Desenvolvimento e validação de método analítico por CLAE para a quantificação simultânea de dipropionato de betametasona e fosfato sódico de betametasona em suspensão injetável

Simon, Alice; Cabral, Lúcio Mendes; Sousa, Valéria Pereira de

doi:10.1590/s0100-40422012000300027

Cited by 5 publications

(3 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Considering retention times for ACV (2.93 min) and CUR (11.57 min), and peaks resolution, it was choose as the most suitable mobile phase the mixture ACN:H 3 PO 4 0.1%:MeOH (50:40:10). Although these chromatographic conditions have not demonstrated suitable results for all of the parameters (Table 1), the obtained results were very similar to those in a previous study about simultaneous quantification (Simon, 2012;Moorthi, 2013b;Moorthi, 2013a). The PDA detectors allow analysis of one or more substances at different wavelengths, as well as verification of the chromatographic peak purity.…”

Section: Resultssupporting

confidence: 81%

Development and validation of high-performance liquid chromatography method for simultaneous determination of acyclovir and curcumin in polymeric microparticles

2018

J App Pharm Sci

View full text Add to dashboard Cite

This study aimed to develop and validate a HPLC based analytical methodology for simultaneous determination of acyclovir and curcumin within microparticles. Chromatographic separation was achieved by employing a Fenomenex C18 column as stationary phase and a ternary mixture of acetonitrile, 0.1% phosphoric acid, and methanol (50:40:10) as the mobile phase. The validated method proved to be linear in the range of 0.5-30 µg.mL −1 and 0.5-20 µg.mL −1 for acyclovir and curcumin, respectively. Detection and quantification limits for acyclovir were, respectively, 83.62 ng.mL −1 and 109.52 ng.mL −1 , while for curcumin the values were 91.61 ng.mL −1 and 128.71 ng.mL −1 , what assures the methodology sensitivity. The method was also precise (1.2% RSD for acyclovir and 1.38% RSD for curcumin), besides showing recovery rates close to 100% for both of two drugs when accuracy was accessed. Minor alterations over chromatographic setup have confirmed methodology robustness. The present methodology proved to be capable of detecting and quantifying acyclovir and curcumin at polymeric microparticles in a single run, showing itself as an analytical alternative to be employed in the quality control for this dosage form.

show abstract

Section: Resultssupporting

confidence: 81%

Development and validation of high-performance liquid chromatography method for simultaneous determination of acyclovir and curcumin in polymeric microparticles

2018

J App Pharm Sci

View full text Add to dashboard Cite

show abstract

“…The equation of a typical calibration curve for AS with SC homogenate was y = 9.8840 x + 20.7214 (r = 0.9992) and for RS was y = 4.2943 x − 5.8857 (r = 0.9981). The validity of the regression and linear model of the calibration curve was assessed by analysis of variance by F-test, comparing the F calc (calculated F) and F tab (tabulated F) values [25]. When the slope of the curve is significantly different from zero, the regression is valid.…”

Section: Resultsmentioning

confidence: 99%

Development of a High-Performance Liquid Chromatographic Method for Asiaticoside Quantification in Different Skin Layers after Topical Application of a Centella asiatica Extract

et al. 2017

View full text Add to dashboard Cite

The topical application of extract has been commonly used for many different purposes but especially for cosmetic use in the treatment of gynoid lipodystrophy. Asiaticoside, the most active component in this extract, is responsible for its therapeutic activities. However, little is known to date about asiaticoside skin penetration. Thus, an analytical method for asiaticoside quantification in different skin layers after the topical application of extract was developed and skin permeation studies were performed with the plant extract to apply the analytical method developed. An extraction procedure to recover asiaticoside from the biological matrix was also developed. Asiaticoside was assayed by HPLC/UV (high-performance liquid chromatography-ultraviolet detection) using a gradient of ACN (acetonitrile) and 0.2% phosphoric acid (flow rate of 1.0 mL/min). The analytical procedure was validated according to U. S. Food and Drug Administration guidelines. Selectivity was shown, as endogenous skin components did not interfere with the asiaticoside peak. Analytical curve was linear (3 to 60 µg/mL) and the lower limit of quantification was determined (3 µg/mL). Asiaticoside recoveries from skin samples were 95.1% and 66.7% for the stratum corneum and remaining skin, respectively. After 48 h of permeation studies, a substantial amount of asiaticoside was quantified in the skin layers. The presence of asiaticoside was also detected in the receptor solution of Franz diffusion cells after 48 h (5.81 ± 1.00 µg/mL). The method was reliable and reproducible for asiaticoside quantification in skin samples, thereby making it possible to determine the cutaneous penetration profile of this drug in permeation studies.

show abstract

“…The method used was based on the chromatographic conditions developed by our research group for the simultaneous quantification of BD and BSP in injectable suspensions (18). The stationary phase was the column C18 Kromasil 100-5 (4.6×150 mm, 5 mm), and the mobile phase was a ternary gradient, containing phosphate buffer (pH 4.5; 0.07 M), acetonitrile, and methanol, at a flow rate of 1.6 mL/ min with a running time of 17 min (18).…”

Section: Bsp and Bd Quantification In Dissolution Testsmentioning

confidence: 99%

Development and Validation of a Discriminative Dissolution Test for Betamethasone Sodium Phosphate and Betamethasone Dipropionate Intramuscular Injectable Suspension

et al. 2013

Self Cite

View full text Add to dashboard Cite

Abstract. The intramuscular administration of the injectable suspension betamethasone sodium phosphate (BSP) and betamethasone dipropionate (BD) has immediate therapeutic activity due to solubilized BSP and prolonged activity resulting from the slow release of BD micro-crystals. The purpose of this study was to develop and validate a dissolution method for BD in intramuscular injectable suspensions with detection by high-performance liquid chromatography (HPLC) method. Five commercial products presented a distribution of particle sizes, ranging between 7.43 and 40.25 μm as measured by laser diffraction. It was also found that particle sizes differed between batches of the same product. The different products were tested using the paddle apparatus, with stirring speeds of 25 and 50 rpm in 300 mL of phosphate buffer; simulated body fluid, muscle fluid, and synovial fluid were used as biorelevant dissolution media at 37±0.5°C. It was verified that not only does average particle size affect the dissolution rate, but also the mode and the polydispersity index of the particles. Discriminatory power was obtained using the in vitro dissolution method with 0.1 M sodium phosphate buffer pH 7.4 containing 0.1% sodium lauryl sulfate and a stirring speed of 50 rpm. The HPLC-method is linear, precise, selective, and accurate for the quantification of BSP and BD in dissolution profile testing. This dissolution method can be utilized as a method to control the quality of these injectable suspensions.KEY WORDS: dipropionate betamethasone; dissolution test; intramuscular injectable suspensions; simulated muscular fluid; sodium phosphate betamethasone.

show abstract

Desenvolvimento e validação de método analítico por CLAE para a quantificação simultânea de dipropionato de betametasona e fosfato sódico de betametasona em suspensão injetável

Cited by 5 publications

References 7 publications

Development and validation of high-performance liquid chromatography method for simultaneous determination of acyclovir and curcumin in polymeric microparticles

Development and validation of high-performance liquid chromatography method for simultaneous determination of acyclovir and curcumin in polymeric microparticles

Development of a High-Performance Liquid Chromatographic Method for Asiaticoside Quantification in Different Skin Layers after Topical Application of a Centella asiatica Extract

Development and Validation of a Discriminative Dissolution Test for Betamethasone Sodium Phosphate and Betamethasone Dipropionate Intramuscular Injectable Suspension

Contact Info

Product

Resources

About