2014
DOI: 10.1590/s0100-40422014000100009
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Floculação de Chlorella sp. produzida em concentrado de dessalinização e estudo de método de extração de lipídeos intracelulares

Abstract: EVALUATION OF FLOCCULATION AND LIPID EXTRACTION OF Chlorella sp. CULTIVATED IN CONCENTRATED DESALINATION.Chlorella sp. was used to assess algal lipid production with concentrated desalination. In order to investigate the action of the flocculating agent calcium chloride and pH, a Box-Behnken Design and a Central Composite Design (CCD) were carried out. Also, Soxhlet and Supercritical Fluid Extraction (SFE), with and without sonication lipid extraction methods, were examined. The optimal flocculation conditions… Show more

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Cited by 16 publications
(6 citation statements)
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“…When the microalgal culture reached a stationary growth phase after 14 days, all of the culture volume from the open raceway pond was pumped into a 4000-L open-top conical tank. Based on previous laboratory results, as reported by Morioka et al (2014) for Chlorella flocculation, the pH of the culture was elevated to 10.5-12.0 by adding (NaOH) + 0.5-1.0 g L -1 of flocculant CaCl 2 . The suspension was manually mixed with a tool (shovel) for 10-15 min and then left to stand overnight (12 h).…”
Section: Ponds Culture Conditions and Growth Measurementsmentioning
confidence: 97%
“…When the microalgal culture reached a stationary growth phase after 14 days, all of the culture volume from the open raceway pond was pumped into a 4000-L open-top conical tank. Based on previous laboratory results, as reported by Morioka et al (2014) for Chlorella flocculation, the pH of the culture was elevated to 10.5-12.0 by adding (NaOH) + 0.5-1.0 g L -1 of flocculant CaCl 2 . The suspension was manually mixed with a tool (shovel) for 10-15 min and then left to stand overnight (12 h).…”
Section: Ponds Culture Conditions and Growth Measurementsmentioning
confidence: 97%
“…Onde, Y é a resposta, β 0 é o intercepto, βi é o coeficiente linear, β ii é o coeficiente quadrático, β ij é o coeficiente da interação, X i e X j são variáveis independentes codificadas e ε corresponde ao resíduo deixado pelo modelo (Morioka et al, 2014).…”
Section: Materiais E Métodosunclassified
“…Estudos preliminares foram realizados para determinar os níveis dos fatores, que estão incluídos na Tabela 1. Onde, Y é a resposta, β 0 é o intercepto, βi é o coeficiente linear, β ii é o coeficiente quadrático, β ij é o coeficiente da interação, X i e X j são variáveis independentes codificadas e ε corresponde ao resíduo deixado pelo modelo (Morioka et al, 2014).…”
Section: Materiais E Métodosunclassified