Quantitative determination of acetaminophen, phenylephrine and carbinoxamine in tablets by high-performance liquid chromatography

Abstract

An alternative methodology for analysis of acetaminophen (Ace), phenylephrine (Phe) and carbinoxamine (Car) in tablets by ion-pair reversed phase high performance liquid chromatography was validated. The pharmaceutical preparations were analyzed by using a C18 column (5 μm, 300 mm, 3.9 mm) and mobile phase consisting of 60% methanol and 40% potassium monobasic phosphate aqueous solution (62.46 mmol L-1) added with 1 mL phosphoric acid, 0.50 mL triethylamine and 0.25 g sodium lauryl sulfate. Isocratic analysis was performed under direct UV detection at 220 nm for Phe and Car and at 300 nm for Ace within 5 min

Development and validation of a rapid HPLC method for the determination of ascorbic acid, phenylephrine, paracetamol and caffeine using a monolithic column

“…Most of the above mentioned methods were used for the determination of paracetamol combinations like paracetamolascorbic acid, 8,14 paracetamol-phenylephrine hydrochloride 5,10,11,13,20,21 and paracetamol-caffeine. 2,4,9,12,16,17,22 The aim of this study was to develop and validate a rapid HPLC method for the determination of ascorbic acid, phenylephrine, paracetamol and caffeine using a monolithic column.…”

Development and validation of a generic high‐performance liquid chromatography for the simultaneous separation and determination of six cough ingredients: Robustness study on core–shell particles

“…Herein we reviewed only the reported HPLC methods for the analysis of the cited drugs in other mixtures. Ion pair chromatography was adopted using C 8 [8], C 18 [9], and cyano [10] columns. Micellar chromatography using either cetyltrimethylammonium bromide as cationic surfactant on C 8 column [11] or sodium dodecyl sulfate (SDS) as anionic surfactant on C 18 column [12] were reported.…”

Efectos tóxicos del paracetamol en la salud humana y el ambiente

“…La vía más reportada de contaminación es a través de su excreción inalterada en la orina y las heces; también por mecanismos antropogénica como la descarga accidental de APAP de uso humano; metabolismo post-consumo, eliminación de los hogares a través de los baños -principal fuente de contaminación de los ecosistemas-, e Impactos por actividades de la Planta de Tratamiento de Aguas Residuales (EDAR) (Bastos & Oliveira, 2009). Por eso no sorprende que en Estados Unidos, la principal fuente de fabricación y consumo de APAP, estudios hayan reportado 10 veces mayores intoxicaciones causantes de hepatoxicidad en humanos que en Europa y Asia; por su alto consumo, sólo en 2010 se formularon más de 30 millones de prescripciones médicas de APAP.…”