Perfil químico de cultivares de feijão (phaseolus vulgaris) pela técnica de high resolution magic angle spinning (HR-MAS)

Lião, Luciano M.; Choze, Rafael; Cavalcante, Pedro Paulo Araujo; Santos, Suzana C.; Ferri, Pedro Henrique; Ferreira, Antônio G.

doi:10.1590/s0100-40422010000300028

Cited by 8 publications

(7 citation statements)

References 14 publications

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“…Also, the application of multivariate analysis on the 1 H HR-MAS datasets permitted discrimination of the samples according to their geographical origin. This finding supports that the chemical composition of crops can be influenced by environmental conditions, such as rain and soil type, and HR-MAS can be an important tool to evaluate these changes [66,67].…”

Section: Grainssupporting

confidence: 76%

High-resolution magic angle spinning nuclear magnetic resonance in foodstuff analysis

Santos

Fonseca

Lião

et al. 2015

TrAC Trends in Analytical Chemistry

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Section: Grainssupporting

confidence: 76%

High-resolution magic angle spinning nuclear magnetic resonance in foodstuff analysis

Santos

Fonseca

Lião

et al. 2015

TrAC Trends in Analytical Chemistry

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“…[10][11][12][13] NMR provides, in particular, the appropriate technology and sufficient resources to enable reproducible analysis for molecular identification and quantification. Moreover, NMR has been a technique of great relevance in studies concerning plant defense mechanisms through structural proteomics by allowing for structure determination and the elucidation of structure-function relationships.…”

Section: Introductionmentioning

confidence: 99%

1H HRMAS NMR spectroscopy and chemometrics for evaluation of metabolic changes in citrus sinensis Caused by Xanthomonas axonopodis pv. citri

Silva¹,

Filho²,

Choze³

et al. 2012

J. Braz. Chem. Soc.

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A bactéria Xanthomonas axonopodis (Xac) causa uma das mais temidas e incuráveis doenças da citricultura: o cancro cítrico. Para compreender o mecanismo de resposta das laranjeiras quando atacadas pela Xac, folhas e frutos de Citrus sinensis foram diretamente avaliados por espectroscopia de RMN HRMAS (ressonância magnética nuclear de alta resolução com giro no ângulo mágico). Esta técnica permite a análise de amostras sem as laboriosas etapas de pré-tratamento, acessando as informações importantes sobre a sua composição química. As folhas de laranjeira e cascas do fruto investigadas neste estudo demonstraram as alterações bioquímicas causadas pela Xac. Auxiliado pelas análises quimiométricas, os resultados de RMN HRMAS apresentaram alterações relevantes no conteúdo de aminoácidos, carboidratos, ácidos orgânicos e terpenóides.Xanthomonas axonopodis (Xac) bacterium causes one of the most feared and untreatable diseases in citriculture: citrus canker. To understand the response mechanisms of orange trees when attacked by Xac, leaves and fruits of Citrus sinensis were directly evaluated by HRMAS NMR (high resolution magic angle spinning nuclear magnetic resonance) spectroscopy. This technique allows the analysis of samples without laborious pre-treatments and also allows access to important information about chemical composition of samples. The orange tree leaves and fruit peels investigated in this study demonstrated the biochemical changes caused by Xac. Aided by chemometric analysis, the HRMAS NMR results show relevant changes in amino acids, carbohydrates, organic acids and terpenoids content.

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“…12 Nuclear magnetic resonance (NMR) has been very valuable for the analysis of complex mixtures in several areas, such as food, metabolites and industrial product analyses, 13,14 in addition to provide a taxonomic classification of vegetal species, 15 considering that different species can produce different metabolites. 16 A recent option in NMR is the HRMAS (high resolution magic angle spinning) technique, which combines the advantages of NMR in solid state and in solution, 17,18 and has become useful for the direct analysis of many matrices such as seeds and leaves. Together to NMR, chemometric tools have been used as additional method for data exploration, such as: exploratory analysis (which enables the determination of the natural clusters), the consequential recognition of samples (which do not follow a certain pattern), the determination of the data information content and the verification of variables that better define the groups.…”

Section: Introductionmentioning

confidence: 99%

Discrimination of sugarcane according to cultivar by ¹H NMR and chemometric analyses

Filho

Silva

Choze

et al. 2012

J. Braz. Chem. Soc.

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Várias tecnologias para o desenvolvimento de novas cultivares de cana-de-açúcar têm focado, principalmente, no aumento da produtividade e maior resistência à doença. Cultivares de cana-deaçúcar são, geralmente, identificadas pela organografia das folhas e caule, análise de peroxidase e atividade da isoenzima esterase, proteínas solúveis totais e teor de sólidos solúveis. A ressonância magnética nuclear (RMN) associada às análises quimiométricas provou ser uma técnica valiosa para avaliação de plantas. Assim, este trabalho descreve o potencial das análises quimiométricas aplicadas a RMN de 1 H de alta resolução com giro no ângulo mágico (HRMAS) e em solução para investigação de cultivares de cana-de-açúcar. Para esta proposta, folhas de oito diferentes cultivares de cana-de-açúcar foram analisadas por espectroscopia de RMN de 1 H aliada à quimiometria. As técnicas empregadas apresentaram-se como ferramentas úteis para a distinção e classificação das diferentes cultivares, bem como para acessar as diferenças na composição química das cultivares.Several technologies for the development of new sugarcane cultivars have mainly focused on the increase in productivity and greater disease resistance. Sugarcane cultivars are usually identified by the organography of the leaves and stems, the analysis of peroxidase and esterase isoenzyme activities and the total soluble protein as well as soluble solid content. Nuclear magnetic resonance (NMR) associated with chemometric analysis has proven to be a valuable tool for cultivar assessment. Thus, this article describes the potential of chemometric analysis applied to 1 H high resolution magic angle spinning (HRMAS) and NMR in solution for the investigation of sugarcane cultivars. For this purpose, leaves from eight different cultivars of sugarcane were investigated by 1 H NMR spectroscopy in combination with chemometric analysis. The approach shows to be a useful tool for the distinction and classification of different sugarcane cultivars as well as to access the differences on its chemical composition.

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Perfil químico de cultivares de feijão (phaseolus vulgaris) pela técnica de high resolution magic angle spinning (HR-MAS)

Cited by 8 publications

References 14 publications

High-resolution magic angle spinning nuclear magnetic resonance in foodstuff analysis

High-resolution magic angle spinning nuclear magnetic resonance in foodstuff analysis

1H HRMAS NMR spectroscopy and chemometrics for evaluation of metabolic changes in citrus sinensis Caused by Xanthomonas axonopodis pv. citri

Discrimination of sugarcane according to cultivar by ¹H NMR and chemometric analyses

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