Preparative separation and purification of bufadienolides from ChanSu by high-speed counter-current chromatography combined with preparative HPLC

Li, Jialian; Zhang, Yongqing; Yang, Lin; Wang, Xiao; Fang, Liwei; Geng, Yanling; Zhang, Qinde

doi:10.1590/s0100-40422013000500013

Cited by 6 publications

(5 citation statements)

References 10 publications

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“…Three compounds, telocinobufagin, marinobufagin, and bufalin, were found in all samples [ 2 ]. These results have been confirmed by other studies using different analytical methods [ 28 , 29 ]. The indolealkylamines in Chansu have been analyzed in another study, including bufotenine, bufotenidine, bufobutanoic acid, serotonin, bufotenine N -oxide and N -methyl serotonin were also identified [ 30 ].…”

Section: Chemicals Components In Different Species Of Toadssupporting

confidence: 88%

The Development of Toad Toxins as Potential Therapeutic Agents

Zulfiker

et al. 2018

Toxins

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Toxins from toads have long been known to contain rich chemicals with great pharmaceutical potential. Recent studies have shown more than 100 such chemical components, including peptides, steroids, indole alkaloids, bufogargarizanines, organic acids, and others, in the parotoid and skins gland secretions from different species of toads. In traditional Chinese medicine (TCM), processed toad toxins have been used for treating various diseases for hundreds of years. Modern studies, including both experimental and clinical trials, have also revealed the molecular mechanisms that support the development of these components into medicines for the treatment of inflammatory diseases and cancers. More recently, there have been studies that demonstrated the therapeutic potential of toxins from other species of toads, such as Australian cane toads. Previous reviews mostly focused on the pharmaceutical effects of the whole extracts from parotoid glands or skins of toads. However, to fully understand the molecular basis of toad toxins in their use for therapy, a comprehensive understanding of the individual compound contained in toad toxins is necessary; thus, this paper seeks to review the recent studies of some typical compounds frequently identified in toad secretions.

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Section: Chemicals Components In Different Species Of Toadssupporting

confidence: 88%

The Development of Toad Toxins as Potential Therapeutic Agents

Zulfiker

et al. 2018

Toxins

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“…Peaks A ( t R = 9.9 min; λ max = 271 nm), B ( t R = 10.2 min; λ max = 271 nm) and C ( t R = 15.4 min; λ max = 281 nm) were attributed to benzoic acid derivatives, while peak E ( t R = 19.4 min; λ max = 309 nm) to a cinnamic acid derivative. Peaks D ( t R = 16.5 min; λ max = 290 nm) and F ( t R = 19.9 min; λ max = 296 nm) were assigned to bufadienolides …”

Section: Resultsmentioning

confidence: 99%

Anti‐HSV‐1 and HSV‐2 Flavonoids and a New Kaempferol Triglycoside from the Medicinal Plant Kalanchoe daigremontiana

Ürményi

Saraiva

Casanova

et al. 2016

Chemistry & Biodiversity

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Kalanchoe daigremontiana (Crassulaceae) is a medicinal plant native to Madagascar. The aim of this study was to investigate the flavonoid content of an aqueous leaf extract from K. daigremontiana (Kd), and assess its antiherpetic potential. The major flavonoid, kaempferol 3-O-β-d-xylopyranosyl-(1 → 2)-α-l-rhamnopyranoside (1), was isolated from the AcOEt fraction (Kd-AC). The BuOH-soluble fraction afforded quercetin 3-O-β-d-xylopyranosyl-(1 → 2)-α-l-rhamnopyranoside (2) and the new kaempferol 3-O-β-d-xylopyranosyl-(1 → 2)-α-l-rhamnopyranoside-7-O-β-d-glucopyranoside (3), named daigremontrioside. The crude extract, Kd-AC fraction, flavonoids 1 and 2 were evaluated using acyclovir-sensitive strains of HSV-1 and HSV-2. Kd-AC was highly active against HSV-1 (EC = 0.97 μg/ml, SI > 206.1) and HSV-2 (EC = 0.72 μg/ml, SI > 277.7). Flavonoids 1 and 2 showed anti-HSV-1 (EC = 7.4 μg/ml; SI > 27 and EC = 5.8 μg/ml; SI > 8.6, respectively) and anti-HSV-2 (EC = 9.0 μg/ml; SI > 22.2 and EC = 36.2 μg/ml; SI > 5.5, respectively) activities, suggesting the contribution of additional substances to the antiviral activity.

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“…In recent years, natural drug discovery has been intensified and extended to structurally- and functionally-diverse compounds of amphibian origin. In reality, since ancient times, amphibian-derived substances have been applied for many conditions; for instance, the skin secretion of poison-dart frogs have been used for the purpose of effective hunting by Native Americans up to the present [26,27], and Chan Su, processed from the dried skin secretion of Bufo bufo gargarizans Cantor or Bufo melanostictus Schneider with the efficacy of detoxification, has been an important traditional remedy for around 3000 years [28,29,30,31]. With the advancement in the technologies of isolation, identification and analysis, it has been recognised that the skin of amphibians is an extraordinary arsenal of a plethora of biologically-active components possessing diverse pharmacological properties [7,8,9].…”

Section: Discussionmentioning

confidence: 99%

A Hylarana latouchii Skin Secretion-Derived Novel Bombesin-Related Pentadecapeptide (Ranatensin-HLa) Evoke Myotropic Effects on the in vitro Rat Smooth Muscles

Lin

et al. 2019

Toxins

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Amphibians have developed successful defensive strategies for combating predators and invasive microorganisms encountered in their broad range of environments, which involve secretion of complex cocktails of noxious, toxic and diverse bioactive molecules from the skins. In recent years, amphibian skin secretions have been considered as an extraordinary warehouse for the discovery of therapeutic medicines. In this study, through bioactivity screening of the Hylarana latouchii skin secretion-derived fractions, a novel peptide belonging to ranatensin subfamily (ranatensin-HLa) was discovered, and structurally and pharmacologically-characterised. It consists of 15 amino acid residues, pGlu-NGDRAPQWAVGHFM-NH2, and its synthetic replicate was found to exhibit pharmacological activities on increasing the contraction of the in vitro rat bladder and uterus smooth muscles. Corresponding characteristic sigmoidal dose-response curves with EC50 values of 7.1 nM and 5.5 nM were produced, respectively, in bladder and uterus. Moreover, the precursor of ranatensin-HLa showed a high degree of similarity to those of bombesin-like peptides from Odorrana grahami and Odorrana schmackeri. Hylarana latouchii skin continues to serve as a storehouse with diverse lead compounds for the development of therapeutically effective medicines.

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Preparative separation and purification of bufadienolides from ChanSu by high-speed counter-current chromatography combined with preparative HPLC

Cited by 6 publications

References 10 publications

The Development of Toad Toxins as Potential Therapeutic Agents

The Development of Toad Toxins as Potential Therapeutic Agents

Anti‐HSV‐1 and HSV‐2 Flavonoids and a New Kaempferol Triglycoside from the Medicinal Plant Kalanchoe daigremontiana

A Hylarana latouchii Skin Secretion-Derived Novel Bombesin-Related Pentadecapeptide (Ranatensin-HLa) Evoke Myotropic Effects on the in vitro Rat Smooth Muscles

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