Produção de poli(3-hidroxibutirato) por Cupriavidus Necator em meio hidrolisado de amido de arroz com suplementação de óleo de soja em diferentes temperaturas

Abstract

Recebido em 19/2/09; aceito em 7/10/09; publicado na web em 24/2/10 PRODUCTION OF POLY(3-HYDROXYBUTYRATE) BY Cupriavidus necator IN HYDROLYZED RICE STARCH MEDIUM WITH SOYBEAN OIL SUPPLEMENTATION AT DIFFERENT TEMPERATURES. Poly (3-hydroxybutyrate) (P(3HB)) is a biopolymer, completely biodegradable, which has similar properties to fuel-based polymers. However to make it economically competitive it is necessary the study of cheap sources of substrate. The influence of hydrolyzed rice starch supplemented with soybean oil at different temperatures (30, 35 and 40 °C) was studied in the production of P(3HB) by C. necator. The percentage of P(3HB) produced in the cultures at 30, 35 °C was 30, 39% and 35, 43% without and with supplementation of oil, respectively. The culture at 40 °C showed no production phase due to a possible oxygen limitation. These results demonstrate that hydrolyzed rice starch supplemented with soybean oil increases the yield of P(3HB) and temperature of 35 ºC is the most favorable for biopolymer production.

POLY(3-HYDROXYBUTYRATE) PRODUCTION BY Cupriavidus necator SUPPLEMENTED WITH MINIEMULSIFIED SOYBEAN OIL

“…The increase in polymer accumulation, in terms of percentage of P(3HB) in the cells was 3.2% for the one supplemented with pure soybean oil and 9.7% for the one supplemented with the miniemulsified soybean oil. These values are in agreement with the results found by Dalcanton et al (2010), who obtained an increase of 22% in the P(3HB) accumulation when hydrolyzed rice starch was used as the main substrate supplemented with pure soybean oil, at an equivalent oleic acid concentration of 0.3 g.L -1 in C. necator cultures.…”

“…Since these fatty acids are present in many vegetable oils, some authors studied the supplementation of the culture medium with different oils and noticed that, just like with pure fatty acids, there was an increase in productivity and intracellular polymer accumulation (Lee et al, 2008;Ng et al, 2010;Dalcanton et al, 2010;Pradella et al, 2012). The biggest problem in the use these oils in culture media is heterogeneity since the oil, being hydrophobic, tend to remain on the surface even with intense agitation of the medium (Budde et al, 2011).…”

High cell density strategy for poly(3-hydroxybutyrate) production by Cupriavidus necator

“…High costs associated with production methods have, however, previously limited a more widespread use of PHA (Bengtssona et al, 2010). In recent years, research has focused on the development of alternative cost-effective processes for PHA production, including the use of low value substrates (agricultural or industrial waste or surplus feedstocks) (Aragão et al, 2009;Dalcanton et al, 2010;Lee et al, 2008;Reddy et al, 2003), mixed microbial cultures (reviewed by Dias et al, 2006;Serafim et al, 2008), and the development of efficient cultivation strategies, among which are cultures for high cell density production in the shortest time (Kulpreecha et al, 2009;Ahn et al, 2001;Khanna and Srivastava, 2005).…”