2010
DOI: 10.1590/s0100-40422010000900012
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Purificação e caracterização da quitinase de uva (Vitis vinífera L. cv Red Globe) para a produção de quitosana a partir de quitina de camarão

Abstract: Chitinase is produced by a wide variety of plants as a defense against peste attacks. In this study, grape chitinases were purified 16 times by fractionation in 80% ammonium sulfate followed by dialysis and filtration. Purified chitinases exhibited enzymatic activity toward chitin azure. The yield of purified chitinase was 229 mg/L with chitinase activity of 563 U/g. Chitinases had molecular masses of 24 and 30 kDa, as evaluated by SDS-PAGE 12.5%. Two pH optima were determined 3.0 and 6.0. The optimal temperat… Show more

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Cited by 8 publications
(4 citation statements)
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“…When plants are attacked, metabolic processes that induce ethylene and salicylic acid production are activated which, in turn, stimulates chitinases production and increases in immature fruit concentrations. These enzymes can correspond to 50% of the soluble protein concentration found in fruits under these conditions (Patel et al 2016, Gomes et al 2010.…”
Section: Grapes (Vitis Vinifera L and Vitis Lobruscas L)mentioning
confidence: 99%
“…When plants are attacked, metabolic processes that induce ethylene and salicylic acid production are activated which, in turn, stimulates chitinases production and increases in immature fruit concentrations. These enzymes can correspond to 50% of the soluble protein concentration found in fruits under these conditions (Patel et al 2016, Gomes et al 2010.…”
Section: Grapes (Vitis Vinifera L and Vitis Lobruscas L)mentioning
confidence: 99%
“…26,27 Cellulases, pectinases, pepsins, papai s, eutral proteases, lipases a d αamylases show the ability to hydrolyze CS at comparable activity levels, but with different specificities. [28][29][30] Our research group focused on the production of CS by a binary enzyme system, comprised by a hydrolytic step catalyzed by chitinase purified from grapes 31 , followed by a subsequent deacetylation, where the removal of the acetate radical from the chitin D-glucosamine monomers is catalyzed by a recombinant chitin deacetylase enzyme from aker's yeast ut lo ed i Pichia pastoris. 32 The aforementioned enzymes can be obtained from different organisms and their use for chitin hydrolysis has been explored in order to improve the process efficiency and control over the physicochemical characteristics of the formed products.…”
Section: Cs Manufacturing: Enzymatic Vs Chemical Processmentioning
confidence: 99%
“…Chitinases were obtained from Vitis vinifera L. cv "Red Globe" and purified by fractionation in 80% (v/v) ammonium sulfate, followed by dialysis and filtration [21].…”
Section: Preparation Of Chitinasesmentioning
confidence: 99%
“…Biocatalytic conversion of chitosans using the purified forms of both chitinases and chitin deacetylase has been described in few studies [21]. Recently, several comercially available hydrolytic enzymes including lysozyme, cellulase, papain, pectinases, and hemicellulase were found to catalyze the cleavage of the glycoside bond in chitosan [26,41].…”
Section: Substratementioning
confidence: 99%