomass, chlorophyll contents as well as nutrients uptake in plants fertilized with RP3 and RP4. However, the combined application of Nocardiopsis alba BC11 along with RP4 in alkaline soil, was effective in optimizing wheat yield attributes and improve the yield biomass up to 19.7% as compared to the triple superphosphate (TSP). This study supports the view that the inoculation with Nocardiopsis alba BC11 has a broad RP solubilization and could alleviate the agricultural losses due to P limitation in acidic and alkaline soils.

show abstract

Section: Methodsmentioning

confidence: 99%

Improving the efficiency of phosphate rocks combined with phosphate solubilizing Actinomycetota to increase wheat growth under alkaline and acidic soils

et al. 2023

View full text Add to dashboard Cite

show abstract

“…In Brazil, species of Lepidium virginicum, Nicandra physalodes, S. americanum, Portulaca oleracea, Physalis angulata, Amaranthus spp., Euphorbia heterophylla, Crotalaria spectabilis and B. pilosa are listed as potential hosts to Ralstonia spp. (Miranda et al 2004). Even though the tests were conducted under controlled conditions there is evidence that weeds can be an important inoculum source to the forthcoming crops in the same area.…”

mentioning

confidence: 99%

“…These species have been reported as plants that allow the survival of Ralstonia spp. (Tusiime et al 1998;Miranda et al 2004;2009). Ralstonia spp.…”

mentioning

confidence: 99%

On the assessment of the sources of inoculum of bacterial wilt in Brazil

Mizubuti

Yamada

Santiago

et al. 2022

Trop. plant pathol.

View full text Add to dashboard Cite

Dispersal of Ralstonia spp. cells by water and contaminated plant material and the importance of weeds as inoculum sources have been poorly investigated. Water of rivers, soil from fields of diverse crops and areas of natural vegetation both from the Amazônia, Cerrado and Mata Atlântica biomes, besides soil of the rhizosphere of weeds present in tomato fields with records of bacterial wilt were sampled and analyzed to detect Ralstonia spp. Seeds of tomato plants artificially and naturally infected with Ralstonia spp. were also processed. All samples were enriched a priori in selective medium South Africa (SMSA) and colonies were isolated in plates containing solid SMSA. Detection of Ralstonia spp. was confirmed by polymerase chain reaction with specific primers. The Co -operational PCR (CO-PCR) was also used to detect Ralstonia spp. Colonies were obtained from soil samples and from a commercial substrate sample. Five soil samples from eggplant fields, one from coffee field, one substrate from potato seed tuber production, two soil samples from the rhizosphere of Amaranthus spp., one from Bidens pilosa and one from Solanum americanum tested positive for Ralstonia spp. Besides these soil samples, five water samples of rivers were positive for CO-PCR detection: two samples from Amazônia, one from Cerrado and two samples from irrigation water collected from tomato fields located in the Mata Atlântica biome..

show abstract

“…These species have been reported as plants that allow the survival of Ralstonia spp. (Tusiime et al 1998;Miranda et al 2004;. Ralstonia spp.…”

mentioning

confidence: 99%

On the assessment of the sources of inoculum of bacterial wilt in Brazil

Mizubuti

Yamada

Santiago

et al. 2021

Preprint

View full text Add to dashboard Cite

Dispersal of Ralstonia spp. cells by water and contaminated plant material and the importance of weeds as inoculum sources have been poorly investigated. Water of rivers, soil from fields of diverse crops and areas of natural vegetation both from the Amazonia, Cerrado and Mata Atlantica biomes, besides soil of the rhizosphere of weeds present in tomato fields with records of bacterial wilt were sampled and analyzed to detect Ralstonia spp. Seeds of tomato plants artificially and naturally infected with Ralstonia spp. were also processed. All samples were enriched a priori in selective medium South Africa (SMSA) and colonies were isolated in plates containing solid SMSA. Detection of Ralstonia spp. was confirmed by polymerase chain reaction with specific primers. The Co-operational PCR (CO-PCR) was also used to detect Ralstonia spp. Colonies were obtained from soil samples and from a commercial substrate sample. Five soil samples from eggplant fields, one from coffee field, one substrate from potato seed tuber production, two soil samples from the rhizosphere of Amaranthus spp., one from Bidens pilosa and one from Solanum americanum tested positive for Ralstonia spp. Besides these soil samples, five water samples of rivers were positive for CO-PCR detection: two samples from Amazonia, one from Cerrado and two samples from irrigation water collected from tomato fields located in the Mata Atlantica biome. Ralstonia spp. were not detected in tomato seeds. These results revealed potential inoculum sources, especially weeds, in areas with historical records of bacterial wilt. Additionally, rivers may act as dispersal agents of inoculum of Ralstonia spp.

show abstract

Colonização de raízes de plantas daninhas cultivadas in vitro e em vasos por Ralstonia solanacearum, biovares 1, 2 e 3

Cited by 7 publications

References 11 publications

Improving the efficiency of phosphate rocks combined with phosphate solubilizing Actinomycetota to increase wheat growth under alkaline and acidic soils

Improving the efficiency of phosphate rocks combined with phosphate solubilizing Actinomycetota to increase wheat growth under alkaline and acidic soils

On the assessment of the sources of inoculum of bacterial wilt in Brazil

On the assessment of the sources of inoculum of bacterial wilt in Brazil

Contact Info

Product

Resources

About