Identificação e caracterização de um potyvírus isolado de Zinnia elegans

Maritan, Ana C; Gaspar, José Carlos; Camargo, Luís Eduardo Aranha

doi:10.1590/s0100-41582004000100005

Cited by 11 publications

(10 citation statements)

References 17 publications

(13 reference statements)

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“…The deduced aa sequence was compared with the SuCMoV CP sequence, sharing 96% aa identity. Also, the CP sequence of the virus isolated from D. fullonum shared 90% aa identity with the SuCMoV-Zi strain isolated from Zinnia elegans in Brazil (SuCMoV-Zi: AY344048, Maritan et al, 2004). A high identity value was found, too, with Bidens mosaic virus (BiMV: AY960151) 85%, Potato virus Y (PVY: AJ303095) 81%, PVY-NsNr: (X68224) 81%, Pepper mottle virus (PepMoV: M11598) 80% and Pepper severe mosaic virus (PepSMV: X66027) 78%.…”

Section: Resultsmentioning

confidence: 96%

Dipsacus fullonum: an Alternative Host of Sunflower chlorotic mottle virus in Argentina

Giolitti¹,

Bejerman²,

Lenardon³

2009

Journal of Phytopathology

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Dipsacus fullonum L. is a biennial weed that is widely spread in the Pampean region of Argentina. Plants of this species showing leaf mosaic symptoms were collected near sunflower (Helianthus annuus L.) crops infected with Sunflower chlorotic mottle virus (SuCMoV). Symptomatic plants were tested by biological, serological and molecular assays. Sunflower and Nicotiana occidentalis L. plants mechanically inoculated with extracts from symptomatic D. fullonum leaves developed typical SuCMoV symptoms. Samples were DAS-ELISA positive when probed with a SuCMoV antiserum. RT-PCR was used to amplify the whole coat protein gene of the infecting virus. An 807 bp fragment was amplified, cloned and sequenced. The deduced amino acid sequence (EU606023) shared 96% amino acid identity with the SuCMoV sequence (AF255677). These results indicate that D. fullonum mosaic is caused by an isolate of SuCMoV. Thus, this finding has epidemiological relevance as the weed may act as a natural virus reservoir.

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Section: Resultsmentioning

confidence: 96%

Dipsacus fullonum: an Alternative Host of Sunflower chlorotic mottle virus in Argentina

Giolitti¹,

Bejerman²,

Lenardon³

2009

Journal of Phytopathology

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“…Para obtenção de sementes com qualidade superior devem ser conhecidas as modificações que ocorrem nos teores de água, no conteúdo de matéria seca, na germinação e no vigor durante o seu desenvolvimento (Sader e Silveira, 1988), ou seja, definir a época de maturação fisiológica ideal para colheita das sementes. Além disso, em várias partes do mundo, zínia foi descrita como hospedeira natural ou experimental de patógenos (Maritan et al, 2004). Portanto, suas sementes merecem uma especial atenção no que se refere à avaliação de sua qualidade fisiológica e sanitária.…”

Section: Introductionunclassified

Envelhecimento acelerado em sementes de Zinnia elegans Jacq. colhidas em diferentes épocas

Menezes

Pedroso²,

Muniz

et al. 2008

Rev. bras. sementes

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RESUMO -O objetivo do trabalho foi utilizar o teste de envelhecimento acelerado para avaliar o potencial fisiológico de sementes de Zinnia elegans Jacq., cultivar Gigante da Califórnia, colhidas em três diferentes épocas, bem como identificar os gêneros de fungos associados às sementes e sua influência sobre a qualidade das mesmas. Utilizaram-se sementes colhidas em janeiro, fevereiro e março de 2005, as quais foram submetidas ao envelhecimento acelerado em incubadora tipo BOD a 40ºC por 0, 24, 48, 72 e 96 horas. A qualidade fisiológica foi avaliada pelos testes de germinação, primeira contagem, comprimento de plântula, emergência de plântulas em campo e a qualidade sanitária pelo "Blotter Test". O teste de envelhecimento acelerado conduzido à temperatura de 40ºC, por 72 e 96h, foi eficiente para diferenciação da qualidade das sementes de zínia, colhidas em diferentes épocas. O fungo Aspergillus spp., associado às sementes, influenciou negativamente na germinação.Termos para indexação: zínia, vigor, fungos. ACCELERATED AGEING IN Zinnia elegans Jacq. SEEDS HARVESTED AT DIFFERENT TIMES IN DIFFERENT SEASONSABSTRACT -The objective of the work was to determine the time of exposure adequate to the assessment of physiological potential of the seeds of Zinnia elegans Jacq., cultivar "Single mixed", picked in three different epochs, through the accelerated ageing test, as well as identifying genera of fungi associated to the seeds and their influence on the quality of themselves. It was utilized seeds harvested in january, february and march of 2005, which remained in BOD chamber at 40° C for 0, 24, 48, 72 and 96 hours. The physiological quality was evaluated by the tests of germination, first counting, the seedling length, rising of seedling, in field and plant length, and, the sanitary quality by "Blotter Test". The test of accelerated ageing was efficient for the stratification of the quality of the seeds of zinnia harvested in different seasons due to the physiological potential. The periods of the accelerated ageing of 72 and 96 h, at temperature of 40° C, were the ones that demonstrated greater capacity of seed quality physiological differentiation. The fungus Aspergillus spp. associated to the seeds negatively in the germination.Index terms: zinnia, vigor, accelerated aging, fungi. 40 V. O. MENEZES et al.

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“…BiMV-p CP amino acids (Table 1) shared 88% amino acid identity with a Brazilian isolate of SuCMoV-BZ obtained from a zinnia plant [11] and 83% identity with an Argentinian SuCMoV-AR isolate from sunflower [12,13]. Lower identity was found with PVY (82%).…”

mentioning

confidence: 98%

“…The host range of BiMV-p [4], SuCMoV-BZ [11] and SuCMoV-AR [12] were compared. All three isolates showed similar symptoms after infection to sunflower, zinnia and Chenopodium amaranticolor plants, the latter a local lesion host.…”

mentioning

confidence: 99%

Bidens Mosaic Virus is a Member of the Potato Virus Y Species

et al. 2006

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The nucleotide sequence of the genomic 3' terminal region (1,702 bases) of two Brazilian Bidens mosaic virus isolates (BiMV: BiMV-p and BiMV-b) was determined. BiMV-p and BiMV-b share 98% nucleotide sequence identity, and are most closely related to members of the potyvirus species: Sunflower chlorotic mottle virus (an isolate of Potato virus Y) and Potato virus Y. BiMV-p shares 88% capsid protein amino acid identity and 77% 3'UTR nucleotide sequence identity with SuCMoV an isolate of Sunflower chlorotic mottle virus. Phylogenetic analyses suggest the close evolutionary relationship of BiMV, SuCMoV and Potato virus Y (PVY), members of the PVY species. According to the analyses of capsid protein and 3'UTR sequences BiMV isolates must be regarded as a strain of Potato virus Y species, but their differences in host reactions and the phylogenetic distance suggest that they would be most likely better placed in a taxon between species and strains.

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Identificação e caracterização de um potyvírus isolado de Zinnia elegans

Cited by 11 publications

References 17 publications

Dipsacus fullonum: an Alternative Host of Sunflower chlorotic mottle virus in Argentina

Dipsacus fullonum: an Alternative Host of Sunflower chlorotic mottle virus in Argentina

Envelhecimento acelerado em sementes de Zinnia elegans Jacq. colhidas em diferentes épocas

Bidens Mosaic Virus is a Member of the Potato Virus Y Species

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