Development of a simple, rapid and validated spectrophotometric method for nitazoxanide in pharmaceutical formulations and comparison with HPLC

Abstract

Recebido em 2/5/09; aceito em 25/9/09; publicado na web em 25/2/10 A rapid, economical, reproducible, and simple direct spectrophotometric method was developed and validated for the assay of nitazoxanide in pharmaceutical formulations. Nitazoxanide concentration was estimated in water at 345 nm and pH 4.5. The method was suitable and validated for specificity, linearity, precision, and accuracy. There was no interference of the excipients in the determination of the active pharmaceutical ingredient. The proposed method was successfully applied in the determination of nitazoxanide in coated tablets and in powders for oral suspension. This method was compared to a previously developed and validated method for liquid chromatography to the same drug. There was no significative difference between these methods for nitazoxanide quantitation.

A new fluorescent sensor for determination of thorium by thin film of 2-(acetyloxy)-N-(5-nitro-2-thiazolyl)-benzamide embedded in sol–gel matrix

“…The high molar absorptivity of uorophore is preferred because it will increase light absorption at a given wavelength, thereby increasing sensitivity of the uorophore when it reacts with the target analyte. According to Malesuik et al, 37 the molar absorptivity of L is 2.3 Â 10 4 L mol À1 cm À1 . In addition to the advantages of high hydrophobicity and high molar absorptivity, L is also capable of preventing Th(IV) from undergoing the ion hydrolysis effect.…”

A Turn-On–Type Fluorescence Resonance Energy Transfer Eco-friendly Method for Nitazoxanide Quantification in Pharmaceutical Dosage Form and Spiked Plasma: Evaluation of Greenness Profile Using Different Assessment Tools

“…Upon applying ESA tool, one could compare the developed method with the reported ones. Only reported method [6] has got the same ESA score of the proposed one but by deep investigation of its details using AGREE as will be discussed later, the developed method has proved to be more eco-friendly method. All ESA scores of the developed method and the reported ones have been described in detail in Table 8.…”

“…Statistical Analysis After development and full validation of the proposed analytical method, it was successfully used for NTZ analysis in its commercial tablets. The results of the proposed method were statistically compared to a reported method [6]. The estimated values of both parameters did not exceed the theoretical values using student's t-test and F-test at 95% confidence level as shown in Table 4.…”

“…The drug must be monitored for quality assurance in preparations and to achieve the best therapeutic doses while limiting the risk of toxicity [3]. There are few analytical literature reviews for estimation of NTZ such as spectrophotometry [4][5][6], HPLC [7][8][9], HPTLC [4,10,11], and stripping voltammetry [12][13][14][15]. Also, some bioanalytical methods for quantitation of NTZ were reported [16][17][18].…”

Nitazoxanida: aspectos gerais, sistemas de liberação e potencial de reposicionamento da molécula

“…De acordo com Malesuik, Paim, Schapoval e Steppe (2010), a nitazoxanida tem baixa solubilidade aquosa, sendo considerada praticamente insolúvel, o que torna uma característica significante de sua molécula. Assim, existe a necessidade da busca pelo desenvolvimento de novas estratégias farmacêuticas de liberação e veiculação que melhorem a sua biodisponibilidade, visando sua estabilidade e fornecendo uma terapêutica mais eficaz, assim como a oportunidade de potencializar sua aplicação em diversos tipos de morbidades.…”