Determinação de hormônios estrógenos em água potável usando CLAE-DAD

Verbinnen, Raphael Teixeira; Nunes, Gilvanda Silva; Vieira, Eny María

doi:10.1590/s0100-40422010000900003

Cited by 23 publications

(7 citation statements)

References 17 publications

(30 reference statements)

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“…The E 1 , E 2 and E 3 are natural compounds derived from the metabolism of man and mammals in general. On the other hand, EE 2 is a synthetic compound, being widely used for making contraceptive pills .…”

Section: Introductionmentioning

confidence: 99%

Total Determination of Estrogenic Phenolic Compounds in River Water Using a Sensor Based on Reduced Graphene Oxide and Molecularly Imprinted Polymer

2018

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The contamination of water and wastewater by emerging pollutants, due to the anthropogenic activities, are an environmental problem that generates several negative impacts. In this range of species, steroids have gaining notoriety because their action of endocrine‐disruption. In this work, they are called estrogenic phenolic compounds (EPCs): estrone (E1), estradiol (E2), ethinyl estradiol (EE2) and estriol (E3), for determination using an electrochemical sensor based on reduced graphene oxide (rGO) and molecularly imprinted polymer (MIP). The analytical method developed, allied with the experimental and operational optimizations, proved to be effective for the total quantification of the EPCs in river water. The method shows sensitivity of 1.12 μA/μmol L−1, detection limit of 26.8 nmol L−1 and linear range of 0.16–15 μmol L−1. The similar electrochemical behavior of the four compounds studied (E1, E2, EE2 and E3) and the efficiency of the modified composite (rGO, MIP) in the fabrication of the sensor resulted in high electrical conductivity and selective adsorptivity, respectively.

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Section: Introductionmentioning

confidence: 99%

Total Determination of Estrogenic Phenolic Compounds in River Water Using a Sensor Based on Reduced Graphene Oxide and Molecularly Imprinted Polymer

2018

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“…Chronic exposure of adult males to estrogenic substances may result in breast growth and interference with the hypothalamus, pituitary and gonadal glandular systems, resulting in decreased libido, impotence, decreased androgen levels in the blood and decreased sperm count (Auger, Kunstmann, Czyglik, & Jouannet, 1995;Waring & Harris, 2005). Furthermore, contaminant estrogens, with relevance to EE2, are thought to induce some diseases, such as uterus, breast and prostate cancer, male fertility reduction, abnormal sexual development, abnormal thyroid glands, increased polycystic ovaries incidence, disturbances in ovarian functions, fertilization and pregnancy, endometriosis and neurobehavioral effects (Soto, Justicia, Wray, & Sonnenschein, 1991;Adeel, Song, Wang, Francis, & Yang, 2017;Verbinnen, Nunes, & Vieira, 2010;Birnbaum & Fenton, 2003;Coleman, Abdullah, Eggins, & Palmer, 2005;Solomon & Schettler, 2000;Gray, 1998;Daston et al, 1997). Finally, Sodr e, Locatelli, Montagner, and Jardim (2007) emphasize that the embryonic stage is much more susceptible to estrogens compared to adults.…”

Section: Steroid Estrogensmentioning

confidence: 99%

Environmental impact and biological removal processes of pharmaceutically active compounds: The particular case of sulfonamides, anticonvulsants and steroid estrogens

Leal

Mesquita

Amaral

et al. 2019

Critical Reviews in Environmental Science and Technology

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Pharmaceutically active compounds (PhACs) have recently received wide attention in the scientific community due to their extensive consumption for human health and consequent discharge to the environment. Release of PhACs into the environment, even in trace amounts, can cause serious environmental damage. This has become a major concern and their removal from water sources is a priority. Although a few PhACs are efficiently removed in wastewater treatment plants (WWTPs), others remain recalcitrant, and their release is causing damage. In this review, the current state of the art on the biological removal processes of sulfonamide sulfamethoxazole (SMX), the anticonvulsant carbamazepine (CBZ), and steroid estrogens 17b-estradiol (E2) and 17a-ethinylestradiol (EE2) are discussed, along with their environmental impact. Other systems beyond activated sludge, such as membrane bioreactors, enzymatic membrane reactors, fungi treatments and hybrid systems are also becoming of major interest and are being evaluated for the removal of these compounds. Future perspectives are addressed.

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“…Despite that, these instruments are expensive and require high investment costs for their maintenance. 29 High-performance liquid chromatography coupled with UV (HPLC-UV) [30][31][32] and fluorescence detection (HPLC-FLD) 26,29,[33][34][35] have been reported as low-cost alternatives to HPLC-MS analysis, used to determine estrogens with quantification limits compatible with the concentration of estrogens in wastewater and natural waters. Lopes et al 36 showed the determination of E1 and E2 by SPE-HPLC-FLD in natural (600 ng L -1 of E1 in surface water, 6 ng L -1 of E2 in river spring) and treated waters (6.8 ng L -1 of E2) in São Paulo (Brazil).…”

Section: Introductionmentioning

confidence: 99%

Sensitive Estrogens Determination in Wastewater Samples by HPLC and Fluorescence Detection

Liz¹,

Amaral²,

Stets³

et al. 2016

J. Braz. Chem. Soc.

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A sensitive high-performance liquid chromatography-fluorescence detection method (HPLC-FLD) was developed and validated for 17β-estradiol (E2) and 17α-ethinylestradiol (EE2) determination in wastewater samples. Sample preparation was performed by solid-phase extraction (SPE) on Hypersep C18 cartridges. Conditioning and elution solvents and sample volume were investigated by a 2 3 factorial design for both analytes. The pre-concentration factor was 2500 times, allowing to quantify 5.0 ng L -1 for both hormones. The SPE-HPLC-FLD method was successfully applied to detect and quantify trace amount of target compounds in raw and treated wastewater samples. E2 was found below limit of quantification in influent and effluent samples. Nevertheless, the recoveries of E2 in standard addition experiments were between 41 and 72%, with relative standard deviations between 4 and 16%. An important matrix effect was observed in the determination of EE2, with fluorescence signal suppression. Keywords: estrogens, SPE, HPLC, fluorescence, wastewater IntroductionContamination of water resources represents a worldwide concern. Excessive population growth with the inefficient wastewater collection, particularly in developing countries, low treatment efficiency and inadequate sewage disposal, are the common causes of water contamination.1 Endocrine disrupting compounds (EDCs), especially estrogens, are chemical species that have negative activity on the endocrine system in humans 2,3 and animals. 4,5 Estrone (E1), 17β-estradiol (E2) and estriol (E3) are estrogens naturally produced by mammals and excreted mainly by pregnant and menstrual women via urine and feces.1 Otherwise, 17α-ethinylestradiol (EE2) is a synthetic estrogen widely used as contraceptive and for therapeutic purposes such menopausal syndrome and prostate cancer control. 6 Despite of being unregulated contaminants in environmental waters, they have already been included in the New Contaminant Candidate List-3 of the U.S.EPA and in the list of priority substances of the EU Water Framework Directive. 7,8 These compounds have been detected in wastewater treatment plants (WWTP), [9][10][11][12] in natural waters 9,13 and, even in water supplies.14 Since 1990, estrogenic activity has been detected in many countries. For instance, E1, E2, and EE2 were found in concentrations about ng L -1 in the effluent from a sewage treatment plant in the UK. 15 In Brazil, monitoring studies of natural estrogens in domestic sewage also were initiated in the 90s. 16 This study revealed estrogen concentrations between 20 and 40 ng L -1 and only partial removal by conventional treatments based on biological filter and activated sludge systems. Since then, other monitoring works reported the presence of endocrine disruptors in surface waters in Brazil. Sodré et al. 17 The estrogens monitoring in sewage and natural waters is a challenge due to the low concentration of the analytes and the complexity of the environmental matrices. Most of the reported methods to estrogens determination in aque...

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Determinação de hormônios estrógenos em água potável usando CLAE-DAD

Cited by 23 publications

References 17 publications

Total Determination of Estrogenic Phenolic Compounds in River Water Using a Sensor Based on Reduced Graphene Oxide and Molecularly Imprinted Polymer

Total Determination of Estrogenic Phenolic Compounds in River Water Using a Sensor Based on Reduced Graphene Oxide and Molecularly Imprinted Polymer

Environmental impact and biological removal processes of pharmaceutically active compounds: The particular case of sulfonamides, anticonvulsants and steroid estrogens

Sensitive Estrogens Determination in Wastewater Samples by HPLC and Fluorescence Detection

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