2013
DOI: 10.1590/s0100-40422013000700020
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Simultaneous and accurate determination of water- and fat-soluble vitamins in multivitamin tablets by using an RP-HPLC method

Abstract: Recebido em 14/10/12; aceito em 22/2/13; publicado na web em 24/5/13In the present study, a reversed-phase high-performance liquid chromatographic (RP-HPLC) procedure was developed and validated for the simultaneous determination of seven water-soluble vitamins (thiamine, riboflavin, niacin, cyanocobalamin, ascorbic acid, folic acid, and p-aminobenzoic acid) and four fat-soluble vitamins (retinol acetate, cholecalciferol, α-tocopherol, and phytonadione) in multivitamin tablets. The linearity of the method was … Show more

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Cited by 21 publications
(12 citation statements)
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References 28 publications
(30 reference statements)
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“…Ultraviolet (UV) absorbance was recorded at 270 nm at room temperature [11,12]. To determine the B group vitamins, the quality assurance (QA) and quality control (QC) for the HPLC were regulated by the method of Kucukkolbasi et al, (2013) with some modifications [13].…”
Section: Determination Of B Group Vitaminsmentioning
confidence: 99%
See 1 more Smart Citation
“…Ultraviolet (UV) absorbance was recorded at 270 nm at room temperature [11,12]. To determine the B group vitamins, the quality assurance (QA) and quality control (QC) for the HPLC were regulated by the method of Kucukkolbasi et al, (2013) with some modifications [13].…”
Section: Determination Of B Group Vitaminsmentioning
confidence: 99%
“…Twenty microliters of extracted oil was directly injected into the HPLC column in triplicate [11,14]. To determine the B group vitamins, the QA and QC for the HPLC were regulated by the method of Kucukkolbasi et al, (2013) with some modifications [13].…”
Section: Determination Of Fat-soluble Vitaminsmentioning
confidence: 99%
“…The obtained run time is considerably shorter in comparison to other similar published HPLC-UV methods. [4][5][6]9,12 …”
Section: Optimization Of Chromatographic Conditionsmentioning
confidence: 99%
“…Due to the large number of commercially available vitamin preparations and their widespread use, the quality control of these preparations is extremely important, especially because of the risk of toxicity from excessive intake of vitamins A and D. 3 HPLC methods, coupled with different detection techniques: UV, [4][5][6][7][8][9][10][11][12] FLD, 6 ECD, 13 and MS 5,14 offer the best approach to accurate content determination of the main FSVs in foods, pharmaceuticals and nutritional supplements. The published HPLC-UV methods for quality control of preparations containing FSVs are quite limited in terms of separation and simultaneous determination of FSVs, 4,5,7,8,11 often have run time longer than 15 min [4][5][6]9,12 and generally require complicated and time-consuming sample preparation. 11,12,15,16 Further on, to our knowledge none of the published HPLC methods offer simultaneous determination of FSVs and also β-carotene and coenzyme Q10, which are often found along in medicines and nutritional supplements.…”
Section: Introductionmentioning
confidence: 99%
“…From the aforesaid methods, RP-HPLC is extensively used instead of other methods because of its higher sensitivity. The existing RP-HPLC methods are for determination of multivitamin in human blood and plasma [6], capsules [7] and tablet dosage forms [8][9][10][11][12][13]. The main advantage of our study is to have a simple, specific, precise, accurate and linear reverse phase gradient HPLC method for qualitative, quantitative and simultaneous determination of vitamin B1, vitamin B2, vitamin B3, vitamin B6 and vitamin C in solid oral powder for veterinary consumption that was not reported before for these five vitamins at the same time.…”
Section: Introductionmentioning
confidence: 99%