An isolate of Apple stem grooving virus associated with Cleopatra mandarin fruit intumescence

Abstract

A citrus tatter leaf isolate (CTLV-Cl) of Apple stem grooving virus (ASGV) has been found to be associated with a fruit rind intumescence in Cleopatra mandarin (Citrus reshni) in Limeira (SP). The CTLV-Cl was mechanically transmitted to the main experimental herbaceous hosts of CTLV. Chenopodium quinoa and C. amaranticolor reacted with local lesions and systemic symptoms while other test plants reacted somewhat differently than what is reported for CTLV. A pair of primers designed for specific detection of ASGV and CTLV amplified the expected 801 bp fragment from the CTLV-Cl-infected plants. Typical capillovirus-like particles were observed by the electron microscope in experimentally infected C. quinoa and C. amaranticolor leaves.

Identification and characterization of Apple stem grooving virus causing leaf distortion on pear (Pyrus pyrifolia) in Taiwan

“…The herbaceous host range of ASGV by mechanical inoculation have been reported for apple (Yoshikawa 2000), kiwifruit (Clover et al 2003), citrus (Lovisolo et al 2003) and lily (Inouye et al 1979) isolates, but not for pear isolate. Though ASGV TS1 and previously described isolates differ among themselves in hostpreference, they all could infect C. quinoa, N. glutinosa and P. vulgaris.…”

“…Though ASGV TS1 and previously described isolates differ among themselves in hostpreference, they all could infect C. quinoa, N. glutinosa and P. vulgaris. ASGV isolate TS1 but not citrus isolates could infect A. gangeticus and D. stramonium (Lovisolo et al 2003). D. stramonium showed necrotic local lesions on the inoculated and mild leaf mottling on the systemic leaves when infected by pear TS1 isolate but was symptomless when infected by lily and apple isolates (Inouye et al 1979;Lister et al 1965).…”

“…N. occidentalis and C. sativus were not hosts for TS1 and citrus isolates, but were hosts for kiwifruit isolate (Clover et al 2003). C. pepo could be infected by apple (Uyemoto and Gilmer 1971), citrus (Lovisolo et al 2003), lily (Inouye et al 1979) and TS1 isolates.…”

“…The scale bar representing the genetic distance is indicated at the left bottom (Németh 1986), or cherry (Yoshikawa 2000). In addition, ASGV has been reported to infect citrus (Lovisolo et al 2003;Magome et al 1997), lily (Inouye et al 1979), and kiwifruit (Clover et al 2003). Although most of ASGV isolates cause symptomless latent infection in the commercial apples and pear cultivars (Yoshikawa 2000), several ASGV isolates induce stem grooving, necrosis and deformation on top-grafting unions in susceptible cultivars (Motoshima et al 1983;Németh 1986;Yoshikawa 2000).…”

Apple stem grooving virus naturally infects Himalayan wild cherry (Prunuscerasoides D. Don)

“…It also induces symptoms such as chlorotic leaf spot, brown line above the graft union, and fruit rind intumescence in mandarin (Welsh and Van der Meer ; Yoshikawa ; Lovisolo et al. ).…”