Determinação de ácido valpróico em soro por cromatografia líquida de alta eficiência com detector de arranjo de diodos (CLAE-DAD), após derivatização com brometo de fenacila

Antunes, Marina Venzon; Nagel, V.; Linden, Rafael; Werlang, Helenice Orosco; Hermes, Daniela

doi:10.1590/s0100-40422009000500027

Cited by 6 publications

(1 citation statement)

References 8 publications

(13 reference statements)

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“…The chemical structure of CLO ((2-[2, 6-dichlorophenyl] amino)-2-imidazoline) is shown in Figure 1. Moreover, CLO is considered as one of narrow therapeutic index (NTI) drugs, which minor errors in their dosing can provide the death of patients [5][6][7]. In this sense, an efficient determination of CLO in pharmaceutical samples is extremely important for quality control of this drug.…”

Section: Introductionmentioning

confidence: 99%

Simple and rapid determination of clonidine in pharmaceutical samples by voltammetry using a bare glassy carbon electrode

et al. 2023

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This work presents, for the first time, the voltammetric behavior of clonidine (CLO) drug and its determination, using an unmodified glassy carbon electrode (GCE). CLO exhibited only an irreversible oxidation process on the GCE, with peak potential at +0.85 V in pH 12 (vs Ag/AgCl). CLO oxidation process is pH‐dependent and the electrochemical mechanisms on the GCE were proposed in acidic and basic medium. The determination of CLO was optimized in 0.1 mol L−1 phosphate buffer solution at pH 12.0 using differential pulse voltammetry (DPV), which provides a good linear range (0.65 to 106.00 μmol L−1) and low theoretical limit of detection (0.14 μmol L−1) for the quality control of this drug in pharmaceutical samples. In addition, stable responses of CLO at the GCE were obtained in the same day (RSD = 3.4 %; n = 5) and different days (RSD = 2.0 %; n = 3). Moreover, the determination of CLO in a pharmaceutical formulation using the proposed GCE‐DPV method presented good accuracy, since the recovery was close to 100 % and the dosing result was in agreement with an official method (HPLC‐UV). The proposed method demonstrates a good analytical performance for CLO determination in pharmaceutical samples, providing a faster, simpler and lower‐cost alternative for quality control of CLO than other reported methods.

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Section: Introductionmentioning

confidence: 99%

Simple and rapid determination of clonidine in pharmaceutical samples by voltammetry using a bare glassy carbon electrode

et al. 2023

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A new derivatization method for the determination of valproic acid in serum using tetramethylammonium hydroxide as a catalyst

Gui-fu

Zhao

Sun

et al. 2018

Biomedical Chromatography

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Valproic acid (VPA) pharmacokinetics is highly variable and monitoring of blood levels is necessary to determine its appropriate dosage. This study aimed to establish and validate a novel derivatization method for the determination of VPA. The method was based on the catalytic effect of tetramethylammonium hydroxide using 2,4′‐dibromoacetophenone as a derivatization reagent. After derivatization, samples were injected into the HPLC system for analysis. The method showed a good linearity in the range of 1.0–200.7 μg mL−1, and the limit of quantification was 1 μg mL−1. All values of the accuracy and relative standard deviations were acceptable for the analyses of biological samples. The recoveries were in the range from 91.6 to 97.4% for VPA with RSD <3.9%. A novel and high conversion‐rate derivatization method has been developed and validated for the determination of VPA in human serum. It can be applied to the analysis of VPA in clinic serum samples.

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Simultaneous determination of valproic acid and its main metabolite in human plasma using a small scale dispersive liquid–liquid microextraction followed by gas chromatography–flame ionization detection

et al. 2018

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Determinação de ácido valpróico em soro por cromatografia líquida de alta eficiência com detector de arranjo de diodos (CLAE-DAD), após derivatização com brometo de fenacila

Cited by 6 publications

References 8 publications

Simple and rapid determination of clonidine in pharmaceutical samples by voltammetry using a bare glassy carbon electrode

Simple and rapid determination of clonidine in pharmaceutical samples by voltammetry using a bare glassy carbon electrode

A new derivatization method for the determination of valproic acid in serum using tetramethylammonium hydroxide as a catalyst

Simultaneous determination of valproic acid and its main metabolite in human plasma using a small scale dispersive liquid–liquid microextraction followed by gas chromatography–flame ionization detection

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