Development and validation of UV spectrophotometric method for determination of levofloxacin in pharmaceutical dosage forms

Kassab, Nájla Mohamad; Amaral, Marcos Serrou do; Singh, Anil Kumar; Santoro, María Inés Rocha Miritello

doi:10.1590/s0100-40422010000400037

Cited by 25 publications

(10 citation statements)

References 9 publications

(20 reference statements)

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“…Levofloksasin adalah enantiomer (-)-(S)-murni dari bentuk rasemat obat ofloksasin yang diperkenalkan pada tahun 1997 [2]. Levofloksasin merupakan generasi ketiga dari golongan kuinolon baru dengan penambahan atom fluor pada cincin kuinolon.…”

Section: Pendahuluanunclassified

Optimasi Tablet Levofloksasin yang Mengandung Bahan Pengikat PVP K-30 dan Disintegran Vivasol

2017

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ABSTRACT:The aim of this research was to get optimal formula of levofloxacin tablet prepared with variation of PVP K-30 as binder and vivasol as disintegrant. The making of levofloxacin tablets was done by wet granulation. Tablet was prepared with various levels of PVP K-30 and disintegrant vivasol, compressed using a hydraulic press with 12 mm punch diameter, for 3 seconds. Physical quality (hardness, friability, and disintegration time) and dissolution rate of tablet was evaluated. The optimization of the formula was done by factorial design of 22 factorial experiments with 2 factors (PVP K-30 and vivasol) and 2 levels (2% and 4%). Optimization results showed that elevated levels of PVP K-30 increased tablet hardness, reduced friability of tablet, decreased disintegrating time, and increased dissolution rate of levofloxacin tablets. Meanwhile, elevated levels of vivasol increased the hardness of tablets, decreased the disintegrating time of tablets, decreased the dissolution rate of levofloxacin tablets, but did not affect the friability of tablets. In conclusion, the optimal tablet that meet the specifications of physical quality (hardness, friability, and disintegrating time) and dissolution rate was made by 2.4 to 3.7% of PVP K-30 and 2.0 to 3.2% vivasol as shown in the feasible area of design space. ABSTRAK:Penelitian ini bertujuan mendapatkan formula yang optimal tablet levofloksasin yang dibuat dengan variasi PVP K-30 sebagai pengikat dan vivasol sebagai disintegran. Metode pembuatan tablet levofloksasin dilakukan secara granulasi basah. Formula tablet dibuat dengan variasi PVP K-30 dan disintegran vivasol, dikempa menggunakan alat hidrolik press dengan puch diameter 12 mm, selama 3 detik. Evaluasi mutu fisik (kekerasan, kerapuhan, dan waktu hancur) dan laju disolusi tablet. Optimasi formula dilakukan dengan desain faktorial 22 yaitu eksperimen faktorial dengan 2 faktor (PVP K-30 dan vivasol) dan 2 level (2% dan 4%). Hasil optimasi menunjukkan bahwa peningkatan kadar PVP K-30 meningkatkan kekerasan tablet, menurunkan kerapuhan tablet, dan menurunkan waktu hancur tablet, serta meningkatkan laju disolusi dari tablet levofloksasin. Peningkatan kadar vivasol meningkatkan kekerasan tablet, tidak mempengaruhi kerapuhan tablet, dan menurunkan waktu hancur tablet, serta menurunkan laju disolusi tablet levofloksasin. Kesimpulan penelitian ini menunjukkan bahwa tablet yang optimal serta memenuhi spesifikasi dari mutu fisik (kekerasan, kerapuhan, dan waktu hancur) dan laju disolusi ditunjukkan pada daerah feasible dari design space yaitu kadar PVP K-30 antara 2,4 sampai 3,7 % dan kadar vivasol 2,0 sampai 3,2%.

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Section: Pendahuluanunclassified

Optimasi Tablet Levofloksasin yang Mengandung Bahan Pengikat PVP K-30 dan Disintegran Vivasol

2017

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“…Literature survey revealed that few analytical methods are available for the individual estimation of levofloxacin by HPTLC [11][12] , by HPLC [13][14] and by conductometry 15 . Recently some UV spectrophotometric methods were reported for the estimation of levofloxacin using various solvent such as 0.1M HCl 16 , 100% methanol 17 , acetonitrile 18 and the mixer of water, methanol and acetonitrile 19 . But single estimation of this drug with 0.1M sodium hydroxide as solvent has not been reported in bulk and in pharmaceutical formulation.…”

Section: Quick Response Codementioning

confidence: 99%

Untitled

2014

IJPSR

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The present study was undertaken to develop and validate a simple, accurate, precise, reproducible and cost effective UV-Visible spectrophotometric method for the estimation of levofloxacin in bulk and pharmaceutical formulation. The solvent used throughout the experiment was 0.1N NaOH. Absorption maximum (λ max) of the drug was found to be 288 nm. The quantitative determination of the drug was carried out at 288 nm and Beer's law was obeyed in the range of 2-12μg/mL. The method was shown linear in the mentioned concentrations having line equation y = 0.0704x + 0.0034 with correlation coefficient R 2 of 0.999. The recovery values for levofloxacin ranged from 99.92%-100.33%. The relative standard deviation of six replicates of assay was less than 2%.The percent relative standard deviation (RSD %) of interday precision range was 0.469-0.925 % and intraday precision range was 0.685-0.713%. The limit of detection and limit of quantification was 0.087μg/mL and 0.164μg/mL. The percent relative standard deviation of robustness and ruggedness of the method was 0.136-0.213%. Hence, proposed method was precise, accurate and cost effective. This method could be applicable for quantitative determination of the bulk drug as well as dosage formulation.

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“…Up until now, many analytical techniques have been used for the detection of FQs. For instance, high performance liquid chromatography [22,23], capillary electrophoresis [24,25], liquid chromatography tandem mass spectrometry [26,27] and ultraviolet spectrophotometry [28,29]. Nevertheless, most of the above methods tend to require expensive and sophisticated instruments and complicated pretreatment processes.…”

Section: Introductionmentioning

confidence: 99%

Silver nanoparticles enhanced fluorescence for sensitive determination of fluoroquinolones in water solutions

Wang

et al. 2019

Open Chemistry

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A new type of silver nanoparticle (AgNPs) was prepared with simple and fast methods and low-toxic compounds. With the addition of different concentrations of AgNPs, the effects of AgNPs on the fluorescence properties of three different kinds of fluoroquinolones (enrofloxacin ENR, lomefloxacin LMF and norfloxacin NOR) in water solutions were studied, respectively. The experimental results demonstrated that the fluorescence intensity for each of the fluoroquinolones (FQ)was firstly enhanced and then quenched with the increased concentration of AgNPs in water solutions. The possible mechanisms about the AgNPs on the fluorescence behaviors of each FQ were also investigated, respectively. In addition, new silver enhanced nanoparticles materials fluorescence methods were established for the separate determination of ENR, LMF and NOR in water solutions. As compared with the identical control fluorimetric methods with no addition of AgNPs, the new enhanced fluorimetic methods were also investigated, respectively. The experimental results indicated that the new enhanced methods could detect lower concentrations of ENR, LMF and NOR in water solutions. Moreover, the newly enhanced fluorimetric methods were validated and successfully applied for the quantitative assay of ENR, LMF and NOR in different kinds of medicinal preparations, respectively.

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Development and validation of UV spectrophotometric method for determination of levofloxacin in pharmaceutical dosage forms

Cited by 25 publications

References 9 publications

Optimasi Tablet Levofloksasin yang Mengandung Bahan Pengikat PVP K-30 dan Disintegran Vivasol

Optimasi Tablet Levofloksasin yang Mengandung Bahan Pengikat PVP K-30 dan Disintegran Vivasol

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Silver nanoparticles enhanced fluorescence for sensitive determination of fluoroquinolones in water solutions

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