Identificação sorológica do vírus do mosaico das nervuras da videira no Brasil

Abstract

Serological identification of the Grapevine fleck virus in BrazilIn the State of São Paulo, four different isolates of the virus that induces chlorotic translucent vein break in the minor veins of grapevines (Vitis spp.) were separated by their differential biological behaviour and tested for their relationship to Grapevine fleck virus (GFkV) by DAS-ELISA and TAS-ELISA with commercial antisera against GFkV. Young leaves from spring shoots and dormant cuttings were used for antigen extraction. All infected plants were previously indexed by graftinoculation to 'Rupestris du Lot'. The four isolates gave positive reactions to the GFkV antiserum. Positive ELISA reactions were observed in 66 samples of 26 varieties, from 11 grape-growing areas from the State of São Paulo as well as 24 samples, of 12 varieties, from five other states of Brazil. Healthy plants consistently gave negative results. Young leaves were better sources of antigen than cortical scrapings of dormant cuttings. The results showed that the four isolates have a serological relationship with the GFkV, suggesting that they belong to the viral complex that induce grapevine fleck disease.

Prospecção Clonal E Ocorrência De Viroses Da Cultivar Folha De Figo Na Região De Caldas, Mg

“…Segundo Martelli (2006), os vírus considerados mais importantes e mais difundidos da videira são: vírus da canelura do tronco da videira (Grapevine virus A -GVA), vírus do fendilhamento cortical da videira (Grapevine virus B -GVB), vírus do enrolamento das folhas da videira (Grapevine leafroll virus -GFRV), vírus do mosaico do Traviú (Grapevine fanleaf virus -GFLV) e vírus associado ao enrolamento da folha da videira (Grapevine leafroll associated virus -GLRaV-1 e -3). Para se confirmar a presença de vírus em vegetais, recorre-se a testes de diagnose, tais como: sorologia (ELISA), microscopia eletrônica, técnicas moleculares e plantas indicadoras (Kuniyuki et al, 2002).…”