Determination of rutin and narcissin in marigold extract and topical formulations by liquid chromatography: applicability in skin penetration studies

Fonseca, Yris Maria; Vicentini, Fabiana T. M. C.; Catini, Carolina Dias; Fonseca, Maria José Vieira

doi:10.1590/s0100-40422010000600019

Cited by 11 publications

(5 citation statements)

References 17 publications

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“…In the literature, the antioxidant activity of the aqueous and alcoholic extracts of pot marigold flowers obtained by maceration was reported. In this paper, the IC 50 value for the extract obtained using the UAE was almost the same as in the case of maceration with 50% (v/v) ethanol at 25°C for 5 days (IC 50 = 97.1 µg/ml)(Fonseca et al, 2010) García-Risco et al (2017). concluded that the strongest antioxidant activity (EC 50 = 123.35 mg/ml) expressed the 50% (v/v) ethanolic extract obtained using the UAE.…”

supporting

confidence: 62%

Food additive based on the encapsulated pot marigold ( Calendula officinalis L.) flowers extract in calcium alginate microparticles

Gajić

Savić

Skrba

et al. 2021

Food Processing Preservation

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The aim of this paper was to prepare a natural food additive based on the encapsulated aqueous extract of pot marigold flowers in the calcium alginate microparticles. The prepared extract had an antioxidants content of 5.59 g GAE/100 g of dry weight. Its antioxidant activity was expressed based on a half‐maximal inhibitory concentration which was found to be 0.096 mg/ml. The average particle sizes of control and encapsulated alginate microparticles prepared using extrusion‐assisted by a secondary airflow were 643.7 and 789.3 µm, respectively. The encapsulation efficiency of 87.4% was determined. The study has shown that the chemical interactions between bioactive compounds and alginate did not occur. The release of antioxidants from alginate microparticles was monitored in the conditions of the simulated gastrointestinal tract at 37°C. The higher release of antioxidants by about 70% was noticed in the simulated intestinal fluid. The application of alginate microparticles can ensure the safety of foodstuffs and increase their nutritional value in a way acceptable to the final consumers. Novelty Impact Statement Studies on the encapsulation of pot marigold flowers into the calcium alginate microparticles using extrusion‐assisted by a secondary airflow have important research value. The prepared system can be used as an additive of foodstuffs for the improvement of nutritional value and enhancement of their stability. The prolonged delivery system can enable the safe storage of products based on the long‐lasting release of antioxidants.

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supporting

confidence: 62%

Food additive based on the encapsulated pot marigold ( Calendula officinalis L.) flowers extract in calcium alginate microparticles

Gajić

Savić

Skrba

et al. 2021

Food Processing Preservation

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“…extract in skin layers, 29 our method was more efficient in the recovery of SN and SM. Furthermore, comparing with the results obtained for SN and SM recoveries from plant biomass by Tiossi et al, 3 it is clear that the recoveries were similar regardless of the matrices and extraction procedures.…”

Section: Figure 2 Hplc-uv Chromatographic Profiles Of Standards Of Smentioning

confidence: 85%

A validated HPLC analytical method for the analysis of solasonine and solamargine in in vitro skin penetration studies

Tiossi¹,

Costa²,

Miranda³

et al. 2012

Quím. Nova

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Recebido em 30/5/12; aceito em 25/8/12; publicado na web em 9/11/12To assess topical delivery studies of glycoalkaloids, an analytical method by HPLC-UV was developed and validated for the determination of solasonine (SN) and solamargine (SM) in different skin layers, as well as in a topical formulation. The method was linear within the ranges 0.86 to 990.00 µg/mL for SN and 1.74 to 1000.00 µg/mL for SM (r = 0.9996). Moreover, the recoveries for both glycoalkaloids were higher than 88.94 and 93.23% from skin samples and topical formulation, respectively. The method developed is reliable and suitable for topical delivery skin studies and for determining the content of SN and SM in topical formulations.

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“…To separate target compounds, only RP C18 columns with varying lengths were used, such as 75 mm ProntoSIL-120-5-C18 [ 90 , 114 , 144 ]; 100 mm Phenomenex C18 [ 142 ], Zorbax SB-C18 [ 143 ], and LiChrosorb RP18 [ 100 , 153 ]; 150 mm Luna C18 [ 131 ], SiliaChrom C-18 [ 140 ], Eclipse XDB-C18 [ 146 ], Spherisorb S3 ODS-2 C18 [ 149 ], Zorbax Eclipse Plus C18 [ 151 ], and Aquity UPLC HSS T3 [ 154 ]; 220 mm Aquapore RP-300 [ 152 ]; 250 mm Shim-pack C-18 [ 96 ], Hypersil C18 [ 141 , 147 ], X-Bridge C18 [ 145 ], and Phenomenex C18 [ 148 ]; 300 mm Bondclone C18 [ 133 ]; and 1000 mm Hypersil Gold [ 75 ]. The presence of various eluents requires the frequent use of formic acid [ 75 , 96 , 142 , 150 , 151 ], acetic acid [ 133 , 141 , 148 ], phosphoric acid [ 140 , 146 ] as the polar eluent and methanol [ 133 , 140 ] and acetonitrile [ 96 , 114 , 141 , 142 ] as the non-polar eluent. The addition of lithium perchlorate [ 90 , 114 , 144 ] and tetrahydrofuran [ 152 ] resulted in better resolution and improved peak shapes.…”

Section: Separation Of Calendula Metabolites By Gc...mentioning

confidence: 99%

Marigold Metabolites: Diversity and Separation Methods of Calendula Genus Phytochemicals from 1891 to 2022

Оленников

Кащенко

2022

Molecules

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Marigold (Calendula), an important asteraceous genus, has a history of many centuries of therapeutic use in traditional and officinal medicines all over the world. The scientific study of Calendula metabolites was initiated at the end of the 18th century and has been successfully performed for more than a century. The result is an investigation of five species (i.e., C. officinalis, C. arvensis, C. suffruticosa, C. stellata, and C. tripterocarpa) and the discovery of 656 metabolites (i.e., mono-, sesqui-, di-, and triterpenes, phenols, coumarins, hydroxycinnamates, flavonoids, fatty acids, carbohydrates, etc.), which are discussed in this review. The identified compounds were analyzed by various separation techniques as gas chromatography and liquid chromatography which are summarized here. Thus, the genus Calendula is still a high-demand plant-based medicine and a valuable bioactive agent, and research on it will continue for a long time.

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Determination of rutin and narcissin in marigold extract and topical formulations by liquid chromatography: applicability in skin penetration studies

Cited by 11 publications

References 17 publications

Food additive based on the encapsulated pot marigold ( Calendula officinalis L.) flowers extract in calcium alginate microparticles

Food additive based on the encapsulated pot marigold ( Calendula officinalis L.) flowers extract in calcium alginate microparticles

A validated HPLC analytical method for the analysis of solasonine and solamargine in in vitro skin penetration studies

Marigold Metabolites: Diversity and Separation Methods of Calendula Genus Phytochemicals from 1891 to 2022

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