Quantificação dos isômeros ácido L-ascórbico e ácido D-iso-ascórbico em geleias de frutas por cromatografia líquida de alta eficiência

Oliveira, Raquel Grando de; Godoy, Helena Teixeira; Prado, Marcelo Alexandre

doi:10.1590/s0100-40422012000500028

Cited by 5 publications

(1 citation statement)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The values obtained for the peaks from the calibration curves for [Cr(III)-EDTA] − and Cr(VI) were 1.2 and 1.4, respectively. The values can be considered satisfactory as they are below 1.5 and indicate low tail influence on the peaks [ 38 ].…”

Section: Resultsmentioning

confidence: 99%

Chromium Speciation by HPLC-DAD/ICP-MS: Simultaneous Hyphenation of Analytical Techniques for Studies of Biomolecules

Procópio

Pereira

Lange

et al. 2023

IJERPH

View full text Add to dashboard Cite

The first element legislated adopting chemical speciation was chromium (Cr) for differentiation between the highly toxic Cr(VI) from the micronutrient Cr(III). Therefore, this work aimed to develop a new analytical method through the coupling of High-Performance Liquid Chromatography with Diode-Array Detection (HPLC-DAD) with inductively coupled plasma mass spectrometry (ICP-MS) to obtain molecular and elemental information simultaneously from a single sample injection. In the first step, a low-cost flow split made of acrylic was developed aiming at optimally directing the sample to the detectors, enabling the HPLC-DAD/ICP-MS coupling. After the extraction of Certified Reference Materials (CRM of natural water NIST1640a and sugar cane leaf agro FC_012017), the recoveries determined by ICP-MS were 99.7% and 85.4%, respectively. Then, the method of HPLC-DAD/ICP-MS was applied for real samples of the CRMs. The presence of possible biomolecules associated with Cr(III) and Cr(VI) species was evaluated, with the simultaneous response detection of molecular (DAD) and elementary (ICP-MS) detectors. Potential biomolecules were observed during the monitoring of Cr(VI) and Cr(III) in sugar cane leaves, water samples and a supplement of Cr picolinate. Finally, the article also discusses the potential of the technique applied to biomolecules containing other associated elements and the need of more bioanalytical methods to understand the presence of trace elements in biomolecules.

show abstract

Section: Resultsmentioning

confidence: 99%